Affinity interactions between phenylboronic acid-carrying self-assembled monolayers and flavin adenine dinucleotide or horseradish peroxidase

A method is provided for the recognition of glycated molecules based on their binding affinities to boronate-carrying monolayers. The affinity interaction of flavin adenine dinucleotide (FAD) and horseradish peroxidase (HRP) with phenylboronic acid monolayers on gold was investigated by using voltammetric and microgravimetric methods. Conjugates of 3-aminophenylboronic acid and 3,3'-dithiodipropionic acid di(N-hydroxysuccinimide ester) or 11-mercaptoundecanoic acid were prepared and self-assembled on gold surfaces to generate monolayers. FAD is bound to this modified surface and recognized by a pair of redox peaks with a formal potential of -0.433 V in a 0.1 M phosphate buffer solution, pH 6.5. Upon addition of a sugar to the buffer, the bound FAD could be replaced, indicating that the binding is reversible. Voltammetric, mass measurements, and photometric activity assays show that the HRP can also be bound to the interface. This binding is reversible, and HRP can be replaced by sorbitol or removed in acidic solution. The effects of pH, incubation time, and concentration of H(2)O(2) were studied by comparing the catalytic reduction of H(2)O(2) in the presence of the electron-donor thionine. The catalytic current of the HRP-loaded electrode was proportional to HRP concentrations in the incubation solution in the range between 5 microg mL(-1) and 0.1 mg mL(-1) with a linear slope of 3.34 microA mL mg(-1) and a correlation coefficient of 0.9945.     

Gas-chromatographische analyse der trimethylsilyl-derivate phenolischer Verbindungen

Zusammenfassung Einfache Phenole, Phenolcarbonsäuren, Hydroxy- und Cumarine, Catechine, Anthocyanidine, Flavonole, Flavanone, Flavone und Flavanonole werden problemlos silyliert und als Trimethylsilyl (TMS)-Derivate gaschromatographisch quantitativ bestimmt. Die TMS-Derivate phenolischer Glycoside (Anthocyane) sind thermolabil. Ihre quantitative gas-chromatographische Analyse gelang in Allglassystemen bei einer Verdampfer-temperatur von 280°C und einem N₂-Durchfluß von 60–100 cm³/min. Nach Überprüfung von 14 verschiedenen Lösungsmittel-Silylierungsreagens-Kombinationen bzw. Silylierungsmitteln alleine derivatisierten wir mit N, N-Dimethylformamid (DMF): Hexamethyldisilazan (HMDS)=11 als universellem Silylierungsmittel für phenolische Verbindungen. Aglycone liefern bei Verwendung von Acetonitril anstelle von DMF als Lösungsmittel etwa 3mal höhere Silylierungsausbeuten.

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Analysis of phenolic compounds as trimethylsilyl derivatives by gas-liquid chromatography

Summary Simple phenols, phenolcarboxylic acids, hydroxy coumarins and coumarines, catechins, anthoxyanidins, flavonols, flavanones, flavones and flavanonols are silylated without difficulty and determined quantitatively as trimethylsilyl (TMS) derivates by gas-liquid chromatography. The TMS derivatives of phenolic glycosides (anthocyanins) however are quite thermolabile. Their quantitative gas-chromatographic analysis was developed in all-glass systems with an oven temperature of 280°C and a nitrogen flow rate of 60–100 cm³/min. After a study of 14 different combinations of solvents with silylation reagents and of silylation reagents alone we used N, N-dimethylformamide (DMF) with hexamethyl-disilazane (HMDS) 11, which is a universal silylation agent for phenolic compounds. Silylation of aglycones dissolved in acetonitrile instead of DMF leads to yields about three times higher.

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Auszug aus der Dissertation von G. Leupold, TU-München 1974. Die Arbeiten wurden durch den Forschungskreis der Ernährungsindustrie unterstützt.     

The impact of chirally odd condensates on the rho meson

Based on QCD sum rules we explore the consequences of a scenario for the ρ meson, where the chiral symmetry breaking condensates are set to zero whereas the chirally symmetric condensates remain at their vacuum values. This clean-cut scenario causes a lowering of the ρ spectral moment by about 120 MeV. The complementarity of mass shift and broadening is discussed. A simple parametrization of the ρ spectral function leads to a width of about 280 MeV if no shift of the peak position is assumed.     

Magnetism under high pressure studied by 57Fe and 151Eu nuclear scattering of synchrotron radiation

The nuclear forward scattering (NFS) of synchrotron radiation is especially suited for probing magnetism at very high pressure, here in the Mbar range, by the nuclear resonances of ⁵⁷Fe and ¹⁵¹Eu. We report on high pressure (h.p.) NFS studies with the 14.4 keV transition of ⁵7Fe on magnetic RFe₂ Laves phases of cubic C15 structure (YFe₂, GdFe₂) and hexagonal C14 structure (ScFe₂, TiFe₂) at pressures up to 100 GPa (=1 Mbar). We present also h.p. NFS studies performed with the 21.5 keV resonance of ¹⁵¹Eu, probing the magnetism in the CsCl-type h.p. phase of EuTe. This revised version was published online in August 2006 with corrections to the Cover Date.     

Automatic reduction and evaluation of infrared and Raman spectra

Summary The automatic interpretation of digital recorded infrared and Raman spectra is described. The procedure starts with the automatic correction for an approximated base line. In the case of Raman spectra the spectral response of the spectrometer is corrected, too. Subsequently, the spectra are reduced to band lists, conserving the position, intensity and half-widths of the bands. These band lists are the input in an interpretation algorithm for the combined evaluation of infrared and Raman spectra by testing presence or absence of characteristic bands or groups of bands. The algorithm was tested with 300 substances. A balance of the interpretation results shows that the abscence of structural elements is found more safely than the presence. The algorithm may be modified for special purposes: evaluation of infrared or Raman spectra alone or application to special classes of substances.

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Automatische Reduktion und Auswertung von IR- und Raman-SpektrenI. Interpretation charakteristischer Banden

Zusammenfassung Ziel dieser Arbeit ist die automatische Interpretation von Infrarot- und Raman-Spektren mit Hilfe charakteristischer Banden. Die Bearbeitung der Spektren beginnt mit der Korrektur der digital registrierten Spektren auf eine angenÄherte Grundlinie. Bei Raman-Spektren wird zusÄtzlich die spektrale Empfindlichkeit des Spektrometers korrigiert. Die Spektren werden auf Bandenlisten reduziert, in denen die Lage, IntensitÄt und Halbwertsbreite der einzelnen Banden enthalten sind. Diese Bandenlisten dienen als Eingabe in einen Interpretationsalgorithmus mit dem durch Prüfung der Lage und IntensitÄt einzelner charakteristischer Banden und Bandengruppen auf Anwesenheit oder Abwesenheit von Strukturmerkmalen geschlossen wird. Der Algorithmus wurde an 300 Substanzen getestet. Die Aussage über das Fehlen bestimmter Gruppen ist sicherer als die über deren Vorhandensein. Die Struktur des Auswertealgorithmus erlaubt ein einfaches Anpassen an verschiedene Anwendungsgebiete: Auswertung von Infrarot-oder Raman-Spektrum allein, bzw. Anwendung bei speziellen Substanzklassen.

     

Chromofields of strings and baryons

We calculate color electric fields of quark/antiquark ( ) and 3-quark (qqq) systems within the Chromodielectric Model (CDM). We explicitly evaluate the string tension of flux tubes in the -system and analyze their profile. To reproduce results of lattice calculations we use a bag pressure from which an effective strong-coupling constant follows. With these parameters we get a Y-shaped configuration for large qqq-systems.Received: 30 September 2002, Published online: 22 October 2003PACS: 11.10.Lm Field theory: Nonlinear or nonlocal theories and models - 11.15.Kc Gauge field theories: Classical and semiclassical techniques - 12.39.Ba Phenomenological quark models: Bag model     

Magnetic Properties of Monocrystal 57Fe/V Multilayers Investigated by CEMS,Nuclear Resonance Reflectivity in the Time Domain and Polarized Neutron Scattering

Hyperfine Interactions - We have performed a depth selective investigation of monocrystalline samples with composition [57Fe(10 ML)/V(5 ML)]20 and [57Fe(7 ML)/V(10 ML)]29 by means of synchrotron...     

Relaxation as seen by nuclei: Comparison between conventional Mössbauer spectra and nuclear resonant scattering of synchrotron radiation

The mechanism of spin‐lattice relaxation has been investigated in the “picket‐fence” porphyrin [Fe(CH₃COO)(TP_pivP)]^-, a high‐spin iron(II) complex with unusual large quadrupole splitting of 4.25 mm s^-1, by conventional Mössbauer spectroscopy as well as by nuclear resonant forward scattering (NFS). Superparamagnetism with a blocking temperature of about 8 K is observable by both methods in the spectra of bacterioferritin from S. olivaceus. From these two examples general conclusions about the merits of both methods can be drawn.