The venom gland of queens of Apis mellifera (Hymenoptera, Apidae): morphology and secretory cycle

The venom gland of queens of Apis mellifera was examined through light and transmission electron microscopy and subjected to electrophoretic analyses. Virgin queens exhibited prismatic secretory cells containing large amounts of rough endoplasmic reticulum with dilated cisternae, open secretory spaces, numerous vacuoles and granules scattered in the cytoplasm, and spherical nuclei with numerous nucleoli. The secretion produced was non-refringent under polarized light and the electrophoretic analysis of glandular extracts revealed five main protein bands. In mated queens, the venom gland exhibited a high degree of degeneration. Its secretion was refringent under polarized light and one of the main bands was absent in the electrophoretic pattern obtained. The morphological aspects observed are in agreement with the function of this gland in queens, given that virgin queens use venom in battles for the dominance of the colony, a situation that occurs as soon as they emerge, while fertilized queens rarely use venom.     

Comparative effects of macro-sized aluminum oxide and aluminum oxide nanoparticles on erythrocyte hemolysis: influence of cell source,temperature, and size

Al₂O₃ is the most abundantly produced nanomaterial and has been used in diverse fields, including the medical, military, and industrial sectors. As there are concerns about the health effects of nanoparticles, it is important to understand how they interact with cells, and specifically with red blood cells. The hemolysis induced by three commercial nano-sized aluminum oxide particles (nanopowder 13 nm, nanopowder <50 nm, and nanowire 2–6 × 200–400 nm) was compared to aluminum oxide and has been studied on erythrocytes from humans, rats, and rabbits, in order to elucidate the mechanism of action and the influence of size and shape on hemolytic behavior. The concentrations inducing 50 % hemolysis (HC₅₀) were calculated for each compound studied. The most hemolytic aluminum oxide particles were of nanopowder 13, followed by nanowire and nanopowder 50. The addition of albumin to PBS induced a protective effect on hemolysis in all the nano-forms of Al₂O₃, but not on Al₂O₃. The drop in HC₅₀ correlated to a decrease in nanomaterial size, which was induced by a reduction of aggregation. Aluminum oxide nanoparticles are less hemolytic than other oxide nanoparticles and behave differently depending on the size and shape of the nanoparticles. The hemolytic behavior of aluminum oxide nanoparticles differs from that of aluminum oxide.     

Evaluating the performance of an inexpensive,commercially available,NFC-powered and smartphone controlled potentiostat for electrochemical sensing

To move electrochemical sensing technology from the laboratory into point-of-care (POC)-based settings, electrochemical platforms must be cost-effective, portable, and user-friendly. Here, we present the comparison of a near-field communication (NFC) potentiostat with a traditional potentiostat. The NFC potentiostat is roughly the size of a credit card and is wirelessly powered by a smartphone with a user-friendly application for electrochemical analysis. The proposed system is portable, affordable, and provides analytical capabilities comparable to traditional benchtop potentiostats. Different redox probes were investigated, including ferricyanide, ruthenium hexamine, and hydroquinone, highlighting the ability of the NFC potentiostat to measure a variety of target analytes. In addition, the NFC potentiostat can perform several electrochemical techniques, such as cyclic voltammetry, differential pulse voltammetry, chronoamperometry and square wave voltammetry. The combination of affordable screen-printed carbon electrodes (SPCE)s with the portable, inexpensive NFC potentiostat highlights this platform‘s promise towards POC electrochemical sensing applications, particularly for medical diagnostics in rural or low-income areas.     

The Coevolution of Galaxies and Supermassive Black Holes in the Near Universe

A fundamental role is attributed to supermassive black holes (SMBH), and the feedback they generate, in the evolution of galaxies. But theoretical models trying to reproduce the M_SMBH vs. sigma relation (between the SMBH mass and stellar velocity dispersion of the galaxy bulge) make broad assumptions about the physical processes involved. These assumptions are needed due to the scarcity of observational constraints on the relevant physical processes which occur when the SMBH is being fed via mass accretion in active galactic nuclei (AGN). In search for these constraints, our group—AGN integral field spectroscopy (AGNIFS)—has been mapping the gas kinematics as well as the stellar population properties of the inner few hundred parsecs of a sample of nearby AGN hosts. In this contribution, I report on results obtained so far which show gas inflows along nuclear spirals and compact disks in the inner tens to hundreds of pc in nearby AGN hosts which seem to be the sources of fuel to the AGN. As the inflow rates are much larger than the AGN accretion rate, the excess gas must be depleted via formation of new stars in the bulge. Indeed, in many cases, we find ～100 pc circumnuclear rings of recent star formation (ages ～10–500 Myr) that can be interpreted as a signature of coevolution of the host galaxy and its AGN. I also report on the mapping of outflows in ionized gas, which are ubiquitous in Seyfert galaxies, and discuss mass outflow rates and powers.     

Optimal impulse control for a multidimensional cash management system with generalized cost functions

We consider the optimal control of a multidimensional cash management system where the cash balances fluctuate as a homogeneous diffusion process in Rⁿ${\mathbb{R}}^n$. We formulate the model as an impulse control problem on an unbounded domain with unbounded cost functions. Under general assumptions we characterize the value function as a weak solution of a quasi-variational inequality in a weighted Sobolev space and we show the existence of an optimal policy. Moreover we prove the local uniform convergence of a finite element scheme to compute numerically the value function and the optimal cost. We compute the solution of the model in two-dimensions with linear and distance cost functions, showing what are the shapes of the optimal policies in these two simple cases. Finally our third numerical experiment computes the solution in the realistic case of the cash concentration of two bank accounts made by a centralized treasury.     

Development and validation of an HPLC-PDA method for the determination of myrsinoic acid B in the extracts of Rapanea ferruginea Mez

New, simple, rapid and precise HPLC-PDA method has been developed and validated for quantification of biomarker myrsinoic acid B in stem bark extracts of Rapanea ferruginea Mez. The method employs a Phenomenex C18 column (250 mm × 4.6 mm I.D., 5 μm) with acetonitrile:methanol:water (pH 2.6 with phosphoric acid) at 48:30:22 as mobile phase, at a flow rate of 0.7 mL min⁻¹ and photo diode array (PDA) detection at 270 nm. The validation data show that the method is specific, accurate, precise and robust. The method was linear, over a range of 5-100.0 μg mL⁻¹, with a limit of detection of 0.369 μg mL⁻¹ and limit of quantification of 1.233 μg mL⁻¹. The method has also shown consistent recoveries (average of 101.3% and 0.12% RSD) of the biomarker, with low intra and inter-day relative standard deviation (1.26% and 1.62%, respectively). The evaluated hydroethanolic extract and dry extract presented MAB values of 63.53 and 36.07 mg g⁻¹, respectively.     

Comprehensive analysis of glycated human serum albumin tryptic peptides by off-line liquid chromatography followed by MALDI analysis on a time-of-flight/curved field reflectron tandem mass spectrometer

Glycated peptides arising from in vivo digestion of glycated proteins, usually called advanced glycation end (AGE) product peptides, are biologically relevant compounds due to their reactivity towards circulating and tissue proteins. To investigate their structures, in vitro glycation of human serum albumin (HSA) has been performed and followed by enzymatic digestion. Using different MALDI based approaches the digestion products obtained have been compared with those arising from enzymatic digestion of the protein. Results obtained using 2,5-dihydroxybenzoic acid (DHB) indicate this as the most effective matrix, leading to an increase in the coverage of the glycated protein. Off-line microbore liquid chromatography prior to MALDI analysis reveals that 63% of the free amino groups amenable to glycation are modified. In addition, the same approach shows the co-presence of underivatised peptides. This indicates that, regardless of the high glucose concentration employed for HSA incubation, glycation does not go to completion. Tandem mass spectrometric data suggest that the collision induced dissociation of singly charged glycated peptides leads to specific fragmentation pathways related to the condensed glucose molecule. The specific neutral losses derived from the activated glycated peptides can be used as signature for establishing the occurrence of glycation processes.     

Temporal and morphological differences in post-embryonic differentiation of the mushroom bodies in the brain of workers, queens, and drones of Apis mellifera (Hymenoptera, Apidae)

The mushroom bodies are structures present in the insect brain described as centers for the neural basis of learning, memory, and other higher functions. Honeybees (Apis mellifera) are insects with a sophisticated system of spatial orientation and possess well-developed learning and memory capabilities, which are associated with neural and brain structures. Thus, the present study aimed to compare the mushroom bodies during post-embryonic development and in newly emerged males, workers, and queens using light and transmission electron microscopy to examine how differential morphological characteristics are established during development. Measurements of structures were also taken in several post-embryonic developmental phases in order to evaluate size differences during the process and in the adult organs. The results show that workers, queens, and males exhibit temporal and size differences during the post-embryonic development of mushroom bodies, probably as adaptations to differences in behavior complexity. The mushroom bodies of workers are precociously formed and are larger than those of queens and drones. Thus, workers have the largest mushroom bodies resulting from differential development during metamorphosis.     

Optimal impulse control on an unbounded domain with nonlinear cost functions

In this paper we consider the optimal impulse control of a system which evolves randomly in accordance with a homogeneous diffusion process in ℜ¹. Whenever the system is controlled a cost is incurred which has a fixed component and a component which increases with the magnitude of the control applied. In addition to these controlling costs there are holding or carrying costs which are a positive function of the state of the system. Our objective is to minimize the expected discounted value of all costs over an infinite planning horizon. Under general assumptions on the cost functions we show that the value function is a weak solution of a quasi-variational inequality and we deduce from this solution the existence of an optimal impulse policy. The computation of the value function is performed by means of the Finite Element Method on suitable truncated domains, whose convergence is discussed. Mathematics Subject Classification: 49J40, 60G40, 65N30     

Differences in mushroom bodies morphogenesis in workers,queens and drones of Apis mellifera: Neuroblasts proliferation and death

Apis mellifera is an interesting model to neurobiological studies. It has a relatively small brain that commands the complex learning and memory tasks demanded by the social organization. An A. mellifera colony is made up of a queen, thousands of workers and a varying number of drones. The latter are males, whereas the former are the two female castes. These three phenotypes differ in morphology, physiology and behavior, correlated with their respective functions in the society. Such differences include the morphology and architecture of their brains. To understand the processes generating such polymorphic brains we characterized the cell division and cell death dynamics which underlie the morphogenesis of the mushroom bodies, through several methods suitable for evidence the time and place of occurrence. Cell death was detected in mushroom bodies of last larval instar and mainly in black-eyed pupae. Cell division was observed in mushroom bodies, primarily at the start of metamorphosis, exhibiting temporal differences among workers, queens and males.