小鼠粒细胞白血病细胞体外细胞系L_(833)的建立及其生物学特性的研究

本文用小鼠可移植性粒细胞白血病瘤株L_(801)的骨髓细胞,经体外培养建立了粒细胞白血病细胞系(株),定名为L(833)。三年来在体外已传至280余代,细胞生长迅速稳定,经不同途径动物接种致瘤率均为100％。经细胞学、细胞化学、病理学和超微结构等检查,均证明本细胞系(株)仍为粒细胞白血病细胞。L_(833)细胞系细胞染色体分析证明仍然是亚二倍体,众数39条,Y染色体丢失和存在一个标记染色体。L_(833)-A和L_(833)-B(利用半琼脂法分选出的细胞集落定名为A和B)染色体除了上述变化外,还各自存在一个标记染色体。本系细胞对所用各类抗癌药物均有不同程度的敏感性。对辐射(γ线)也很敏感,L_(833)和L_(833)-A的D_0值分别为98.8和104.9 rad。其结果和L_(801)基本是一致的,它的建立不但对于深入研究白血病和其药物筛选等有重要意义,而且利用体外瘤株进行有关研究更直观、经济和易于进行精确定量研究。     

ESTABLISHMENT OF A MODEL OF TRANSPLANTABLE MYELOCYTIC LEUKEMIA (L_(801)) IN LACA MICE

A transplantable myelocytic leukemia model of LACA mice, designated by the name of L_(801), was established by intravenous injection of spleen cell suspension from mice with radiation-induced myclocytic leukemia into mice of the same strain.Until now, for more than three years, the L_(801) has maintained stable and rapid growth and has been reproduced for over 130 serial passages. The incidence of leukemia in inoculated animals was approximately 100% and mean survival time was 10.9±2.1 days. The L_(801) is of myelocytic type which has been determined by cytological, cytochemical, pathological and ultrastructural observations. Its karyotype was hypodiploid, characterized by modal number of 39, loss of Y chromosome and an abnormal huge marker chromosome. The cell cycle duration of the L_(801) was 16 h. C-type viral particles were observed under the electron-microscope. The L_(801) was sensitive, to varying extents, to various anti-tumor agents.We presume that the L_(801) is a useful tool in studies on me     

ESTABLISHMENT AND BIOLOGICAL PROPERTIES OF MOUSE GRANULOCYTIC LEUKEMIC CELL LINE-L_(833)

A granulocytic Leukemic cell line, called L_(833), has been established through culturing in vitro frommouse bone marrow of transplantable granulocytic leukemia. More than 280 passages have been performedin 3 years. Cell growth has been rapid and stable. Incidence of tumour formation was 100% with variousroutes of inoculation. The nature of granulocytic leukemia was confirmed by examination of cytology,cytochemistry, pathology and ultrastructural changes. Analysis proved chromosomes to be hypodiploid with model number of 39, loss of chromosomes, andpresence of a marker. Besides the chromosomal change as mentioned above, both L_(883-A) and L_(833-B)derived from colonies formed from the L_(833) cells cultuerd in semi-solid agar medium, have their ownmarker. The cell line was sensitive to various types of antitumour agents in varying degrees. It was alsosensitive to ionizing radiation. D_0 values of L_(833) and L_(833-A) cells were 98.8 and 104.9 rad, respec-tively. The results were similar to that of L_(8     

小鼠可移植性粒细胞白血病瘤株L_(801)的建立

本文用脾细胞悬液给同系成年小鼠尾静脉接种建立起来的小鼠可移植性粒细胞白血病瘤株,命名为L_(801)。三年来已传130余代,瘤株生长稳定,接种成功率接近100%,平均存活时间为10.9± 2.1d.根据细胞学、细胞化学、病理学和超微结构检查,证明本瘤株为粒细胞白血病.白血病细胞染色体检查为亚二倍体,众数为39条,y染色体丢失和存在一个大的标记染色体;细胞周期为16 h;电子显微镜观察可见C型病毒颗粒;对各类抗癌药物均有不同程度的敏感性.因此,L_(801)瘤株的建立将对辐射致癌机理、辐射与病毒致癌之间关系的研究,对抗肿瘤药物筛选和实验肿瘤治疗的研究均有重要意义。     

Studies on Mechanisms and Blockade of Carcinogenic Action of Female Sex Hormones

Tumours of mice are induced by administration of Inj. Hydroxyprogesteroni Caproatis Co. (EP) in a practical subthreshold dose of carcinogenesis or 2. 5-5 times the human contraceptive dose (simply referred to as 2. 5- to 5-fold dose) combined with whole-body 0. 5 Gy gamma-ray irradiation. Malignant transformation of Syrian golden hamster embryo (SHE) cells is also induced by 5-fold dose of EP combined with 0. 3 Gy gamma-ray irradiation in vitro, thereby indicating that synergistic carcinogenesis can be obtained by combined use of physical and chemical carcinogens.The mechanisms of synergistic carcinogenesis have been further explained by cytogenetics, damage extent of the target cell DNA and production of free radicals. The Chinese traditional medicine with antioxidat-ing effect (Sulekang Capsule, SC), food additive--butylated hydroxyanisole (BHA) and green tea can effectively inhibit the carcinogenic effect of EP or EP combined with gamma rays in mice. They all have marked ability to scavenge or remove     

EXPERIMENTAL STUDIES ON THE LATE EFFECTS OF FEMALE SEX HORMONES

Inj. Hydroxyprogesterone Co. (EP) was injected i.m. into female Wistar rats, different strains of mice of both sex and Syrian golden hamsters with doses of 5--100 times the human contraceptive dose once or twice a month for 10--32 times. In some experiments EP was combined with whole-body 3 Gy gamma ray radiation once or twice. Results show that EP has obvious carcinogenicity and can enhance the gamma ray carcinogenicity, thereby increasing the tumor incidence or the ratio of malignant to benign turnouts. Carcinogenic mechanisms of EP have also been studied.     

利用陷光结构增加硅薄膜太阳能电池的吸收效率

提出了一种含有光锥光子晶体防反射层和四棱锥光栅背反射层的a-Si薄膜太阳能电池结构,吸收层厚1μm,总厚度为1.45μm。根据光子晶体及亚波长光栅的衍射特性,利用严格耦合波方法对器件参数进行了优化。计算结果表明:当光锥结构倾角θ=72°、晶格常数T1=1 200 nm、介质底半径r=100 nm时,防反射层的透射率较高,在300~600 nm波长范围内,该薄膜太阳能电池的吸收效率比不含防反射层电池提高了11.54%;当四棱锥光栅结构周期L=1.2μm、占空比f=0.38、槽深h5=560 nm时,背反射层的反射效果较好,在600~850 nm波长范围内,电池的吸收效率提高了3.75%。所设计的薄膜电池结构在波长为300~750 nm、入射角为0°~75°范围内的吸收效率均在80%以上,平均吸收效率达92%,满足太阳电池对宽频谱、广角度的光俘获的要求。     

雌性激素致癌作用的机制和对其阻断的研究

本文从实际致癌阈剂量下的小剂量复方己酸孕酮注射液(EP),折合成人的2.5倍和5倍剂量和γ线0.5Gy照射联合诱发了小鼠肿瘤;EP5倍量和γ线0.3Gy体外照射联合诱发了SHE细胞的恶性转化,进一步说明理化致癌因子联合应用可达到协同致癌作用。从细胞遗传学、靶细胞DNA损伤程度和自由基的产生进一步阐明两者协同致癌的机制。具有抗氧化作用的中药(苏乐康)、食品添加剂——BHA和绿茶等有效地抑制了致癌作用,同时观察到它们均具有较强的捕获或清除自由基、减轻DNA损伤的作用。     

雌性激素的远后效应实验研究

本文用复方己酸孕酮注射液(EP)给雌性大鼠以及不同品系雌性和雄性小鼠肌内注射,剂量按体重折合成人的5—100倍,每月注射1次或2次,共10—32次。部分实验与钴-60γ线联合应用,全身照射3Gy,1次或2次,观察其致癌和有害的非致癌效应。实验结果表明,EP具有明显的致癌作用;与γ线联合应用明显加重γ线的致癌作用。本文对EP的致癌机制也进行了讨论。