人食管癌细胞与正常食管上皮细胞内微管的免疫荧光细胞化学研究

本文利用抗微管蛋白抗体间接免疫荧光细胞化学技术,观察到人食管癌ECa109细胞的微管分布与正常食管上皮细胞有明显差别。原代培养的人正常食管上皮细胞胞质内有间期的纤细的微管结构(CMTC)和分裂期的纺锤体微管。食管癌ECa109细胞在间期的胞质内缺乏微管,但分裂期的纺锤体微管与正常细胞相同。本文对癌细胞间期胞质缺乏微管的现象进行了讨论,认为可能涉及微管组装机制的缺陷。     

IMMUNOFLUORESCENT STUDIES ON MICROTUBULES OF NORMAL AND MALIGNANT HUMAN ESOPHAGEAL CELLS

Anti-tubulin antibody indirect immunofluorescenee microscopy is used in this report. Difference of intracellular microtubule fluorescent staining patterns between normal and malignant human esophageal cells has been observed.Primarily cultured human esophageal epithelial cells display an elaborate array of fluorescent cytoplasmic microtubule filaments, the cytoplasmic microtubule complex(CMTC), in interphase cells and mitotic spindle microtubules in mitotic cells. Malignant esophageal cells from human esophageal carcinoma, ECa109 cells, contain very few cytoplasmic microtubules in interphase cells, but there is no difference of mitotic spindle microtubule fluorescence between normal and malignant cells.The results presented in this report suggest that the diminution of interphase cytoplasmic mierotubules in tumor cells is probably due to the deficiency of microtubule organizing mechanism in interphase tumor cells.