氨基酰化酶的固定化及其对D,L—蛋氨酸的光学拆分

本文用大孔网高分子载体对氨基酰化酶进行固定化,并系统地研究了温度、pH值、离子浓度、底物浓度、激活离子及蛋白质变性剂对固定化酶活力的影响。由于载体的物理性质和功能基较适宜,因而吸附蛋白质的容量大,使固定化酶的活力和活力回收率都高。用固定化酶柱对D,L—蛋氨酸进行连续拆分,酶柱使用周期长,稳定性高。拆分的产物用离子交换法进行分离与精制后,可制得纯度高,产率理想的L—蛋氨酸盐酸盐。     

高分子支撑液膜固定化细胞甾体△1-脱氢转化的研究

研究了高分子支撑液膜固定化细胞的制备、性质以及影响其催化氢化可的松→去氢氢化可的松生物转化的各种因素,并与经典珠状包埋法固定细胞的转化作用进行了比较。结果表明前者的△¹-脱氢速率和产物转化率均高于后者。在所使用的条件下二者未见机械强度方面明显差别。     

THE IMMOBILIZATION OF AMINOACYLASE AND THE RESOLUTION OF D,L-PHENYLALANINE

A polymer with suitable physical characters and matched functional groups was synthesized,and used as a supporter to immobilize aminoacylase. The results showed that this supporterhad high immobilizing capacity and high selectivity for aminoacylase. Immobilized amino-acylase had high specific activity. In this paper, we determine the physical and chemicalcharacters of aminoacylase for resolution of D,L--phenylalanine, including its optimum tem-perature, pH, ion concentration, activated ion, substrate concentration, thermal stability anddenaturation. The immobilized aminoacylase was used to resolve the D,L-phenylalaninecontinuously. Thc resolved products was separated and purified with ion-exchange resins. L-phenylalanine solution was concentrated by vacuum evaporation and its hydrochloride wasformed. Checking of final product with polarimeter showed high yield and purity.     

氨基酰化酶的固定化和苯丙氨酸消旋体的拆分

本文合成了一种功能高分子材料,利用其适宜的物理性质和匹配的功能基来固定氨基酰化酶,并进行苯丙氨酸消旋体的拆分,研究结果表明:这种载体对氮基酰化酶蛋白固定容量大,固定化酶比活力高,活力损失小,本文系统地研究了氮基酰化酶拆分苯丙氨酸消旋体的理化性质,包括温度、pH、离子浓度、热稳定性、激活离子、底物浓度以及蛋白变性剂等,并进行苯丙氨酸消旋体的连续拆分,对拆分产物采用离子交换法进行分离精制,真空浓缩得到L-苯丙氨酸的盐酸盐,产率高,对产品进行旋光检验,纯度高。     

液膜固定化细胞甾体转化的研究

本文研究了液膜固定化简单节杆菌进行较大有机分子甾体类的生物转化,结果表明液膜固定化细胞可成功进行氢化可的松→去氢氢化可的松的△′-脱氢反应。研究了液膜固定化细胞的制备、性质和影响甾体转化的各种因素。在某些液膜体系中其转化反应初期的比活力与自由细胞相同。细胞在液膜内可存活较长时间,提高了贮存稳定性。液膜固定化细胞体系基本不受染茵的影响,而自由细胞转化作用明显受抑制。