Substrate specificity analysis of protein kinase complex Dbf2-Mob1 by peptide library and proteome array screening

Background  

The mitotic exit network (MEN) is a group of proteins that form a signaling cascade that is essential for cells to exit mitosis in Saccharomyces cerevisiae. The MEN has also been implicated in playing a role in cytokinesis. Two components of this signaling pathway are the protein kinase Dbf2 and its binding partner essential for its kinase activity, Mob1. The components of MEN that act upstream of Dbf2-Mob1 have been characterized, but physiological substrates for Dbf2-Mob1 have yet to be identified.     

Quantitative measurements of inorganic and methyl arsenicals by gas-liquid chromatography

Procedures are described for determinations of inorganic and methylated arsenicals in urine and water by gas—liquid chromatography of the corresponding diethyldithiocarbamate complexes. Silanized 5 % OV-17 on Anakrom AS as packing is used in an instrument equipped with electron-capture detection (³H foil). Samples of water or urine are treated to generate the iodides of inorganic, mono- and dimethylarsenic and these are treated with diethyldithiocarbamate, followed by isolation into various organic solvents. Recovery and precision s_r data for the analysis of the arsenicals in urine include: inorganic (1.0 p.p.m.), 82.6%, ± 7.7; monomethylarsenic (0.5 p.p.m.), 91.5 %, ± 7.0; dimethylarsenic (0.5 p.p.m.), quantitative, ± 4.0.

     

The determination of boron in solution to sub-p.p.b. concentrations by hollow-cathode emission

The determination of boron in solutions to sub-p.p.b. concentrations by hollow-cathode emission is demonstrated. The effects of cathode material, clean-up procedure, fill gas, and tube current were investigated to determine the conditions yielding optimal sensitivity. The boron atomic line emission is 200 times as intense as boron oxide band emission.     

Determination of traces of gold by hollow-cathode emission

The utility of a commercially available demountable hollow-cathode tube for emission spectrometric trace element analysis of solutions is demonstrated. The effects of cathode material, clean-up procedure, filler gas, and tube current were investigated to determine the conditions resulting in a satisfactory compromise between sensitivity and stability. Solutions of gold as myochrysine and as the metal dissolved in aqua regia were analyzed to show application of the commercial tube.     

Steady streaming around a spherical drop displaced from the velocity antinode in an acoustic levitation field

The steady (acoustic) streaming associated with a sphericaldrop displaced from the velocity antinode of a standing waveis studied. The ratio of the particle size to the acoustic wavelengthis treated as small but non-zero, and the solution is developedin the form of a two-term expansion in terms of the correspondingsmallness parameter. The drop viscosity is assumed to be muchhigher than that of the surrounding fluid, which is the casefor a drop in a gas medium. There are essentially three distinctregions where the steady streaming flow is analysed: insidethe drop (internal circulation), in the Stokes shear-wave layerat the surface on the gas side, and the gas outside the Stokeslayer (the outer streaming region). Solutions for the internalcirculation and the outer streaming are obtained in the limitof small Reynolds number. Despite the gas-to-liquid viscosity ratio being small, the outerstreaming may be dramatically affected by the fact that thesphere is liquid as opposed to solid. The parameter that measuresthe effect of liquidity is essentially the viscosity ratio dividedby the relative (to the particle size) thickness of the Stokeslayer. The case of a solid sphere is recovered by letting thisparameter go to zero.     

Ueber die Bestimmung des Cadmiums

Ohne Zusammenfassung