Detection of Amines in Lamb Spoilage by Optical Waveguide Sensor Based on Bromophenol Blue-Silicon Composite Film

We herein report the development of a bromophenol blue(BPB)-silicone composite film/K^+-exchange glass optical waveguide(OWG)sensor for the detection of amines produced during the spoilage of lamb.the optical and structural properties of the sensitive thin film were studied by ultra violet-visible(UV-Vis)spectroscopy,and the light source of the OWG detecting system was selected.Gas sensing measurements showed that the sensor exhibited a good selectivity,higli sensitivity,and short response-recovery time towards volatile amine gases in the 0.00117一11.72 mg/g range.The as-prepared optical waveguide device was subsequently applied in the determination of gases(namely trimethylamine,dimethylamine,and ammonia)emitted from the lamb samples(5g)stored at room temperature(25℃)and in a refrigerator(5℃)for 0—4 d,and the total volatile basic nitrogen(TVB-N)contents were detected by UV-Vis spectroscopy,and the results were compared witli those obtained using our detector.It was found that the sensing element was capable of detecting mixed gases produced by the decomposition of lamb samples in a refrigerator for 0.5 h,where the TVB-N content was lower than 35μg/g.     

A self-powered and drug-free diabetic wound healing patch breaking hyperglycemia and low H2O2 limitations and precisely sterilizing driven by electricity

Accelerating diabetes-related chronic wound healing is a long-sought-after goal in diabetes management. However, therapeutic strategies based on antibiotics or catalysts still face great challenges to break the limitations of antimicrobial resistance, low H₂O₂ and the blocking effect of bacterial biofilms on antibiotic/catalyst penetration. Herein, we reported a glucose biofuel cell-powered and drug-free antibacterial patch, which consisted of an MAF-7 protected glucose oxidase/horseradish peroxidase anode and a horseradish peroxidase cathode, for treating diabetic wounds. This self-powered patch could take high blood glucose as fuel to generate electricity and abundant reactive oxygen species (ROS) in situ, synergistically regulating local hyperglycemia and breaking the limitations of insufficient ROS caused by low H₂O₂ levels. In particular, the electric field created by the GBFC could drive the negatively charged bacteria to adhere firmly to the electrode surface. As a result, the ROS produced in situ on the electrodes was localized to the bacteria, realizing precise sterilization. In vivo experiments confirmed that this self-powered patch enabled the wounds on diabetic mice to take a mere 10 days to eliminate inflammation and form mature skin with new hair follicles, demonstrating its great potential in treating bacteria-infected diabetic wounds.

A GBFC-powered antibacterial patch which can break low H₂O₂ limitations and precisely sterilize driven by electricity was created to treat bacteria-infected diabetic wounds without depending on any exogenous drugs.