Investigation of Tropane Alkaloids in Genetically Transformed <Emphasis Type="Italic">Atropa belladonna</Emphasis> L. Cultures

The purpose of the present study was to determine the tropane alkaloid content of genetically transformed hairy root cultures of Atropa belladonna L. Determination of alkaloids was performed by HPLC method. Samples were extracted with chloroform – methanol - cc. ammonia 15:5:1 (v/v/v). Crude extracts were purified on Extrelut columns. HPLC separation was performed on Luna C8 reversed phase column. An isocratic mixture of acetonitrile – 30 mM phosphate buffer - methanol 12.2:79.7:8.1(v/v/v) was used as eluent. Peaks were identified by addition of standards and diode-array detection. Hyoscyamine, scopolamine and apoatropine were determined by external standard method at 210 nm. We measured the alkaloid content of genetically transformed in vitro cultures (hairy roots and reorganised plants) cultivated on Gamborg B5 basic media. The highest hyoscyamine and scopolamine content was found in hairy root clone #K₅ (0,223 m/m%) and in hairy root clone #K₄ (0,018 m/m%) respectively. Alkaloid contents were higher in the hairy roots than in the reorganised plants.     

On Weyl group equivariant maps

We prove an equivariant analogue of Chevalley's isomorphism theorem for polynomial, or maps.

     

Encapsulation and diffraction-pattern-correction methods to reduce the effect of damage in x-ray diffraction imaging of single biological molecules

Short and intense x-ray pulses may be used for atomic-resolution diffraction imaging of single biological molecules. Radiation damage and a low signal-to-noise ratio impose stringent pulse requirements. In this Letter, we describe methods for decreasing the damage and improving the signal by encapsulating the molecule in a sacrificial layer (tamper) that reduces atomic motion and by postprocessing the pulse-averaged diffraction pattern to correct for ionization damage. Simulations show that these methods greatly improve the image quality.     

Bounds on Gromov hyperbolicity constant in graphs

If X is a geodesic metric space and x ₁,x ₂,x ₃ ∈ X, a geodesic triangle T = {x ₁,x ₂,x ₃} is the union of the three geodesics [x ₁ x ₂], [x ₂ x ₃] and [x ₃ x ₁] in X. The space X is δ-hyperbolic (in the Gromov sense) if any side of T is contained in a δ-neighborhood of the union of two other sides, for every geodesic triangle T in X. If X is hyperbolic, we denote by δ(X) the sharp hyperbolicity constant of X, i.e. $\delta(X)=\inf\{\delta\ge 0: \, X \, \text{ is $\delta(X)=\inf\{\delta\ge 0: \, X \, \text{ is In this paper we relate the hyperbolicity constant of a graph with some known parameters of the graph, as its independence number, its maximum and minimum degree and its domination number. Furthermore, we compute explicitly the hyperbolicity constant of some class of product graphs.     

A family of Kähler-Einstein manifolds and metric rigidity of Grauert tubes

In this paper we explain how the so-called adapted complex structures can be used to associate to each compact real-analytic Riemannian manifold a family of complete Kähler-Einstein metrics and show that already one element of this family uniquely determines the original manifold. The underlying manifolds of these metrics are open disc bundles in the tangent bundle of the original Riemannian manifold.

     

Ultra-thin-layer agarose gel electrophoresis II. Separation of DNA fragments on composite agarose-linear polymer matrices

The effect of hydrophilic linear polymer additives (non-cross-linked polyacrylamide, hydroxyethyl cellulose and polyethylene oxide) on the migration behavior of double stranded DNA molecules, ranging from 200-1000 base pairs, were studied in ultra-thin-layer agarose gel electrophoresis. The detection sensitivity was found to be less than 0.1 ng/band using To-Pro-3 fluorophore labeling and fiber optic bundle-based scanning detection system with a 640 nm red diode laser. Among the various polymers investigated, addition of linear polyacrylamide resulted in the best separation performance (steepest Ferguson plots), while composite gels with hydroxyethylcellulose and polyethylene oxide still exhibited adequate resolving power. Using the composite matrices of 1% agarose-linear polyacrylamide (0.5-3%), 1% agarose-hydroxyethylcellulose (0.2-1%) and 1% agarose-polyethylene oxide (0.2-1%), the mechanism of the separation was found to be in the Ogston sieving regime. Activation energy curves were also plotted based on the slopes of the Arrhenius plots of the various composite matrices, and exhibited decreasing characteristics for the agarose-linear polyacrylamide composite matrix and increasing characteristics for the agarose-hydroxyethylcellulose and agarose-polyethylene oxide composite matrices.     

X-ray spectrometry for preventive conservation of cultural heritage

Analytical chemistry does play a key role in the chemical characterization of the environment and it appears that X-ray spectrometry, in its many forms, is one of the most relevant analytical techniques in preventive conservation, as it is in cultural heritage research in general. X-ray spectrometry has indeed been the method of choice for the characterization of the inorganic composition of atmospheric aerosols, for a long time.