CHARACTERISTICS OF THE PRIMARY REACTION OF HYPOCRELLIN A PHOTOSENSITIZATION

The primary reaction of photosensitization of Hypocrellin A (HA) has been studied byusing techniques of ESR, spin-trapping and spin-counteraction. The experiments show thatHA is able to generate not only ~1O_2,but also O_2~-·OH and HA~- which are observed for thefirst time. The conversion of generating active oxygen into generating nonoxygen free rad-ical is confirmed as well. Based on the characteristics of the primary reaction which gene-rates these active substances (transient products), it is proposed that the photosensitized dam-age for the biological system by HA is probably related to not only ~1O_2 itself, but also amultiple effect from ~1O_2 as well as O_2~-,·OH and HA~+ free radical.     

光卟啉(YHPD)光敏作用机理的探讨

本文用近代ESR技术,探讨了YHPD光敏反应的特征,结果指出:(1)YHPD不仅有产生~1O_2的作用,而且还产生了自由基(O_2·OH及YHPD);(2)产生~1O_2的能力,YHPDBHPD,这表明,其一,YHPD并非单—~1O_2起作用,而且尚与自由基(O_2,·OH,YHPD)的作用有关;其二,YHPD的光敏损伤作用虽优于BHPD,但这与它们的~1O_2产额成负相关性,而却与O_2和·OH的产额成正相关性,由该特征判别,对YHPD的光敏损伤,起主要作用的瞬态活性质,看来是活性氧自由基(O_2,·OH),而不是~O_2。     

INQUIRY OF PHOTOSENSITIZATION MECHANISM OF YANGZHOU HEMATOPORPHYRIN DERIVATIVE(YHPD)

By the use of the advanced ESR technique and through comparing with BHPD, the characteristic of YHPD photosensitization is discussed in this paper in the respect of the primary process of photosensitization. The experiment results showed: (ⅰ) not only ~1O_2, but also free radicals(O_2· OH and YHPD)can be formed by the aid of YHPD; and (ⅱ) as to the ability of producing ~1O_2, YHPDBHPD. Two points are indicated: first, the photosensitized damage of YHPD is interrelated to not only ~1O_2, but also free radicals (O_2. OH and YHPD); second, although the photosensitized damage of YHPD is stronger than that of BHPD, yet the photosensitized damage is negatively correlated to the yield of ~1O_2 but positively correlated to those of O_2 and OH. Based on these two points, it is suggested that activated oxygen free radicals(O_2 and. OH) instead of ~1O_2 play the main role as instantaneously activated material in the photosensitized damage of YHPD.     

磺基水杨酸化学发光新体系的研究 Ⅰ.痕量钴的分析及反应机理的探讨

化学发光分析作为一种灵敏度高,线性响应范围宽的测试手段正在受到重视。国内关于化学发光分析的研究已有一良好的开端,但大都限于鲁米诺(Luminol),光泽精(Lu-cigeniv)等一些老体系的应用。近年来,关于新化学发光体系的分析研究工作已见报道。磺基水杨酸(SSA)在金属离子的配合物吸收光谱分析中常用作显色剂和掩蔽剂,它便宜而易得。我们发现SSA在碱性过氧化氢溶液中,在某些金属离子存在时,可产生较强的化学发光。一些金属离子对SSA-H_2O_2体系发光反应的催化作用是测定这些金属的基础。我们     

竹红菌甲素光敏作用原初反应的特征

为了从光生物方面判别竹红菌甲素(Hypocrellin A)的光敏作用,本文用电子自旋共振(ESR)、自旋捕捉和消自旋等方法,探讨了HA光敏作用原初反应的特征。不仅观测到它产生~1O_2的作用,还首次观察到(?),·OH和(?)的产生,并且证实,活性氧产生可转变为非氧自由基生成。根据产生这些活性物质(瞬态产物)的原初反应特征,可能表明,竹红菌甲素对生物系统的光敏损伤机制,不只包括~1O_2的作用,而且尚与(?),·OH和非氧自由基的多重作用有关。