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正Protein kinases have been recognized as dominant regulators of all aspects of tumor progression, including tumorigenesis,proliferation, invasion, and metastasis [1]. During the activation of protein kinases, the binding of ligands (e.g., epidermal growth factor, transforming growth factor-α, and amphiregulin) can cause dimerization, which promotes the 相似文献
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Dielectric spectra of 4A zeolite particles bulked in deionized water (4A/W) or cyclohexane (4A/C) were measured respectively under different temperatures, and two dielectric relaxations were found in both of 4A/W and 4A/C. Because of existence of water molecules in pores, both low and high frequency relaxation times (τLFR and τHFR) of 4A/Wdecreasedmore sharply than 4A/C with increasing temperature. According to the temperature dependence of τLFR and τHFR, the activation energies of Na+ interacting with framework under hydrated and dehydrated environments were calculated respectively, and the original properties of Na+ in dehydrated zeolite were obtained by analyzing the dielectric properties of 4A/C. Furthermore, a transient state of Na+ motion with temperature was found in 4A/C system. 相似文献
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集成核酸提取的实时荧光PCR微全分析系统将核酸提取、PCR扩增与实时荧光检测进行整合,在同一块微流控芯片上实现了核酸分析过程的全自动和全封闭,具有试剂用量少、分析速度快、操作简便等优点。本研究采用微机械加工技术制作集成核酸提取微流控芯片的阳极模,使用组合模具法和注塑法制作具有3D通道的PDMS基片,与玻璃基底通过等离子体键合封装成集成核酸提取芯片。构建了由微流体速度可调节(0~10 mL/min)的驱动控制装置、温控精度可达0.1℃的TEC温控平台、CCD检测功能模块等组成的微全分析系统。以人类血液裂解液为样品,采用硅胶膜进行芯片上核酸提取。系统根据设置好的时序自动执行,以2 mL/min的流体驱动速度完成20μL裂解液上样、清洗;以1 mL/min的流体驱动速度完成DNA洗脱,抽取PCR试剂与之混合注入到反应腔。提取的基因组DNA以链上内参基因GAPDH为检测对象,并以传统手工提取为对照,在该系统平台上进行PCR扩增和熔解曲线分析实验。片上PCR扩增结果显示,扩增曲线明显,Ct值分别为25.3和26.9。扩增产物进行熔解曲线分析得到的熔解温度一致,均为89.9℃。结果表明,此系统能够自动化、全封闭的在微流控芯片上完成核酸提取、PCR扩增与实时定量分析。 相似文献
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This paper analyses a three-cavity frequency-quadrupling
terahertz gyroklystron with successive frequency-doubling in each
cavity with self-consistent nonlinear theory. The beam--wave
interaction efficiency and the electron bunching process are
studied. The variation of output efficiency with the length of drift
tubes and output power and the variation of Ohmic loss with the length of
output cavity are considered. Numerical simulations predict an
optimal output efficiency of 1.8%, a power output of more than
2~kW and a gain of 33~dB after taking into account Ohmic losses when
the frequency-quadrupling gyroklystron, driven by a 40-kV, 3-A
electron beam and 1 Watt input power, operates at 225~GHz. 相似文献
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This paper considers the frequency-quadrupling three-cavity
gyroklystrons with successive frequency-doubling in each cavity. The
cavities of 225~GHz frequency-quadrupling gyroklystron are designed
with the scattering matrices method and the possible operating mode
are discussed. With the point-gap theory, the starting currents of
the possible operating modes and the potential parasitic modes in the
output cavity are calculated. The optimal operating mode is proposed
under consideration of the mode competition and the power capacity
of the cavity. 相似文献
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