色谱法分离纯化人绒毛膜促性腺激素的研究

本文采用Ｄ３５２０大孔吸附树脂吸附层析，ＤＥＡＥ—Ｃｅｌｌｕｌｏｓｅ２３和ＤＥＡＥ—ＳｅｐｈａｄｅｘＡ５０离子交换层析，ＳｅｐｈａｄｅｘＧ７５凝胶过滤等方法，从孕妇尿制得人绒毛膜促性腺激素，放免活性为８５００～９０００ＩＵ／ｍｇ，生物活性为６０００～６５００ＩＵ／ｍｇ，ＳＤＳ—ＰＡＧＥ出现α和β亚基两条谱带，热原合格，促鲶鱼排卵实验效果较好。     

人低密度脂蛋白~125Ⅰ固相标记──Iodogen法

本文报道了利用固相氧化剂１，３，４，６，—四氯—３ａ，６ａ，－二苯基甘脲（Ｉｏｄｏｇｅｎ）对人血清低密度脂蛋（ＬＤＬ）进行１２５Ⅰ标记的方法，结果表明：１２５Ｉ－ＬＤＬ的比放射性为１８５～５１８ｃｐｍ／ｎｇＬＤＬ，游离１２５Ⅰ含量在０．２７～１．７５％之间，脂质标记率＜１％，１２５Ｉ－ＬＤＬ浓度２５０～７５０μｇ／ｍｌ，１２５Ｉ－ＬＤＬ的生物活性经受体实验证实与天然脂蛋白一致．符合脂蛋白代谢研究的要求．本法具有反应条件温和，不易损伤脂蛋白，脂质标记率低．固相标记，不需要复杂设备等优点，为进行脂蛋白受体的研究提供了可靠的方法．     

Spiral piezoelectric transducer in torsional motion as low-frequency power harvester

A structure consisting of a spiral piezoelectric transducer and a concentrated mass is proposed as a low-frequency piezoelectric power harvester. A theoretical model is developed for the system from the theory of piezoelectricity. An analysis is performed to demonstrate the low-frequency nature of the system. Other basic characteristics of the power harvester including the output power, voltage, and efficiency are also calculated and examined.     

Nonlinear characteristics of circular-cylinder piezoelectric power harvester near resonance based on flow-induced flexural vibration mode

The nonlinear behaviors of a circular-cylinder piezoelectric power harvester （CCPPH） near resonance are analyzed based on the flow-induced flexural vibration mode. The geometrically-nonlinear effect of the cylinder is studied with considering the in-plane extension incidental to the large defection. The boundary electric charges generated from two deformation modes, flexure and in-plane extension, were distinguished with each other because the charge corresponding to the latter mode produces no contribution to the output current. Numerical results on output powers show that there are multi- valuedness and jump behaviors.     

离子交换剂在麦胚凝集素制备中的应用

本文采用D261、001×7、DEAE—Cellulose—32和CM—Cellulose—32离子交换剂进行柱层析,去除麦胚提取液中褐色、红色、黄色、绿色、黑色等色素物质及杂蛋白。所制备的麦胚凝集素(Wheat Germ agglutinin简称WGA,下同),凝血活性高于Sigma公司产品,对等体积2％人A型红血球最小凝集剂量是4μg/ml。WGA是一种同工凝集素,其等电点分别是pH5.9、pH6.2、pH6.8,经SDS—PAGE检测它们的表观分子量分别是15KD、18KD和36KD。