A high performance liquid chromatographic assay for AMP-deaminase activity in the erythrocytes of healthy subjects and patients with inherited purine disorders

A novel method for measuring AMP-deaminase activity in human erythrocytes is presented, based on the determination of the reaction product, IMP, using high performance liquid chromatography. IMP formation was found to be proportional both to the incubation time and the amount of haemolysate over a wide range. The minimal detectable AMP-deaminase activity was more than 1000 times lower than the mean activity found in healthy controls (1083 nmol/h/mg Hb). No marked difference of activity was found in the patients with the following inherited purine disorders: familial juvenile gouty nephropathy and deficiencies of adenosine deaminase, hypoxanthine-guanine phosphoribosyltransferase or adenine phosphoribosyltransferase. The activity in the erythrocytes of patients with chronic renal failure was also similar to controls. The existence of subjects with low erythrocyte AMP-deaminase activity in the population has been confirmed.     

Evaluation of Carboxen carbon molecular sieves for trapping replacement chlorofluorocarbons

A method has been developed for trapping and preconcentrating the very volatile replacement chlorofluorocarbons (hydrofluorocarbons and hydrochlorofluorocarbons) using microtraps filled with Carboxen, a carbon molecular sieve type material, without the need for extensive cryotrapping using liquid nitrogen. We present here the adsorption characteristics of four Carboxen materials, Carboxen 569, 1000, 1001, and 1002, used to trap a range of replacement chlorofluorocarbons varying in boiling point from −48.4 to −9.8°C. The application of these traps for the automated analysis of trace gases in atmospheric and environmental chemistry could prove extremely useful.     

Synthesis of the insect antifeedant CDE molecular fragment of 12-ketoepoxyazadiradione and related compounds

A diastereoselective synthesis of the model insect antifeedant 29 a CDE molecular fragment of 12-ketoepoxyazadiradione has been achieved in ten steps from indenone 9 in 44% overall yield. Several of the compounds obtained along the synthesis related to model compound 29 show significant antifeedant activity against Spodoptera littoralis and Spodoptera frugiperda.     

Synthesis of some phenanthridone derivatives

The alkylation of phenanthridone and some nuclear-substituted phenanthridones using sodium hydride in DMF/benzene is reported. Various 5-cyanoalkylphenanthridones were converted to further derivatives by either hydrolysis or reduction.     

Publications of Cornelius O. Horgan

Bibliography

Publications of Cornelius O. Horgan