Fusion Proteins Cpn10-E^rns with Properties of Generating CSFV-Neutralized Antibodies

IntroductionClassical swine fever(CSF), included in the listA of the Office International des Epizooties(OIE), is ahighly contagious disease of domestic pigs and is con-sidered as the mostharmful disease occurring in pigs allover the world[1]. Outbreaks o…     

光学元件狭缝柔性调节机构的设计与分析

设计了一种狭缝柔性结构的光学元件调节机构,使光学元件在具备较高调节精度的同时,保持较高的导向精度。采用弹性力学应力函数法分析了狭缝柔性结构的刚度,以径向刚度与轴向刚度的比值为目标函数,对狭缝柔性结构尺寸参数进行了优化,在不超过柔性结构材料屈服应力等约束条件下,刚度比最优值达到1 573.6,较大的刚度比值可以减小调节机构的耦合位移,从而提高机构的导向精度。该结构加工装配方便,可实现三自由度(θx-θy-Z)调节。对优化后的柔性结构进行仿真分析,结果表明:径向刚度与轴向刚度比值的仿真值为1 660.4,解析值与仿真值误差为5.23%,证明了刚度分析方法的有效性。优化后的结构,轴向调节行程为2.09 mm,绕x轴偏转角度调节行程为±16.6 mrad,绕y轴偏转角度调节行程可达到±14.4 mrad,满足光学元件调节的大行程要求。     

2-羟基-1-萘醛缩乙二胺Schiff碱及其过渡金属配合物的合成和表征

Schiff 碱配体包含着酚氧桥氧原子和亚胺氮原子这类强电子给体,他们与过渡金属原子有较强的配位能力,在催化、抗癌、抗菌、抗病毒等方面都有广泛的应用[1-4],受到人们的高度关注.     

光学元件双转子抛光技术中类高斯型去除函数建模与逼近

在Preston假设基础上,研究并推导了光学元件抛光过程中"花瓣"型截面磨头在双转子运动形式下的去除函数.提出了"花瓣"型截面磨头的离散点弧长表达方法,并讨论了该方法的适用范围;基于离散点弧长法,推导了"花瓣"型截面磨头在双转子运动形式下的去除函数表达式;根据可调步长式曲线逼近原理研究了类高斯型目标去除函数的逼近算法;并得到了类余弦型去除函数的双转子抛光模参量.从而证明了双转子抛光技术可以得到类高斯型去除函数.     

能见度仪探测光束平行度检测方法

为实现能见度计算中消光系数的正确测量,论述了光源平行度在能见度测量中的重要作用,设计了探测光束发散角检测方法与测量装置。该装置包括定焦成像系统和移动光源的直线系统,利用成像关系与空间几何关系推导了发散角表达式。以紫外LED作为实验光源,设计完成了测量系统成像装置与控制软件,计算得到水平方向与垂直方向发散角,利用所得发散角分析探测光束。实验结果表明,紫外LED光源的光束发散角水平与垂直方向分别为7.8和6.9,经比较得到未加入平行度的能见度与加入后的比值为0.51。该方法测得的能见度值更接近于实际值。     

小鼠早期胚胎肢体发育中的视黄酸诱导的视黄酸代谢

给怀孕10 d的小鼠胃灌流视黄酸(100 mgRA/kg体重)6和24 h后,对视黄酸合成酶基因Raldh2和代谢酶基因Cyp26a1,Cyp26b1在胚胎肢体的表达进行了分析.Cyp26a1和Cyp26b1在灌流6 h,表达增加;灌流24 h,表达恢复到正常水平.Raldh2在此时间段表达降低.这些结果提示:在小鼠早期胚胎肢体发育中,存在视黄酸诱导的视黄酸代谢.     

视黄酸合成酶基因Raldh2敲除鼠肢体发育起始的挽救

视黄酸合成酶基因Raldh2敲除鼠胚胎心脏膨大,没有肢体发育的起始.肌细胞增强子基因Mef2C敲除鼠胚胎心脏较小,但肢体的发育起始正常.Raldh2/Mef2C双基因敲除鼠与Raldh2敲除鼠形态相似.Tbx5在Raldh2/Mef2C基因敲除鼠第9天的胚胎表达表明,双基因敲除鼠胚胎没有肢体的发育.这一结果表明Mef2C敲除鼠没有挽救Raldh2敲除鼠肢体发育的起始.     

DHL细胞中5-ALA代谢PpIX的双光子荧光光谱

双光子激发生物组织荧光,激发光仅作用于焦点区域,对生物样品的光漂白性和光毒性都很小,因而双光子荧光显微技术已成为细胞生物学研究的一种新技术。文章采用波长为820 nm飞秒激光激发孵育有5-ALA的DHL细胞,在激光扫描显微镜的Lambda模式中获得单个DHL细胞的双光子荧光光谱,并测量DHL细胞内积聚的卟啉九(PpIX)特征荧光值。获得了浓度分别为2, 4和10 mmol·L-1的5-ALA溶液中,细胞代谢的PpIX含量随孵育时间的变化情况。DHL细胞内积聚的PpIX处于动态变化过程,并呈现出两阶段性的特点：细胞内积聚的PpIX含量随着孵育时间增长而增加,在3 h附近达到最大值,随后随着孵育时间增长反而下降。结果表明,基于激光扫描显微的双光子荧光光谱可成为DHL细胞等白血病细胞摄取5-ALA并生成PpIX的动力学研究的有效方法。     

Studies on Model Interaction of Keggin-type Polyoxometalates with Nucleic Acid

Keggin anions behave differently from each other when they react with nucleic acids. The molybdenum series exhibits oxidative cleavage activity towards AMP and DNA. The mechanism of AMP damage and DNA cleavage caused by the molybdenum series is mainly oxidation and the oxidation sites are on the ribose parts other than on the adenine parts, while the hydrolysis probably makes significant contributions to the cleavage of DNA and to the damage of AMP caused by the tungsten series.     

Structure and Properties of Self-reinforced Material Made from Ultra-high Molecular Weight Polyethylene-montmorillonite Nanocomposite

High-strength and high-modulus ultra-high molecular weight polyethylene (UHMWPE), named self-re-inforced material, was obtained by the elongation of UHMWPE-montmorillonite nanocomposite at melting temperature. According to the scanning electron microscope (SEM) analysis, a great deal of fibrillar texture formed in the direction of elongation, and the tensile fractured surface was similar to that of highly oriented fiber. The transmission electron microscope (TEM) and selective area electron diffraction (SAED) analyses reveal that the reinforced phase of the self-reinforced material is an extended chain crystal and its size is about 50--200 nm wide and several microns long, and the montmorillonite layers are broken up to pieces in the size from 100 to 10 nm. The broken layers which have a huge surface area interacting strongly with macro-molecules reduces the entanglement density of UHMWPE and induces the chain orientation in flow field. It is supposed that the astriction of montmorillonite layers to polyethylene chains is not only end-tethered but also side-tethered. The differential scan calorimetry(DSC) analysis shows that there are two endothermal peaks for the self-reinforced material, of which the peak at a higher temperature(136.4℃) is ascribed to the melt-ing of the reinforced phase.