基于生物质谱技术的磷酰化修饰策略在多肽测序中的应用

该文建立了一种利用磷酰化修饰结合电喷雾质谱(ESI-Q-TOF)测定多肽氨基酸序列的有效方法。利用Atherton-Todd反应,以二丙基亚磷酰酯(DPP)为磷酰化试剂,应用生物质谱技术,对磷酰化修饰后的5种模型肽的磷酰化反应情况进行了系统研究,考察了磷酰化肽的二级质谱特征,并与未经磷酰化反应的肽的二级质谱特征对比。结果表明,经过磷酰化修饰后,肽的二级质谱中的a1离子信号强度明显增加,可以准确鉴定其N端氨基酸;b系列离子信息完整,信号强度增强,使得多肽C ID测序的谱图简单、清晰,有利于肽的氨基酸序列的测定;赖氨酸(K,128.10 u)和谷氨酰胺(Q,128.13 u)两种氨基酸质荷比相近,由于二者磷酰化修饰后的差异性,使其得到准确区分。经过5种已知氨基酸序列的模型肽的磷酰化后结合质谱技术进行氨基酸序列测定验证,结果表明该方法简单、快速、准确,提高了利用质谱技术进行多肽测序的准确度和灵敏度,可为蛋白质组学研究提供有效的技术手段。     

FUZZY控制在脲醛胶生产中的应用

以脲醛胶生产中pH值控制为应用研究背景，以模糊控制为主要控制工具，分析了脲醛胶生产中pH值的受控特点，从理论分析，工程设计方面介绍了一种应用于脲醛胶生产的pH值模糊控制器的设计，详细推导了控制表的设计过程，实现了脲醛胶生产中对pH智能控制，提出了控制规则及控制表。给出了对象参数变化情况下的MATLAB仿真曲线，最后提供了应用VB实现的控制系统的响应曲线。     

Cosmic ray test for a T0 detector

The endcap of time-of-flight (ETOF) detector in BES (Beijing Spectrometer) III is planned to be upgraded by using multi-gap resistive plate chambers (MRPCs) and the designed time resolution of the MRPCs is around 50 ps. Thus a time-zero (T0) detector needs to be built to offer a high quality reference time for the MRPCs beam test. So a T0 detector is built using plastic scintillator tiles (BC420) to couple with four fast phototubes (PMTs, Hamamatsu H6533). The timing properties of the detector is studied by using a cosmic ray test and factors related to the time resolution, such as plastic scintillator size, readout mode and angle effects, are discussed. T0 detector timing resolutions of ～41-62 ps are achieved, which means that the T0 detector can be used in the MRPC beam test.     

非线性方程的保辛近似算法

保守体系的微分方程可用Hamilton体系的方法描述,其特点是保辛。两个辛矩阵之和不能保辛,两个辛矩阵的乘积仍是辛矩阵。最常用的小参数摄动法用的是加法,因此对辛矩阵不能保辛。从保辛的角度,要用正则变换。本文针对非线性微分方程,运用自变量坐标变换,对原系统进行变换。由此推导出变换后系统的变分原理。引入Hamilton对偶变量,通过数学变换,得到变系数非线性方程。针对该方程,本文提出了保辛摄动算法。通过数值算例,对不同步长下,保辛摄动法、多尺度摄动法、龙格库塔法和精确解的结果做了比较。数值例题表明,对于非线性方程,本文提出的保辛摄动算法有良好的精度。在步长增大的情况下,保辛摄动保持了良好的稳定性。     

Experimental validation of domain-level gradient-based and hierarchical PCE-based routing and control in heterogeneous optical networks

A domain-level gradient-based routing （DLR） algorithm for heterogeneous optical networks with syn- chronous digital hierarchy and optical transport network domains is proposed and experimentally vali- dated. This algorithm classifies domains into groups with incremental levels on the basis of domain-level partitioning, and guides paths level by level along a gradient on the basis of interdomain routing tree evolution. The proposed algorithm is implemented in the hierarchical path computation element-based control architecture for connection provisioning. Testbeds with commercial and emulated nodes are es- tablished to verify the feasibility and performance of the algorithm. Experimental and emulation results show that DLR effectively performs in terms of network blocking probability, real time characteristics, and scalability.     

置氢对TC4合金粉末物理性能和压制性能的影响

采用扫描电镜观察、粉末镶嵌试样、XRD和模压成形等方法,研究了置氢对TC4合金粉末颗粒形貌、表面状态、显微硬度、显微组织、相组成和压制性能等的影响.结果表明:置氢TC4粉末颗粒形貌为不规则状,粉末基本由亮相á相及暗相a相组成,颗粒显微组织呈片状、针状和板条状.随置氢量的增大,置氢TC4粉末显微硬度总体上趋于降低;á相逐渐减少,a相逐渐增多,置氢量达到0.32%(质量分数)时出现少量的针状á″相,置氢量为0.42%和0.46%时组织为明显的板条状(?氢化物);粉末的压缩性能逐渐变好,成形性能逐渐变差,置氢量0.46%的TC4粉末的压制性能最好.     

对倾斜尖劈柱钢筋计算方法探讨

随着工程软件应用的日益普及,我国相当数量的企业已采用工程钢筋软件来完成钢筋用量的计算。在建筑业飞速发展的今天．复杂的建筑物造型,特殊的建筑构件、直接利用工程钢筋软件已无法一次完成,常常会使得预算人员力不从心。针对这种情况,在一个复杂的工程中,我们采用一种较为合理的变通方法,把一个复杂的建筑构件利用特定条件转化成两个简单建筑构件进行钢筋计算,供大家参考。     

A position-sensitive detector with lithium glass and MaPMT

A position-sensitive detector is designed for neutron detection. It uses a single continuous screen of a self-made lithium glass scintillator, rather than discrete crystal implementations, coupling with a multi-anode PMT (MaPMT). The scintillator is fast and efficient; with a decay time of 34 ns and thermal neutron detection efficiency of around 95.8% for the 3 mm thick screen, and its light yield is around 5670 photons per neutron and 3768 photons per MeV γ rays deposition. The spatial resolution is around 1.6 mm (FWHM) with the energy resolution around 34.7% by using α (5.2 MeV) rays test.     

基于LabVIEW的直流电力测功系统

直流电力测功系统利用发动机带动航空直流发电机发电,用励磁控制器控制励磁电流来稳定输出电压,通过改变电枢电流实现加载,用高精度拉压力传感器测量发动机输出的扭力,用磁电转速传感器测量输出转速,用labVIEW软件和多功能数据采集卡采集数据并输出脉冲信号控制油门控制器,实现对转速的闭环控制。