造血干细胞与造血微环境辐射损伤的比较研究

小鼠经低剂量率(70拉德/天)γ线连续照射25天后,在继续观察的一年期间,可以见到造血干细胞和造血微环境在数量和功能上都发生了明显的改变。对照射小鼠移植同系小鼠骨髓细胞,有明显的促进造血功能恢复的作用,支持了造血干细胞在辐射损伤中的重要地位。     

低剂量率γ线连续照射下造血干细胞损伤性质的研究

小鼠在低剂量率γ线连续照射下,造血干细胞不仅在数量上大幅度减少,而且在功能上也发生了深刻的变化。随着累积照射剂量的增加,可以见到骨髓造血微环境也受到不同程度的破坏。由于造血干细胞和造血微环境同时受了害,这就加重了造血功能的破坏。从正常和经低剂量率γ线连续照射后30天的小鼠骨髓细胞,在800拉德照射受体小鼠脾脏中,或在体内扩散盒培养条件下CFU-S生长特性的比较中,可以进一步了解小鼠在低剂量率γ线连续照射下造血干细胞性能的改变,主要是细胞增殖能力或自我更新能力的降低。     

高效液相色谱法定量分析人胎肝细胞上清液及细胞裂解液中7-酮基胆固醇

用高效液相色谱法定量分析了７－酮基胆固醇在胎肝细胞上清液及细胞裂解液中的含量。色谱柱为μ－ＰｏｒａｓｉｌＳｉＯ２柱,流动相为正己烷∶异丙醇（９１∶９,Ｖ／Ｖ）。方法回收率高、色谱重复性好、分析速度快。     

STUDIES OF HAEMOPOIETIC STEM CELLS AND MICROENVIRONMENT IN CHRONIC IRRADIATED MICE

Mice were irradiated at a dose rate of 70 rad per day for 25 days. Changes in properties and functions of haemopoietic stem cells and microenvironment were observed through a period of 12 months after termination of continuous irradiation. It was shown that the radiation damage of haemopoietic stem cells played an important role in the radiation-induced damage of haemopoiesis. This was further supported by the fact that transplantation of syngeneic bone marrow cells immediately after termination of continuous irradiation at 70 rad per day for 25 days would greatly improve the haemopoietic function of the irradiated mice, including the total recovery of CFU-S and numbers in the bone marrow and cell counts in the peripheral blood.