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The atrazine-resistant psbA gene of black nightshade was transferred to the chloroplast genome of atrazine-susceptible soybean by means of ovary microinjection during the stage of zygote. The identification was carried out by using the methods of spraying the leaves directly with atrazine solution, examining the change of leaf fluorescence kinetics under a brighter light induction, molecular hybridization, etc. The experimental results show that the transgenic soybean plants do have been obtained for the first time. 相似文献
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利用分子标记连锁图谱进行水稻数量基因的区间作图 总被引:6,自引:0,他引:6
以籼稻窄叶青8号(ZYQ)和粳稻京系17(JX)所衍生的F_2为材料,建立了由54个RFLP标记组成的连锁框架图。采用以侧连标记为基础的QTL区间作图法定位了影响水稻数量性状的11个QTL,它们涉及抽穗期(Dth1)、分蘖角(Ta1),每穗颖花数(Sn1、Sn2、Sn3)和颖花密度(Sdn1—Sdn6)。研究不仅为利用分子标记进行QTL作图提供了范例,同时也证实了QTL区间作图法可以发现位于两个侧连标记之间的QTL;在同一染色体上可存在影响同一数量性状的多个QTL;同类(或相关)性状在遗传上可能由同一类QTL(或多基因系统)所控制。 相似文献
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