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Suihan Feng Dr. Vibor Laketa Frank Stein Dr. Anna Rutkowska Dr. Aidan MacNamara Dr. Sofia Depner Prof. Ursula Klingmüller Dr. Julio Saez‐Rodriguez Priv.‐Doz. Dr. Carsten Schultz 《Angewandte Chemie (International ed. in English)》2014,53(26):6720-6723
Chemical dimerizers are powerful tools for non‐invasive manipulation of enzyme activities in intact cells. Here we introduce the first rapidly reversible small‐molecule‐based dimerization system and demonstrate a sufficiently fast switch‐off to determine kinetics of lipid metabolizing enzymes in living cells. We applied this new method to induce and stop phosphatidylinositol 3‐kinase (PI3K) activity, allowing us to quantitatively measure the turnover of phosphatidylinositol 3,4,5‐trisphosphate (PIP3) and its downstream effectors by confocal fluorescence microscopy as well as standard biochemical methods. 相似文献
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Laketa Petra N. Nastić Aleksandar S. Ristić Miroslav M. 《Mediterranean Journal of Mathematics》2018,15(1):1-14
Mediterranean Journal of Mathematics - Let $$C_{\varphi }$$ be the composition operator with monomial symbol $$\varphi (z)=z^m$$ , $$z\in \mathbb {D}$$ , for some positive integer m. In this... 相似文献
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