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本文研制了一种基于IrOx薄膜的阻抗型免疫传感器用于简单、快速灵敏地检测人血清中癌胚抗原(CEA)浓度。采用在金电极表面同时电化学沉积IrOx薄膜和蛋白A(PA),构建成传感界面介导抗体的定向固定。着重考察了传感检测的最优条件:电化学沉积时间及PA的浓度。在最优化的实验条件下,测得CEA 的浓度为36.2 - 460.0 ng/mL,检测下限为28.0 ng/mL。同时,采用该分析方法检测临床样品,并与化学发光免疫分析法进行了比较,实验结果表明该阻抗型免疫分析方法可推广于临床实验室CEA的免疫检测。 相似文献
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针对三电极气体火花开关工作时产生的强电磁辐射,首先对开关的导通电流及其电磁辐射进行了理论分析,利用CST电磁仿真软件对气体火花开关开展了静电场仿真研究,分析了开关短程导通时的击穿场强;其次对三电极气体火花开关的导通电流和远场辐射场强进行了实验测量,对实验结果进行分析和总结;最后采用电磁屏蔽方法对开关的强电磁辐射进行了有效抑制。研究结果可以为有关脉冲功率装置的电磁辐射及防护提供参考和借鉴。 相似文献
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A simple, rapid and sensitive impedance immunosensor based on iridium oxide (IrOx) thin film for the detection of carcinoembyronic antigen (CEA) in human sera has been proposed. Gold electrode was electrochemically modified with IrOx thin film and simultaneously functionalized with protein A (PA) to bind anti-CEA antibodies in an orientated way. It has been found that the antibody loading amount was dependent on the PA concentration and the deposition time of IrOx matrix. Under the optimized experimental conditions, the electron transfer resistances obtained were linearly related to the CEA concentration ranging from 36.2 to 460.0 ng/mL, with a detection limit of 28.0 ng/mL. Analytical results of clinical samples from cancer patients show that the proposed immunoassay is reasonably comparable with the chemiluminescence immunoassay (CLIA), indicating the feasibility of using the proposed method for CEA immunoassay in clinical laboratory. 相似文献
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