对一个多服务器环境下三因子认证协议的分析与改进

对Lwamo等提出的一个多服务环境下具有匿名性的认证协议进行了安全性分析,发现该协议无法抵抗拒绝服务攻击、智能卡被盗攻击和用户伪造攻击等.并且,针对这些安全漏洞分别给出相应改进措施.     

CRISPR-Cas12a信号放大策略与便携式血糖仪耦合定量检测黄曲霉毒素B1

建立了CRISPR-Cas12a与便携式血糖仪耦合定量检测黄曲霉毒素B1(AFB1)的方法。体系中AFB1能够激活Cas12a的反式切割活性,激活后的Cas12a切割电极上蔗糖酶修饰的发夹探针,使得蔗糖酶游离到电极表面的溶液中。蔗糖酶催化蔗糖产生可以被血糖仪监测的响应信号,进而实现对AFB1的检测。在浓度0.001～0.1 ng/mL范围内,AFB1浓度与血糖信号呈良好的线性关系,线性方程为S=3.5+229.1c,检出限为0.3 pg/mL。该方法特异性强,适用于实际样品中AFB1的检测。     

南方电网助推节能减排又有“新动作”

＂十二五＂科学发展加快转变经济发展模式,南方电网综合能源有限公司（以下简称＂南网能源＂）作为南方电网服务经济社会科学发展的绿色平台,为社会集约化、综合型的能源服务,以可持续性能源解决之道,共同推动节能减排,发展低碳经济。用绿色能源点亮万家灯火,用真诚服务传递南网情深,为建设资源节约型、环境友好型社会做出新的贡献。     

Portable biosensor for quantitative detection of melamine based on CRISPRCas12a and a personal glucose meterEI北大核心CSCD

A CRISPR-Cas system holds great promise as a next-generation biosensing technology for molecular diagnostics. In this paper, a portable biosensor based on the trans-cleavage activity of CRISPR-Cas12a and a personal glucose meter has been developed for quantitative, sensitive and specific detection of melamine. The presence of the target melamine binds to the aptamer, leading to the release of locker DNA. And then, the leasing locker DNA activates the trans-cleavage activity of CRISPR-Cas12a to cleave the single-strand DNA (ssDNA) linker on sucrase-ssDNA modified electrode, releasing a short DNA fragment labeled with sucrase in the resulting solution. The sucrase could further catalyze sucrose to glucose, which could be detected by the PGM. Under the optimized conditions, the increase of PGM signal was relative with the concentration of melamine ranging from 0.1 to 2.5 μmol/L and the limit of detection (LOD) was 37 nmol/L. Moreover, the portable biosensor has strong specificity and can be used for the quantitative detection of melamine in milk samples. © 2023, Youke Publishing Co.,Ltd. All rights reserved.