The Bioactive Peptide SL-13R Expands Human Umbilical Cord Blood Hematopoietic Stem and Progenitor Cells In Vitro

Hematopoietic stem and progenitor cell (HSPC) transplantation is a curative treatment of hematological disorders that has been utilized for several decades. Although umbilical cord blood (UCB) is a promising source of HSPCs, the low dose of HSPCs in these preparations limits their use, prompting need for ex vivo HSPC expansion. To establish a more efficient method to expand UCB HSPCs, we developed the bioactive peptide named SL-13R and cultured UCB HSPCs (CD34+ cells) with SL-13R in animal component-free medium containing a cytokine cocktail. Following 9 days of culture with SL-13R, the numbers of total cells, CD34+, CD38− cells, and hematopoietic stem cell (HSC)-enriched cells were significantly increased relative to control. Transplantation of cells cultured with SL-13R into immunodeficient NOD/Shi-scid/IL-2Rγ knockout mice confirmed that they possess long-term reconstitution and self-renewal ability. AHNAK, ANXA2, and PLEC all interact with SL-13R. Knockdown of these genes in UCB CD34+ cells resulted in reduced numbers of hematopoietic colonies relative to SL-13R-treated and non-knockdown controls. In summary, we have identified a novel bioactive peptide SL-13R promoting expansion of UCB CD34+ cells with long-term reconstitution and self-renewal ability, suggesting its clinical use in the future.     

Thermal conductivity modeling of MgO/EG nanofluids using experimental data and artificial neural network

The application of nanofluids in energy systems is developing day by day. Before using a nanofluid in an energy system, it is necessary to measure the properties of nanofluids. In this paper, first the results of experiments on the thermal conductivity of MgO/ethylene glycol (EG) nanofluids in a temperature range of 25–55 °C and volume concentrations up to 5 % are presented. Different sizes of MgO nanoparticles are selected to disperse in EG, including 20, 40, 50, and 60 nm. Based on the results, an empirical correlation is presented as a function of temperature, volume fraction, and nanoparticle size. Next, the model of thermal conductivity enhancement in terms of volume fraction, particle size, and temperature was developed via neural network based on the measured data. It is observed that neural network can be used as a powerful tool to predict the thermal conductivity of nanofluids.     

The effects of channel diameter on flow pattern,void fraction and pressure drop of two-phase air–water flow in circular micro-channels

Two-phase air–water flow characteristics are experimentally investigated in horizontal circular micro-channels. Test sections are made of fused silica. The experiments are conducted based on three different inner diameters of 0.53, 0.22 and 0.15 mm with the corresponding lengths of 320, 120 and 104 mm, respectively. The test runs are done at superficial velocities of gas and liquid ranging between 0.37–42.36 and 0.005–3.04 m/s, respectively. The flow visualisation is facilitated by systems mainly including stereozoom microscope and high-speed camera. The flow regime maps developed from the observed flow patterns are presented. The void fractions are determined based on image analysis. New correlation for two-phase frictional multiplier is also proposed for practical applications.     

An alternative approach for the estimation of equivalent temperature in gas chromatography

The temperature at which the isothermal retention time (tRiso) is equal to the temperature-programmed retention time (tRTPGC) or the isothermal retention index (Iiso) is equal to the temperature-programmed retention index (ITPGC) is defined as the equivalent temperature (Teq). The Teq of one-, two-, three-, and four-step temperature-programmed gas chromatography (TPGC) of unsaturated fatty acid methyl esters (FAMEs) from Chinese mustard seed oil is calculated. All of the tR values obtained at the Teq (tRTeq) are very close to the tRTPGC. The highest difference for each chromatogram is less than 0.5%. The slight deviation may partly arrive from the difficulty in setting the desired carrier gas flow rate. Also, good agreement among equivalent chain length values determined by different methods is observed, including the graphical method at the Teq. Therefore, it is speculated that the proposed method may facilitate the GC identification of FAMEs as well as other organic compounds in TPGC by using the available isothermal retention index database.     

Experimental Study of the Bottom Structure Effect on the Damping of Standing Surface Waves in a Rectangular Vessel

The damping coefficient is estimated for standing surface waves in a rectangular vessel: (1) with a smooth horizontal rigid bottom, (2) with developed sandy bottom structures, (3) with a profiled rigid bottom, and with thin bottom layers of (4) fine-grained sand and (5) glass spheres. The results obtained are compared with available theoretical models.     

Measurement of temperature-dependent thermal conductivity and viscosity of TiO2-water nanofluids

Nanofluid is an innovative heat transfer fluid with superior potential for enhancing the heat transfer performance of conventional fluids. Many attempts have been made to investigate its thermal conductivity and viscosity, which are important thermophysical properties. No definitive agreements have emerged, however, about these properties. This article reports the thermal conductivity and dynamic viscosity of nanofluids experimentally. TiO₂ nanoparticles dispersed in water with volume concentration of 0.2–2 vol.% are used in the present study. A transient hot-wire apparatus is used for measuring the thermal conductivity of nanofluids whereas the Bohlin rotational rheometer (Malvern Instrument) is used to measure the viscosity of nanofluids. The data are collected for temperatures ranging from 15 °C to 35 °C. The results show that the measured viscosity and thermal conductivity of nanofluids increased as the particle concentrations increased and are higher than the values of the base liquids. Furthermore, thermal conductivity of nanofluids increased with increasing nanofluid temperatures and, conversely, the viscosity of nanofluids decreased with increasing temperature of nanofluids. Moreover, the measured thermal conductivity and viscosity of nanofluids are quite different from the predicted values from the existing correlations and the data reported by other researchers. Finally, new thermophysical correlations are proposed for predicting the thermal conductivity and viscosity of nanofluids.     

A tube-by-tube reduction method for simultaneous heat and mass transfer characteristics for plain fin-and-tube heat exchangers in dehumidifying conditions

This study proposed a new method, namely a tube-by-tube reduction method to analyze the performance of fin-and-tube heat exchangers having plain fin configuration under dehumidifying conditions. The mass transfer coefficients which seldom reported in the open literature, are also presented. For fully wet conditions, it is found that the reduced results for both sensible heat transfer performance and the mass transfer performance by the present method are insensitive to change of inlet humidity. Unlike those tested in fully dry condition, the sensible heat transfer performance under dehumidification is comparatively independent of fin pitch. The ratio of the heat transfer characteristic to mass transfer characteristic (h_c,o/h_d,o C_p,a) is in the range of 0.6~1.0, and the ratio is insensitive to change of fin spacing at low Reynolds number. However, a slight drop of the ratio of (h_c,o/h_d,o C_p,a) is seen with the decrease of fin spacing when the Reynolds number is sufficient high. This is associated with the more pronounced influence due to condensate removal by the vapor shear. Correlations are proposed to describe the heat and mass performance for the present plate fin configurations. These correlations can describe 89% of the Chilton Colburn j-factor of the heat transfer (j_h) within 15% and can correlate 81% of the Chilton Colburn j-factor of the mass transfer (j_m) within 20%.     

An investigation of a model of the flow pattern transition mechanism in relation to the identification of annular flow of R134a in a vertical tube using various void fraction models and flow regime maps

In the present study, new experimental data are presented for literature on the prediction of film thickness and identification of flow regime during the co-current downward condensation in a vertical smooth copper tube having an inner diameter of 8.1 mm and a length of 500 mm. R134a and water are used as working fluids in the tube side and annular side of a double tube heat exchanger, respectively. Condensation experiments are done at mass fluxes of 300 and 515 kg m^?2 s^?1. The condensing temperatures are between 40 and 50 °C; heat fluxes are between 12.65 and 66.61 kW m^?2. The average experimental heat transfer coefficient of the refrigerant HFC-134a is calculated by applying an energy balance based on the energy transferred from the test section. A mathematical model by Barnea et al. based on the momentum balance of liquid and vapor phases is used to determine the condensation film thickness of R134a. The comparative film thickness values are determined indirectly using relevant measured data together with various void fraction models and correlations reported in the open literature. The effects of heat flux, mass flux, and condensation temperature on the film thickness and condensation heat transfer coefficient are also discussed for the laminar and turbulent flow conditions. There is a good agreement between the film thickness results obtained from the theoretical model and those obtained from six of 35 void fraction models in the high mass flux region of R134a. In spite of their different valid conditions, six well-known flow regime maps from the literature are found to be predictive for the annular flow conditions in the test tube in spite of their different operating conditions.     

Microstructure of the solid phase in fluidized beds for non-Stokes regimes

Experiments on two-dimensional fluidized beds of glass beads are presented for two fluids of typical Reynolds numbers of about 1 and a few hundred. The presence of inertial effects leads to the formation of wakes behind the particles and consequently to an anisotropy of the microstructure. The microstructures, studied by video imaging, are characterized by pair probability distribution functions and structure factors.     

Effect of fin pattern on the air-side performance of herringbone wavy fin-and-tube heat exchangers

In the present study, new experimental data on the air-side performance of fin-and-tube heat exchangers having herringbone wavy fin configuration are presented. Different from most previous studies, the present experiments have been performed to determine the effects of fin patterns and edge corrugations on the air-side performance of the heat exchangers. The experimental apparatus consists essentially of a well-insulated open wind tunnel and herringbone wavy fin-and-tube heat exchangers made from aluminium wavy finned, copper tube. Two types of wavy fin patterns commonly in industrial use are investigated. Air and hot water are used as working fluids in air-side and tube-side, respectively. From the experimental results, it is found that the fin pattern has a significant effect on the heat transfer and flow characteristics. The corrugation at the fin edge enables the Colburn factor to decrease but it has almost no effect on the friction factor.