CLONING, EXPRESSION AND CHARACTERIZATION OF Pro3 GENE OF YEAST Saccharomyces cerevisiae

The proline biosynthetic pathway and Pro genes in Saccharomyces cerevisiae have just begun to be studied recently. In our laboratory, Pro2 gene of S. cerevisiae had been cloned in yeast. As described in this paper, yeast Pro3 gene was also cloned, which can complement yeast Pro3 mutants, and be expressed efficiently in E. coli. The high activities of this gene product, L-pyrroline-5-carboxylate (P5C) reductase, can be detected in both organisms. The activity of the Pro3 gene product in multiple copy plasmids is not higher than that of single copy genes in chromosomes in both yeast and E. coll. The preliminary characterization of the gene is also reported.     

高分子药物研究——Ⅲ.主链含5-氟尿嘧啶聚酯的合成及其药效的初步测定

本文报道四种新的主链含5-氟尿嘧啶聚酯型高分子抗肿瘤药物的合成及其中一种聚酯PFuE-Ⅱ-4药效的初步测定。结果表明,它具有较好的抗肿瘤活性,毒副作用小和缓释长效的特点。     

荷电颗粒在旋转环形通道内分离性能研究

为研究带电旋转环形通道内荷电颗粒的运动和沉积特性,本文使用计算流体力学两相流离散颗粒法对带电旋转环形通道内的荷电颗粒的运动过程进行了模拟。根据模拟结果分析了不同粒径、电压、入口雷诺数和通道长径比等参数对荷电颗粒运动和沉积的影响,研究了荷电颗粒在旋转通道内离心力与电场力之间的竞争关系,探索了离心力和电场力导致的荷电颗粒运动和沉积变化的规律。结果表明,单个不同粒径颗粒具有不同的颗粒逃逸电压区间,区间的大小随着颗粒粒径的增大而增大,且区间的宽度随着通道长径比的增大将会明显变小;多个颗粒的逃逸率曲线,不同粒径的颗粒将会有不同程度的交叉,随着长径比的增大,颗粒逃逸率曲线的高度与交叉会有明显的减小,而随着转速的增大,颗粒逃逸率曲线的交叉会有一定程度的减小,且高度不会有明显变化。     

Neutron powder diffraction and high-pressure synchrotron x-ray diffraction study of tantalum nitrides

Tantalum nitride(TaN) compact with a Vickers hardness of 26 GPa is prepared by a high-pressure and hightemperature(HPHT) method. The crystal structure and atom occupations of WC-type TaN have been investigated by neutron powder diffraction, and the compressibility of WC-type TaN has been investigated by using in-situ high-pressure synchrotron x-ray diffraction. The third-order Birch–Murnaghan equation of state fitted to the x-ray diffraction pressure–volume(P–V) sets of data, collected up to 41 GPa, yields ambient pressure isothermal bulk moduli of B'_0= 369(2) GPa with pressure derivatives of B 0= 4 for the WC-type TaN. The bulk modulus of WC-type TaN is not in good agreement with the previous result(B_0= 351 GPa), which is close to the recent theoretical calculation result(B_0= 378 GPa). An analysis of the experiment results shows that crystal structure of WC-type TaN can be viewed as alternate stacking of Ta and N layers along the c direction, and the covalent Ta–N bonds between Ta and N layers along the c axis in the crystal structure play an important role in the incompressibility and hardness of WC-type TaN.     

使用小型中子源的活化分析

(一)绪言 F.約里奥(Joliot)和1.居里(Curie)发現人工放射性同位素不久:赫弗西(lievesy)等用鐳鈹中子源照射含镝的钇,与已知量的样品比較,成功地測出镝的含量为0.1％。之后,由意大利罗耳拉(Rolla)教授的委託,他們又用100毫克的Ra-Be中子源六个,以石蜡为减速剂,測定了氧化钇中0.1％的铕。活化分析就是这样由赫弗西开始的。以后受中子流密度的限制,没有很大发展。自从带电粒子的加速器以及原子反应堆的出現和利用,才有了今日的盛况。近来,由于测定仪器的进展及各有特色的小型中子源也较易得到,如用手提式的中子源作非破坏的分     

驱动马达与轨道之间纳米摩擦的随机动力学研究

研究了驱动马达与轨道之间纳米摩擦的微观机制.通过基于克拉默斯(Kramers)理论的"键断裂"模型,得到了驱动马达与轨道之间纳米摩擦与马达行走速度之间关系.研究表明我们的理论结果与实验吻合.     

一种基于比例因子的慢启动策略

TCP慢启动机制中拥塞窗口大小指数增长,会导致在慢启动前期发送速率偏低的现象。针对此问题,本文提出一种基于比例因子的慢启动策略,该策略实现了慢启动到拥塞避免的快速过渡,并保证了数据传输的总体效率。实验表明,新策略能明显提高网络吞吐量,利于网络资源利用。     

基于岸站二维投影采集的波浪观测补偿算法

针对海洋环境监测中视频采集设备因拍摄条件限制引起的梯形失真、倾斜失真和波高失真三类主要问题，提出一种以二维投影的三维还原为基础的视频采集处理的补偿算法。通过坐标和倾角偏移量补偿，还原失真的目标图像，并在此基础上进行目标测量信息处理，从而得到更加准确合理的测量结果。实验证明，该算法可根据任意位置参数实现失真图像的准确修复，精度高且易于实现。     

荧光光谱法研究盐酸氟奋乃静与DNA相互作用

采用荧光光谱法研究了FPZ与DNA的相互作用机理.实验结果表明,DNA通过静态猝灭机制使FPZ的荧光发生猝灭,疏水作用力和氢键在FPZ-DNA复合物形成过程中发挥主要作用,FPZ和DNA的相互作用模式是嵌插结合方式.