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光谱法研究灿烂甲酚蓝与DNA的相互作用 总被引:2,自引:0,他引:2
利用紫外-可见(UV/Vis)光谱法研究了小分子染料灿烂甲酚蓝(BCB)与小牛胸腺DNA(ct DNA)的相互作用。实验表明,DNA在低浓度时,BCB能结合DNA形成1∶1的化合物,结合常数为4.3×104 L·mol-1,DNA与BCB的主要结合方式是“静电作用”方式;而在较高浓度时,则变成“嵌入作用”方式。为了进一步证明BCB分子嵌入到DNA, 利用了羟丙基-β环糊精(HP-β-CD)与BCB的包合行为来研究。实验证明,BCB分子能进入HP-β-CD的疏水性空腔形成包合物, 稳定常数为1.98×103 L·mol-1;高浓度DNA存在时,BCB分子从HP-β-CD空腔中离解而与DNA发生嵌入作用。据此可推断出BCB分子能嵌入到DNA的双螺旋沟槽中。BCB分子属经典的嵌入剂。 相似文献
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Spectrofluorimetric Determination of Reduced Glutathione Using Organic Nanoparticle Probes 总被引:1,自引:0,他引:1
Introduction Reduced glutathione (GSH) is a very important tripeptide.1 GSH widely exists in living tissues. In ani-mal organization, the concentration of free glutathione is in the range 0.5—10.0 mmol/L. Usually over 99% of glutathione is present in the reduced form in all organ-isms.2 Intermediates of GSH biosynthesis such as cys-teine, g-glutamyl-cysteine (g-Glu-Cys) or cysteinyl-gly- cine (Cys-Gly) also occur in the cell but at much lower concentrations.3 GSH plays an important bio… 相似文献
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