黄鳝DEAD-box家族PL10基因的克隆与序列分析

通过PCR克隆的方法，从黄鳝(Monopterus albus)中得到两个PL10基因的cDNA片段Mo PL10A和Mo PL10B，长度均为1．127kb，推测其编码375个氨基酸的蛋白片段．结合其他PL10类同源物序列，对这两条cDNA进行了分析和初步的功能推测．根据此片段的氨基酸序列构建的系统发育树与形态分类结果一致．在不同组织中的RT—PCR结果表明Mo PL10A和Mo PL10B的mRNA在各组织中的分布有差异．     

MEIOTIC CHROMOSOME BEHAVIOR IN FEMALE INTERSEXES OF ARTIFICIAL TRIPLOID TRANSPARENT-COLORED CRUCIAN CARP

Chromosome behavior in meiosis was studied by ail-drying, C-banding and surface-spreading methods in female intersexes of artificial triploid transparent-colored crucian carp (Corassius auratus). Chromosome pairing and contraction were obviously asynchronous. The preferential pairing of two homologous chromosomes was the major pattern of chromosome pairing, and a few triple pairing, repeated pairing, telomere or centromere associating and multiple pairing were also observed in the pachytene cells. The metaphase I cells were main-ly composed of univalents, bivalents and trivalents, as well as a few of other multivalents, such as tetravalents, pentavalents, hexavalents and heptavalents, were also found in some metaphase I cells. The chromosome elements including uni-, bi-, tri- and other multivalents varied considerably among the metaphase I cells, and the associating patterns of multivalents were also diverse. Some 6 n and 12 n cells, in which premeiotic endomitosis occurred once or twice, were found at     

深情怀念恩师余先觉教授

1994年8月10日晨,中国遗传学界一颗巨星陨落,我们的恩师,著名的细胞遗传学家余先觉教授逝世。噩耗传来,我们悲痛万分,为遗传学界痛失一位杰出的前辈而惋惜,也为我们痛失一位德高望重的导师而悲怆。缅怀往事,余老的伟岸人格、高尚情操和治学之道将永远铭刻在我们心中。 余先觉先生1909年11月出生于湖南省长沙县,1935年毕业于武汉大学生物学系并留校任教。他先后担任武汉大学校务委员会委员、校学术委员会委员,并曾任中国遗传学会理事,中国细胞生物学会理事、中国遗传学动物遗传委员会委员,《遗传学报》、《遗传》和《水生生物学报》等期刊的编委,并数届连任湖北省遗传学会理事长。 余先觉先生于1946年公费留学,赴美国加州理工学院,在现代遗传学泰斗、诺贝尔奖金获得者摩尔根教授的实验室攻读博士     

人工三倍体水晶彩鲫雌性型间性体减数分裂的染色体行为

本文报道了人工三倍体水晶彩鲫雌性型间性体减数分裂过程以及染色体配对和联合等行为.染色体配对和凝缩表现有明显的非同步性,在配对形式上,主要表现为两条同源染色体的优先配对,也见有少数“三联配对”、重复配对、端粒或着丝粒联合和复合配对等形式.中期Ⅰ细胞主要由单价体、二价体和三价体以及少量多价体组成,且细胞间变化很大,多价体的联合形式也较为多样.经历了一次或两次核内有丝分裂形成的六倍体和十二倍体细胞也能进入减数分裂,其配对和联合形式基本上与三倍体相似.