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1.
Watanabe G Umetsu K Yuasa I Sato M Sakabe M Naito E Yamanouchi H Suzuki T 《Electrophoresis》2001,22(3):418-420
We present a simple and rapid polymerase chain reaction (PCR)-based technique, termed consumed allele-specific primer analysis (CASPA), as a new strategy for single nucleotide polymorphism (SNP) analysis. The method involves the use of labeled allele-specific primers, differing in length, with several noncomplementary nucleotides added in the 5'-terminal region. After PCR amplification, the amounts of the remaining primers not incorporated into the PCR products are determined. Thus, nucleotide substitutions are identified by measuring the consumption of primers. In this study, the CASPA method was successfully applied to ABO genotyping. In the present method, the allele-specific primer only anneals with the target polymorphic site on the DNA, so it is not necessary to analyze the PCR products. Therefore, this method is only little affected by modification of the PCR products. The CASPA method is expected to be a useful tool for typing of SNPs. 相似文献
2.
Umetsu K Tanaka M Yuasa I Adachi N Miyoshi A Kashimura S Park KS Wei YH Watanabe G Osawa M 《Electrophoresis》2005,26(1):91-98
We present a reliable, rapid, and economical multiplex amplified product-length polymorphism (APLP) method for analyzing the haplogroup-diagnostic mitochondrial single-nucleotide polymorphisms (mtSNPs) in East Asian populations. By examining only 36 haplogroup-specific mtSNPs in the coding region by using four 9-multiplex polymerase chain reaction (PCR) and subsequent electrophoresis, we could safely assign 1815 individuals from 8 populations of Japanese, Korean, Chinese, and Germans to 45 relevant haplogroups. This multiplex APLP analysis of coding-region mtSNPs for haplogrouping is especially useful not only for molecular phylogenetic studies but also for large-scale association studies due to its rapid and economical nature. This is the first panel of mtSNPs in the coding region to be used for haplogrouping of East Asian populations. 相似文献
3.
Human alpha 2HS-glycoprotein (AHSG) phenotypes were studied by isoelectric focusing of native and desialyzed samples followed by passive immunoblotting. At present a total of 15 different alleles could be distinguished. The usefulness of AHSG polymorphism in the field of forensic hemogenetics was demonstrated by markedly high variability at AHSG locus, reliability of phenotyping results and stability in blood stains. 相似文献
4.
Suyama Kyozo Fukazawa Yoshitaka Umetsu Yoshiyaki 《Applied biochemistry and biotechnology》1994,(1):871-879
The egg shell membrane (ESM) is an intricate lattice network of stable and water-insoluble fibers with high surface area.
ESM accumulates and eliminates various heavy metal ions from dilute aqueous solution with high affinity and in short contact
time, depending on pH and characteristics of the individual ion. Under certain conditions, the level of precious ions, Au,
Pt, and Pd accumulation approaches 55, 25, and 22% of dry wt of ESM, respectively. Also uranium uptake 30% of that of ESM.
Experiments suggested that ESM is promising to use for the purpose of removal/recovery of metals and water pollution control. 相似文献
5.
In the course of a population study of alpha 1-antitrypsin polymorphism by separator isoelectric focusing, a variant phenotype having a somewhat narrower spacing than PI M1M3 was observed in a Japanese blood donor. Family studies by hybrid isoelectric focusing in a carrier ampholyte-supplemented immobilized pH gradient from 4.35-4.65 revealed that the products of the responsible gene, PI*Mtoyoura, were extremely close but slightly cathodal to those of PI*M1. The difference in isoelectric point between them corresponded to the resolving limit of isoelectric focusing. For this reason, although the propositus' father was deduced to have the genotype PI*M1/PI*Mtoyoura, the products of these two genes failed to form a double band pattern. Thus, the gene frequency for PI*Mtoyoura was unknown. These findings, however, indicate that a further microheterogeneity in the PI M subtype exists at least in the Japanese. 相似文献
6.
Takanari Togashi Mitsuo Umetsu Takashi Naka Satoshi Ohara Yoshiharu Hatakeyama Tadafumi Adschiri 《Journal of nanoparticle research》2011,13(9):3991-3999
The assembly of metal oxide nanoparticles (NPs) on a biomolecular template by a one-pot hydrothermal synthesis method is achieved
for the first time. Magnetite (Fe3O4) nanoneedles (length: ~100 nm; width: ~10 nm) were assembled on cyclic-diphenylalanine (cFF) nanorods (length: 2–10 μm; width:
200 nm). The Fe3O4 nanoneedles and cFF nanorods were simultaneously synthesized from FeSO4 and l-phenylalanine by hydrothermal synthesis (220 °C and 22 MPa), respectively. The samples were analyzed by powder X-ray diffraction
(XRD), Fourier transform infrared spectroscopy (IR), transmission electron microscopy (TEM), and superconducting quantum interference
device (SQUID) magnetometry. Experimental results indicate that Fe3O4 nanoneedles were assembled on cFF nanorods during the hydrothermal reaction. The composite contained 3.3 wt% Fe3O4 nanoneedles without any loss of the original magnetic properties of Fe3O4. 相似文献
7.
8.
Kazuteru Umetsu 《Tetrahedron letters》2008,49(49):7046-7049
Treatment of ortho-alkynylbenzoic acid esters with excess amounts of EtOH in the presence of a gold catalyst results in the liberation of alcohols or phenols in high yields under mild conditions. The protection of alcohols and phenols proceeds smoothly by use of ortho-alkynylbenzoic acid or ortho-iodobenzoyl chloride. Highly chemoselective deprotections are described. 相似文献
9.
H. Yako T. Kanomata K. Endo R.Y. Umetsu A. FujitaR. Kainuma H. NishiharaK.R.A. Ziebeck 《Physica B: Condensed Matter》2012,407(3):311-315
X-ray powder diffraction and magnetization measurements have been carried out on Rh2Mn1+xSn1−x (0≤x≤0.3) alloys. The alloys, which crystallize in the L21 structure, were found to exhibit ferromagnetic behavior. The lattice constant a at room temperature decreases with increasing x, whereas the Curie temperature TC decreases linearly. At 5 K the magnetic moment per formula unit first increases with increasing x and then saturates for x≥0.2. The experimental results are discussed in terms of the influence of the Mn-Mn exchange interactions between the Mn atoms on the Sn and Mn sites. 相似文献
10.
The genetic polymorphisms of orosomucoid, ORM1 and ORM2, were analyzed by isoelectric focusing on polyacrylamide gels and subsequent immunoprinting. Sera from 600 unrelated individuals in Taiwan and Japan were examined. Five new alleles, designated ORM1*7, ORM1*8, ORM1*4.2, ORM2*8 and ORM2*10, were observed together with common and rare alleles that have been found in the Japanese and the Filipinos. As compared with the Japanese, the Taiwanese have a higher frequency of ORM1*1, and a significantly lower frequency of ORM1*2.1. 相似文献