STUDIES ON THE CHARACTERISTICS OF THE HONEY-BEE SPIROPLASMA IN CHINA

Spiroplasma has been successfully isolated from the honey bee (Apis mellifera) and cultivated in R-2 medium in China. The isolate, CH-1 is obtained through triple cloning. CH-1 shows a helical morphology and a contractile movement. It is 0.17 μm in diameter and its length varies considerably with different growth periods. CH-1 forms granular-type and/or fried-egg-shaped colonies, 75—210 μm in diameter, on R-2 medium. It grows normally at temperatures ranging 25—35℃ (the optimum being 28—32℃). The optimal pH for growth is 7.0. The best growth occurs in medium containing 0.25 mol/L of sucrose. Under the optimal condition, its growth reaches the stationary phase 48 h after subculture. CH-1 cannot grow in serum-free medium and its growth is inhibited by digitonin. CH-1 can metabolize glucose, fructose, maltose, and trehalose. It can dehydrate arginine, CH-1 does not have an ability to utilize urea, nor dehydrate gelatin, nor reduce methylence blue. The taxonomic position of CH-1 has also been discuss     

转CMV－CP基因的番茄对CMV的抗性表现及遗传分析

本实验表明转ＣＭＶ－ＣＰ（ＣｕｃｕｍｂｅｒＭｏｓａｉｃＶｉｒｕｓＣｏａｔＰｒｏｔｅｉｎ）基因的番茄对ＣＭＶ的侵染有很强的抗性。外源基因在转基因番茄及其后代中的分离比例为３∶１和１∶１．     

转CMV-CP基因的番茄对CMV的抗性表现及遗传分析

本实验表明转CMV-CP(Cucumber Mosaic Virus Coat Protein)基因的番茄对CMV的侵染有很强的抗性。外源基因在转基因番茄及其后代中的分离比例为3:1和1:1。     

蜜蜂螺原体基本性状的研究

在我国首次用R-2培养基从蜜蜂上分离到的一个螺原体分离物CH-1,具有典型的螺原体形态结构和运动性。菌体直径0.17μm,长度随不同的生长时期而变化。在固体R-2培养基上形成直径75—210μm的煎蛋形或圆形菌落。CH-1生长温度范围25—37℃,最适28—32℃;最适生长pH7.0;渗透压0.25mol/L蔗糖浓度;在最适生长条件下,48h达到生长高峰。CH-1生长需要提供血清,并受毛地黄皂苷的抑制;可利用葡萄糖、果糖、麦芽糖、海藻糖和精氨酸;不能利用尿素;不能水解明胶;对甲基蓝无还原能力。