全文获取类型
收费全文 | 384篇 |
免费 | 16篇 |
国内免费 | 1篇 |
专业分类
化学 | 299篇 |
晶体学 | 3篇 |
力学 | 3篇 |
数学 | 22篇 |
物理学 | 74篇 |
出版年
2023年 | 2篇 |
2022年 | 3篇 |
2021年 | 3篇 |
2019年 | 4篇 |
2018年 | 7篇 |
2016年 | 9篇 |
2015年 | 7篇 |
2014年 | 8篇 |
2013年 | 25篇 |
2012年 | 14篇 |
2011年 | 16篇 |
2010年 | 11篇 |
2009年 | 9篇 |
2008年 | 19篇 |
2007年 | 20篇 |
2006年 | 22篇 |
2005年 | 21篇 |
2004年 | 21篇 |
2003年 | 17篇 |
2002年 | 12篇 |
2001年 | 10篇 |
2000年 | 11篇 |
1999年 | 2篇 |
1998年 | 7篇 |
1997年 | 4篇 |
1996年 | 4篇 |
1995年 | 3篇 |
1994年 | 5篇 |
1993年 | 4篇 |
1992年 | 6篇 |
1991年 | 5篇 |
1990年 | 5篇 |
1989年 | 7篇 |
1988年 | 5篇 |
1987年 | 4篇 |
1985年 | 11篇 |
1984年 | 7篇 |
1982年 | 6篇 |
1981年 | 9篇 |
1980年 | 3篇 |
1979年 | 7篇 |
1978年 | 4篇 |
1977年 | 3篇 |
1976年 | 1篇 |
1975年 | 4篇 |
1973年 | 7篇 |
1972年 | 2篇 |
1968年 | 1篇 |
1966年 | 1篇 |
1960年 | 1篇 |
排序方式: 共有401条查询结果,搜索用时 15 毫秒
1.
The convenient method for the synthesis of 1,3-oxathiolan-2-ones by the reaction of epoxides with elemental sulfur and carbon monoxide in the presence of catalytic amount of sodium hydride has been developed. 相似文献
2.
The effect of antimony concentration (C(Sb)/mass%) on the surface tension of molten silicon has been determined with the sessile drop method in the temperature range from 1693 to 1773 K and in the range of the oxygen partial pressure, Po(2), in an Ar atmosphere from 10(-23) to 10(-21) MPa. The results show that the surface tension of molten silicon decreases with increasing Sb concentration in the range of C(Sb)<0.9 mass%, which indicates positive adsorption of Sb in molten silicon and can be fairly described with the Szyszkowski's equation. The maximum decrease rate of surface tension is about 65 mN m(-1) (mass% C(Sb))(-1), and the temperature coefficient of surface tension, (partial differential sigma/ partial differential T)C(Sb), increases with increasing C(Sb). The evaporation of the systems was only observed between the melting points of antimony (904 K) and silicon (1683 K), and the surface tension presents no dependence on measuring time above the melting point of silicon. 相似文献
3.
Masanari KimuraRyutaro Mukai Naoko TanigawaShuji Tanaka Yoshinao Tamaru 《Tetrahedron》2003,59(39):7767-7777
Without prior activation of allyl alcohols, allylation of a variety of active methylene compounds with allyl alcohols proceeds smoothly at rt-50°C in the presence of catalytic amounts of Pd(OAc)2 (1-10 mol%), Et3B (30-240 mol%), a phosphine ligand (1-20 mol%), and a base (0 to 50-60 mol%). 相似文献
4.
Michio Horiike Gu Yuan Chul-Sa Kim Chisato Hirano Katsuhiko Shibuya 《Journal of mass spectrometry : JMS》1992,27(9):944-948
Fourteen isomers of chemically unmodified hexadecenol were analysed by two types of quadrupole mass spectrometer, coupled with a gas chromatograph, for the location of the double-bond position. A series of spectra were interpreted in terms of mass spectral patterns on a fuzzy classification, in which the intensity ratios of six diagnostic pairs of the predominant ions were preferred in devising similarity indices. The accuracy of the method in the location of double-bond position was confirmed by testing with other series of spectra. 相似文献
5.
6.
When preparing an affinity column and a biosensor, it is desirable to immobilize a unimolecular layer of pure protein on a matrix. In this work, we tried to immobilize a monoclonal antibody on a surface of a glass test-tube as a model, to confirm the stability of this ultra-thin layer by an enzyme immunoassay, and to estimate the thickness of the layer on a slide glass by Fourier transform infrared reflection spectrometry. A new test-tube was washed and dried. The tube was filled with 5% 3-aminopropyltriethoxysilane. The 3-aminopropylsilylated surface was treated with glutaraldehyde and 5.6.10(-2) mg/ml solution of a normal mouse monoclonal antibody. The Schiff base between glutaraldehyde and the antibody was further reduced with 7.9.10(-3)% NaBH4. The tube was washed with 0.05% Tween 20 to block non-specific binding. The antibody immobilized on the surface was measured by an enzyme immunoassay based on a reaction of anti-mouse immunoglobulin G labelled with alkaline phosphatase, with which p-nitrophenol was produced from p-nitrophenylphosphate as a substrate. Meanwhile, various amounts of the antibody were immobilized on slide glasses in the same manner. The antibody on each surface was measured by Fourier transform infrared reflection spectrometry. The antibody immobilized under the final conditions was detectable by the enzyme immunoassay, and stable at 4 degrees C for ten days. The antibody on the slide glass was a unimolecular layer, as judged from the Fourier transform infrared spectra referred to -CONH- band semiquantitatively. Thus, we found the optimal conditions for immobilizing an ultra-thin layer of the monoclonal antibody on the glass surface.(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
7.
Evaluation of the adsorption affinity of proteins to calcium hydroxyapatites by desorption and pre-adsorption methods 总被引:1,自引:0,他引:1
Kazuhiko Kandori Aya Fujiwara Megumi Mukai Akemi Yasukawa Tatsuo Ishikawa 《Colloids and surfaces. B, Biointerfaces》1998,11(6):313-320
The adsorption affinity of bovine serum albumin (BSA) and lysozyme (LSZ) to calcium hydroxyapatite (CaHAP) was evaluated by desorption and two step adsorption methods. These experiments were carried out at 15°C in a 1×10−4 mol dm−3 KCl solution of pH 6.0. BSA molecules were scarcely desorbed, exhibiting an irreversible adsorption of BSA, though LSZ slightly desorbed. This result supports our previous findings that LSZ adsorbs weakly onto phosphate ions exposed on ac or bc faces of CaHAP while BSA adsorbs strongly onto positively charged sites on ac or bc faces of CaHAP. The amount of adsorbed LSZ was markedly increased by the pre-adsorption of BSA, where LSZ was adsorbed onto BSA-covered CaHAP. On the other hand, the amount of adsorbed BSA was not changed by the pre-adsorption of LSZ. In both pre-adsorption systems it was confirmed by an HPLC method that no protein molecule pre-adsorbed was desorbed after the post-adsorption procedure. Therefore, it was interpreted that the enhancement of adsorption of positively charged LSZ is induced by an electrostatic attractive force through pre-adsorption of negatively charged BSA molecules with a high coverage. However, since the coverage of LSZ onto CaHAP is considerably low, no stimulation of BSA adsorption occurred on the LSZ-covered surface. The formation of double protein adsorbed layers consisting of pre- and post-adsorbed proteins was proposed. 相似文献
8.
Miyoji Hanaoka Hiroshi Yamagishi Mari Marutani Chisato Mukai 《Tetrahedron letters》1984,25(45):5169-5172
Antileukemic benzo[C]phenanthridine alkaloids, fagaronine (1a) and nitidine (1c) were synthesized from the corresponding protoberberines through C6-N bond fission and subsequent cyclization between C6 and C13 position of the protoberberines. 相似文献
9.
Determination of protein spots separated by two-dimensional polyacrylamide gel electrophoresis 总被引:1,自引:0,他引:1
A simple method for quantitating proteins in the spots on two-dimensional polyacrylamide gel electropherograms is described. The system consists in three steps: (1) O'Farrell's two-dimensional gel electrophoresis of the proteins to be analysed; (2) staining of the gels with Coomassie brilliant blue; and (3) determination of the area and integrated density of the stained spots by the Joyce Loebl Magiscan-1 image analysis system. The method can be used for the determination of proteins in the range 0.5-100 micrograms/cm2; the amount of protein involved in most spots detected by the staining method actually falls within this range. As the minimum spot diameter that can easily be handled by the method is about 2 mm, as much as 30 ng of protein in such a spot can be determined. The method can also be applied to autoradiograms. 相似文献
10.
New fan-shaped ionic liquids forming columnar liquid crystalline phases have been prepared to obtain one-dimensional ion-transporting materials. The ionic liquids consist of two incompatible parts: an imidazolium-based ionic part as an ion-conducting part and tris(alkyloxy)phenyl parts as insulating parts. Two compounds having octyl and dodecyl chains have been synthesized. Self-assembly of these materials leads to the formation of thermotropic hexagonal columnar liquid crystalline states at room temperature. Anisotropic one-dimensional ionic conductivities have been successfully measured by the cells having comb-shaped gold electrodes. The self-organized columns have been aligned macroscopically in two directions by shearing perpendicular and parallel to the electrodes. The ionic conductivities parallel to the column axis are higher than those perpendicular to the axis. The incorporation of lithium salts in these columnar materials leads to the enhancement of the ionic conductivities and their anisotropy. These materials would be useful for anisotropic transportation of ions at the nanometer level. 相似文献