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Detection of p53 Gene by Using Genomagnetic Assay Combined with Carbon Nanotube Modified Disposable Sensor Technology 下载免费PDF全文
Electrochemical monitoring of DNA hybridization related to p53 gene sequence was investigated using genomagnetic assay combined with single walled carbon nanotube (SWCNT) modified pencil graphite electrodes (PGEs). The hybridization was performed either at magnetic beads (MB) surface or in solution. The enhanced guanine signal was obtained using SWCNT‐PGEs compared to one obtained by unmodified PGEs. The selectivity of genomagnetic assay was tested under optimum conditions. The DLs were calculated as 0.88 µM and 0.11 µM for hybridization performed at MB surface and solution, respectively. This selective, practical and cost effective genomagnetic assay combined with SWCNT‐PGEs is reported herein for the first time. 相似文献
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Jana Balintová Medard Plucnara Pavlína Vidláková Dr. Radek Pohl Dr. Luděk Havran Prof. Dr. Miroslav Fojta Prof. Dr. Michal Hocek 《Chemistry (Weinheim an der Bergstrasse, Germany)》2013,19(38):12720-12731
Benzofurazane has been attached to nucleosides and dNTPs, either directly or through an acetylene linker, as a new redox label for electrochemical analysis of nucleotide sequences. Primer extension incorporation of the benzofurazane‐modified dNTPs by polymerases has been developed for the construction of labeled oligonucleotide probes. In combination with nitrophenyl and aminophenyl labels, we have successfully developed a three‐potential coding of DNA bases and have explored the relevant electrochemical potentials. The combination of benzofurazane and nitrophenyl reducible labels has proved to be excellent for ratiometric analysis of nucleotide sequences and is suitable for bioanalytical applications. 相似文献
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Crew scheduling problems at the planning level are typically solved in two steps: first, creating working patterns, and then assigning these to individual crew. The first step is solved with a set covering model, and the second with a set-partitioning model. At the operational level, the (re) planning period is considerably smaller than during the strategic planning phase. We integrate both models to solve time critical crew recovery problems arising on the day of operations. We describe how pairing construction and pairing assignment are done in a single step, and provide solution techniques based on simple tree search and more sophisticated column generation and shortest-path algorithms. 相似文献
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Hároníková Lucia Špaček Jan Plucnara Medard Horáková Petra Pivoňková Hana Havran Luděk Erdem Arzum Fojta Miroslav 《Monatshefte für Chemie / Chemical Monthly》2015,146(5):849-855
Monatshefte für Chemie - Chemical Monthly - In this report, we present a simple electrochemical detection protocol for the detection of specific PCR-amplified DNA fragments, based on... 相似文献
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Burghardt S Hirsch A Medard N Kachfhe RA Ausseré D Valignat MP Gallani JL 《Langmuir : the ACS journal of surfaces and colloids》2005,21(16):7540-7544
We report the formation of highly stable Langmuir-Blodgett (LB) organic steps made with a hexa-adduct fullerene-based amphiphile. This amphiphile forms films of excellent quality, with a very low roughness, that are structurally stable: X-ray reflectivity spectra recorded on fresh and 12-month-old samples are undiscernible. Such a behavior contrasts with that of more traditional amphiphiles, which are unfortunately well-known for their instability in time. The stability of the films stems, among others, from the spheroidal shape of the constitutive molecules. These experiments show that it is possible to circumvent the major drawback of LB films and to prepare materials more suited for applications. We show that the LB film prepared with this fullerene derivative can successfully be used as thickness gauges for atomic force microscopy or light microscopy studies. 相似文献
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Summary The electronic and vibrational spectra of NiII and PdII complexes with thiobenzamide, L, are discussed. L acts as a sulphur donor ligand. The PdII compounds and (NiL4)(ClO4)2 are square planar. PdL2Cl2 has acis-structure, while PdL2X2 (X=Br or I) istrans; NiL4Cl2 istrans-octahedral. The i.r. bands due to(M.S) and(MX) have been assigned. The influence of the anions on the properties of the complexes, both in solution and in the solid state, is discussed. 相似文献
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