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Applied Biochemistry and Biotechnology - A novel, anaerobic fermentation process is developed to produce calcium magnesium acetate (CMA) from cheese whey. CMA can be used as a noncorrosive road... 相似文献
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Bai Y Koh CG Boreman M Juang YJ Tang IC Lee LJ Yang ST 《Langmuir : the ACS journal of surfaces and colloids》2006,22(22):9458-9467
A novel surface treatment method using poly(ethyleneimine) (PEI), an amine-bearing polymer, was developed to enhance antibody binding on the poly(methyl methacrylate) (PMMA) microfluidic immunoassay device. By treating the PMMA surface of the microchannel on the microfluidic device with PEI, 10 times more active antibodies can be bound to the microchannel surface as compared to those without treatment or treated with the small amine-bearing molecule, hexamethylenediamine (HMD). Consequently, PEI surface modification greatly improved the immunoassay performance of the microfluidic device, making it more sensitive and reliable in the detection of IgG. The improvement can be attributed to the spacer effect as well as the functional amine groups provided by the polymeric PEI molecules. Due to the smaller dimensions (140x125 microm) of the microchannel, the time required for antibody diffusion and adsorption onto the microchannel surface was reduced to only several minutes, which was 10 times faster than the similar process carried out in 96-well plates. The microchip also had a wider detection dynamic range, from 5 to 1000 ng/mL, as compared to that of the microtiter plate (from 2 to 100 ng/mL). With the PEI surface modification, PMMA-based microchips can be effectively used for enzyme linked immunosorbent assays (ELISA) with a similar detection limit, but much less reagent consumption and shorter assay time as compared to the conventional 96-well plate. 相似文献
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Mana-Hox, an analog of beta-carbolines with anticancer activity, induces aberrant mitosis and delays mitotic exit. However, the cellular target is not known. In this study, we visualized the intracellular localization of Mana-Hox. Mana-Hox rapidly penetrated into cells (within 1 min) and concentrated on disorganized metaphase chromosomes after 13 hr of exposure. We demonstrated that Mana-Hox is a noncovalent DNA binder that can interact with DNA through intercalation and/or through minor groove binding. Furthermore, Mana-Hox also inhibits topoisomerase II relaxation activity in vitro, suggesting that Mana-Hox could perturb mitotic chromosome decatenation. Overall, Mana-Hox binding to DNA plays a critical role in the induction of aberrant mitosis and contributes to its anticancer activity. 相似文献
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Douglass P. Taber Carlton Campbell Bruce P. Gunn I-Ching Chiu 《Tetrahedron letters》1981,22(51):5141-5144
Nonsynchronous bond formation in the transition state of the intramolecular Diels-Alder reaction is suggested to be an important determinant of the stereochemical outcome of the cyclization. 相似文献
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Lan Chun Tu Chien-Shu Chen I-Ching Hsiao Ji-Wang Chern Chi-Hung Lin Ya-Ching Shen Sheau Farn Yeh 《Chemistry & biology》2005,12(12):7613-1324
Mana-Hox, an analog of beta-carbolines with anticancer activity, induces aberrant mitosis and delays mitotic exit. However, the cellular target is not known. In this study, we visualized the intracellular localization of Mana-Hox. Mana-Hox rapidly penetrated into cells (within 1 min) and concentrated on disorganized metaphase chromosomes after 13 hr of exposure. We demonstrated that Mana-Hox is a noncovalent DNA binder that can interact with DNA through intercalation and/or through minor groove binding. Furthermore, Mana-Hox also inhibits topoisomerase II relaxation activity in vitro, suggesting that Mana-Hox could perturb mitotic chromosome decatenation. Overall, Mana-Hox binding to DNA plays a critical role in the induction of aberrant mitosis and contributes to its anticancer activity. 相似文献
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I-Ching Yang Chi-Long Lin Irina Radinschi 《International Journal of Theoretical Physics》2009,48(1):248-255
According to the Einstein, Weinberg, and M?ller energy-momentum complexes, we evaluate the energy distribution of the singularity-free
solution of the Einstein field equations coupled to a suitable nonlinear electrodynamics suggested by Ayón-Beato and García.
The results show that the energy associated with the definitions of Einstein and Weinberg are the same, but M?ller not. Using
the power series expansion, we find out that the first two terms in the expression are the same as the energy distributions
of the Reissner-Nordstr?m solution, and the third term could be used to survey the factualness between numerous solutions
of the Einstein field equations coupled to a nonlinear electrodynamics. 相似文献
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