聚硅氧烷液晶作为毛细管色谱固定液的研究

]本文研制了聚硅氧烷液晶玻璃毛细管柱,考察了其色谱性能,结果表明这种柱柱效高,对多环芳烃选择性好,热稳定性好,使用温度范围宽,适于分析组成复杂的多环芳烃混合物,高分子液晶是一类兼具有液晶优点和硅油优点的固定液。     

STUDIES ON SPECIFIC SEROLOGICAL ANTIGENS IN METACERCARIAE AND JUVENILES OF Paragoniums westermani AND ITS MONOCLONAL ANTIBODIES

The polyclonal antibodies to juveniles of Paragoniums westermani (PwJ-PcAbs) from sera of Wistar rats infected with Paragoniums westerrnani (P. w.)were purified by Sephadex G 200 chromatography. Next the shared serological antigens of P. w. metacercaria and juveniles (PwMJ-SAg) from the crude antigens of the metacercariae (M-NS-Ag) were purified with immuno-affinity chromatography on cyanogen bromide-activated cross-linked Sepharose 4B beads coupled withPwJ-PcAbs. PwMJ-SAg, agroup of glycoprotein molecules shown by the staining test, were specific serological antigens of P. w. metacercariae and juveniles, identified by the immunoabsorb test and immunoelectrophoresis. By SDSPAGE, PwMJ-SAg were fractionated to seven bands, including major bands A(27.SK) and Bi(19.5 K), the two major serological antigen molecules. 20 sera samples from the patients with the nonpulmonary type of P. w. paragonimiasis were detected using PwMJ-SAg and M-NS-Ag by Dot-ELISA, and the difference of sensitivity between two antigens     

卫氏并殖吸虫囊蚴-童虫特异血清学抗原及其单克隆抗体的研究

本文应用Sephadex G200柱层析从感染卫氏并殖吸虫(Pw)囊蚴的Wistar大鼠血清中提取Pw童虫多克隆抗体(PwJ-PcAbs),继用结合有PwJ-PcAbs的溴化氰激活交联Sepharose 4B进行免疫亲和层析,提取Pw囊蚴中的囊蚴-童虫血清学抗原(PwMJ-SAg),并用免疫吸收和免疫电泳证实PwMJ-SAg为囊蚴和童虫两期特异血清学抗原,其化学性质为糖蛋白。SDS-PAGE显示,该抗原有7条带,其中A(27.5kd),B(19.5kd)两带是主要血清学抗原分子。应用Dot-ELISA以PwMJ-SAg和Pw囊蚴粗抗原检测20份卫氏并殖吸虫病患者血清抗体,结果表明两种抗原检测的敏感性具高度显著性差异(p<0.001)。将PwMJ-SAg免疫短程感染Pw囊蚴的BALB/c鼠,取鼠脾细胞与SP_2/0鼠骨髓瘤细胞融合。经3次亚克隆培养,从384孔杂交瘤细胞中筛选出8个能持续分泌抗PwMJ-SAg的单克隆抗体株,它们均为IgG_1亚类。