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1.
Thanika Promchai Pawinee Janhom Wisanu Maneerat Roonglawan Rattanajak Sumalee Kamchonwongpaisan Stephen G. Pyne 《Natural product research》2020,34(10):1394-1398
AbstractA new 2-arylbenzofuran, spathobenzofuran (1), together with ten known compounds including a 2-arylbenzofuran, three pterocarpans and six isoflavones were isolated from the acetone crude extract of the stems of Spatholobus parviflorus. All compounds were characterised by spectroscopic methods. Compound 4 was active (MIC 8?µg/mL) against Gram-negative Pseudomonas aeruginosa TISTR 781 while compound 2 had modest activity against Gram-positive Staphylococcus aureus TISTR 1466 with a MIC value of 16?µg/mL. All isolated compounds showed no cytotoxicity against Vero and KB cells. 相似文献
2.
Leaves of Blumea balsamifera (Lin.) DC. are used in traditional Thai and Chinese medicine for the treatment of septic wounds and other infections. In this study, the essential oil, hexane, dichloromethane and methanol extracts of these leaves were evaluated for antibacterial and antifungal activities using the disc diffusion assay and agar microdilution method. The essential oil was the most potent, with a minimum inhibitory concentration (MIC) of 150?μg?mL?1 against Bacillus cereus and an MIC of 1.2?mg?mL?1 against Staphylococcus aureus and Candida albicans. Activity was also detected from the hexane extract against Enterobacter cloacae and S. aureus. Minimum bactericidal and fungicidal concentrations were typically equal to or two-fold higher than the MICs for both extracts, indicating microbicidal activity. The present data show that B. balsamifera extracts have activity against various infectious and toxin-producing microorganisms. This plant's active constituents could potentially be developed for use in the treatment and/or prevention of microbial disease. 相似文献
3.
Maneerat Yodjun Aphichart Karnchanatat Polkit Sangvanich 《Applied biochemistry and biotechnology》2012,166(8):2037-2050
Ammonium sulphate cut protein extracts, and their pepsin hydrolysates, from the rhizomes of 15 plants in the Zingiberaceae
family were screened for their in vitro angiotensin I-converting enzyme inhibitory (ACEI) activity. The protein extract from
Zingiber ottensii had the highest ACEI activity (IC50 of 7.30 × 10−7 mg protein/mL) and was enriched for by SP Sepharose chromatography with five NaCl step gradients 0, 0.25, 0.50, 0.75 and
1 M NaCl collecting the corresponding five fractions. The highest ACEI activity was found in the F75 fraction, which appeared
to contain a single 20.7-kDa protein, suggesting enrichment to or near to homogeneity. The ACEI activity of the F75 fraction
was moderately thermostable (−20–60 °C), showed >80% activity across a broad pH range of 4–12 (optimal at pH 4–5) and appeared
as a competitive inhibitor of ACE (K
i of 9.1 × 10−5 mg protein/mL). For the pepsin hydrolysates, that from Zingiber cassumunar revealed the highest ACEI activity (IC50 of 0.38 ± 0.012 mg/mL), was enriched to a single active hexapeptide by RP-HPLC with a strong ACEI activity (IC50 of 0.011 ± 0.012 mg/mL) and acted as a competitive inhibitor of ACE (K
i of 1.25 × 10−6 mg protein/mL). 相似文献
4.
Chanika Saenge Chooklin Suppasil Maneerat Atipan Saimmai 《Applied biochemistry and biotechnology》2014,173(2):624-645
In this study, biosurfactant-producing bacteria was evaluated for biosurfactant production by using banana peel as a sole carbon source. From the 71 strains screened, Halobacteriaceae archaeon AS65 produced the highest biosurfactant activity. The highest biosurfactant production (5.30 g/l) was obtained when the cells were grown on a minimal salt medium containing 35 % (w/v) banana peel and 1 g/l commercial monosodium glutamate at 30 °C and 200 rpm after 54 h of cultivation. The biosurfactant obtained by extraction with ethyl acetate showed high surface tension reduction (25.5 mN/m), a small critical micelle concentration value (10 mg/l), thermal and pH stability with respect to surface tension reduction and emulsification activity, and a high level of salt tolerance. The biosurfactant obtained was confirmed as a lipopeptide by using a biochemical test FT-IR, NMR, and mass spectrometry. The crude biosurfactant showed a broad spectrum of antimicrobial activity and had the ability to emulsify oil, enhance PAHs solubility, and oil bioremediation. 相似文献
5.
Pongsak Noparat Suppasil Maneerat Atipan Saimmai 《Applied biochemistry and biotechnology》2014,172(8):3949-3963
This study aimed at investigating the application of biosurfactant from Sphingobacterium spiritivorum AS43 using molasses as a substrate and fertilizer to enhance the biodegradation of used lubricating oil (ULO). The cell surface hydrophobicity of bacteria, the emulsification activity, and the biodegradation efficiency of ULO were measured. The bacterial adhesion in the hydrocarbon test was used to denote the cell surface hydrophobicity of the used bacterial species. The results indicate a strong correlation between cell surface hydrophobicity, emulsification activity, and the degree of ULO biodegradation. The maximum degradation of ULO (62 %) was observed when either 1.5 % (w/v) of biosurfactant or fertilizer was added. The results also revealed that biosurfactants alone are capable of promoting biodegradation to a large extent without added fertilizer. The data indicate the potential for biosurfactant production by using low-cost substrate for application in the bioremediation of soils contaminated with petroleum hydrocarbons or oils. 相似文献
6.
Maneerat Ongwandee Glenn C. Morrison Xiaowen Guo Charles C. Chusuei 《Colloids and surfaces. A, Physicochemical and engineering aspects》2007,310(1-3):62-67
Sorptive interactions of volatile organic compounds (VOCs) with indoor surfaces play a major role in inhalation exposure to these species. Using ZrSiO4 and polyethylene (PE) to model mineral surfaces and carpeting, respectively, the adsorption behavior of gaseous trimethylamine (TMA) was examined under conditions of 80% relative humidity (RH) in N2 and in the presence of 1000 ppm CO2 or NH3. TMA adsorption and desorption behavior were studied using attenuated total reflection infrared (ATR-IR) and X-ray photoelectron (XPS) spectroscopies. Spectral data revealed that TMA adsorbed on both surfaces in a protonated state. Stronger adsorption was observed to occur on ZrSiO4. XPS scans indicate that the “dry” ZrSiO4 surface maintains OH groups available for bonding, supporting earlier research showing that partition coefficients increase as RH decreases. 相似文献
7.
Atipan Saimmai Onkamon Rukadee Theerawat Onlamool Vorasan Sobhon Suppasil Maneerat 《Applied biochemistry and biotechnology》2012,168(5):1003-1018
Microbial surface active compounds (SACs) were isolated from various environmental sources in Thailand. Isolates were screened for SACs production in different carbon sources (crude glycerol, commercial sugar, decanter, glucose, molasses, used palm oil, and used lubricating oil) by using drop-collapsing test and emulsification activity. Molasses produced the highest number of positive results (23 of 25 isolates). Twenty-one isolate strains produced emulsions with xylene, and 15 exhibited high emulsion-stabilizing capacity, maintaining more than 50?% of the original emulsion volume for 24?h, and six isolate strains reduced the growth medium surface tension to 40?mN/m. The phylogenetic position of these 25 isolates was evaluated by 16S rRNA gene sequence analysis. The production of microbial SACs was determined for strains representative of 16 different bacterial genera, in which ten genera (Blastococcus, Erysipelothrix, Humicoccus, Methylophilus, Microlunatus, Nevskia, Pectinatus, Rubrimonas, Selenomonas, and Stenotrophomonas) were firstly reported as SAC-producing strains. Overall, the new SAC-producing strains isolated in this study display promising features for the future development and use in economically efficient industrial-scale biotechnological processes. 相似文献
8.
Darin Phukingngam Orathai Chavalparit Dararat Somchai Maneerat Ongwandee 《Chemical Papers》2011,65(5):644-651
Biodiesel-processing factories employing the alkali-catalyzed transesterification process generate a large amount of wastewater
containing high amount of methanol, glycerol, and oil. As such, wastewater has high potential to produce biogas using anaerobic
treatment. The aim of this research was to investigate the performance of an anaerobic baffled reactor for organic removal
and biogas production from biodiesel wastewater. The effect of different organic loading rates, varying from 0.5 kg m−3 d−1 to 3.0 kg m−3 d−1 of chemical oxygen demand, was determined using three 22 L reactors, each comprising five separate compartments. Wastewater
was pretreated with chemical coagulants to partially remove oil prior to experimentation. Results show that the anaerobic
baffled reactor operated at 1.5 kg m−3 d−1 of chemical oxygen demand and ten days of hydraulic retention time provided the best removal efficiencies of 99 % of chemical
oxygen demand, 100 % of methanol, and 100 % of glycerol. Increasing the organic loading rate over 1.5 kg m−3 d−1 of chemical oxygen demand led to excessive accumulation of volatile fatty acids thereby making the pH drop to a value unfavorable
for methanogenesis. The biogas production rate was 12 L d−1 and the methane composition accounted for 64–74 %. Phase-separated characteristics revealed that the highest chemical oxygen
demand removal percentage was achieved in the first compartment and the removal efficiency gradually decreased longitudinally.
A scanning electron microscopic study indicated that the most predominant group of microorganisms residing on the external
surface of the granular sludge was Methanosarcina. 相似文献
9.
Virayu Suthiphasilp Wisanu Maneerat Raymond J. Andersen Stephen G. Pyne Chatchai Muanprasat Sawinee Seemakhan 《Natural product research》2019,33(20):2945-2950
A phytochemical investigation of the fruit and root extracts of Micromelum integerrimum resulted in the isolation and identification of a new compound, integerravone (1), together with 23 known compounds (2–24). Their structures were characterized by spectroscopic methods as well as comparisons made from the literature. Compounds 2, 3–15, 17–18 and 20–23 were evaluated for their cytotoxicities against the colon cancer cell line, HCT116. All of them were inactive at 50?µM. Most of the phenolic compounds were evaluated for their antioxidant activity using the DPPH assay. Compounds 14 and 22–24 showed antioxidant activity with IC50 values ranging from 24.83-135.05?µM. 相似文献
10.
Two types of biosurfactant (BS)-producing bacteria, Bacillus licheniformis TR7 and Bacillus subtilis SA9, were isolated from mangrove sediment in the south of Thailand. The BS production was done by using only molasses as
a whole medium for growth and production. Under optimized conditions, the yields of TR7 and SA9 BS were found to be 3.30 and
3.78 g/l, respectively. It could reduce the surface tension of pure water to 28.5 and 29.5 mN/m, with the critical micelle
concentrations of about 10 and 30 mg/l, respectively. Good thermal, pH, and salt stability were exhibited. Both BSs could
recover oil more effectively than the two synthetic surfactants. In addition, TR7 and SA9 BS could enhance the solubility
of polyaromatic hydrocarbons (PAHs). Thus, these BSs have the potential for the removal of oil and PAHs from the combined
contaminated environment and facilitate its bioremediation. These studies indicate that molasses, as a renewable, relatively
inexpensive and easily available resource, can be used for important biotechnological processes. 相似文献