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红假单胞菌的分离鉴定与生长特性 总被引:4,自引:0,他引:4
由青岛沿岸潮间带表层沉积物分离出4株光合细菌,经鉴定属于荚膜红假单胞菌和球形红假单胞菌。光照厌氧培养生长实验表明HD3的最适盐度为S=15-25,PS2-2为一淡水菌株。菌株HD3的最适PH范围为6.35-8.3。HD3、RS均不能利用硫化物且其生长受较高浓度的硫化物抑制。 相似文献
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本文证明鸡心线粒体辅酶Q-细胞色素c还原酶,于低离子强度下经Triton X-100增溶,亲和层析分离,得到单分散相的酶-Triton复合物。每微克分子酶含有低于0.15μmol辅酶Q以及12μmol的磷脂。还原态鸡酶的细胞色素b在α区表现两个吸收高峰,位于558,562nm;细胞色素c_1的α-吸收峰为553nm。SDS凝胶电泳指出,纯化的酶复合物至少由9个亚基组成。在高浓度盐和Triton X-100的作用下,酶复合物被解离为亚基复合物。三只氧化还原亚基(细胞色素b,c_1和Fe-S蛋白)被进一步分离、纯化。 相似文献
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在0.02mol/dm~3NH_4Cl-0.02mol/dm~3TEA-1.5×10~(-4)mol/dm~3BTZ底液中,悬汞电极上,Cu(Ⅱ)与苯并三氮唑配合物有一灵敏的极谱吸附不可逆还原波。本文用半微分极谱法进行了研究。峰电位随扫描速率增加而负移。当扫速为100mV/s时,Ep为-0.65V(vs.SCE)。t_(ads).60s时,峰高与铜浓度0.0005~0.065μg/ml之间存在正比关系。测得吸附配合物组成Cu:BTZ为1:1,条件形成常数β=3.5×10~4。在电极表面上,配合物的吸附近似服从Langmuir吸附等温式,其吸附系数α=7.9×10~4。方法应用于人体血清、头发、胃液和胃癌组织中微量铜的分析,与阳极溶出伏安法相对照,结果满意。 相似文献
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随着芯片技术、软件技术和计算机通信技术等的革命性突破,下一代网络(NGN)将逐步替代PSTN。简要介绍了NGN的网络模型、典型特征及NGN业务的特点,预测了NGN业务的发展前景。 相似文献
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Chicken heart mitochondrial ubiquinol: cytochrome c reduetase has been isolated as a monodisperse form by solubilization with Triton X-100 in low ionic strength and subsequent affinity chromatography. The enzyme complex contains less than 0.15μmol ubiquinone and 12 μmol phospholipid per μmol enzyme. The spectrum of cytochreme b in the reduced enzyme exhibited two maximum α-absorption bands at 558nm and 562nm; a shoulder at 553 nm for c_1. The purified enzyme complex consists of at least 9 subunits with apparent molecular weight of 47K, 44K, 36K, 31K, 25K, 17K, 15K, 12K and 9K. The enzyme complex has been dissociated into subdomains of subunits in the presence of increasing concentration of salt and Triton X-100. Three subunits carrying the redox centers (cytochrome b, cytochrome c_1, and Fe-S protein) have been further isolated. 相似文献
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海湾扇贝(Argopecten irradians)附着基异养细菌区系初探 总被引:1,自引:0,他引:1
从海湾扇贝附着基上分离出61株异养细菌,对其进行80项生理生化特征测定,以数值分类法进行分析。鉴定结果表明,海湾扇贝附着基上异养菌属于假单胞菌(Pseudomonas)、气单胞菌(Aeromonas)、弧菌(Vibrio)、芽孢杆菌(Bacillus)和产碱杆菌(Alcaligenes)。扇贝幼虫附着前附着的细菌主要为芽孢杆菌与假单胞菌,扇贝幼虫附着后气单胞菌成为优势,弧菌数量明显增加,与假单胞菌数量相当。 相似文献
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The mechanism of shocklets is studied theoretically and numerically for the stationary fluid, uniform compressible flow, and boundary layer flow. The conditions that trigger shock waves for sound wave, weak discontinuity, and Tollmien-Schlichting (T-S) wave in compressible flows are investigated. The relations between the three types of waves and shocklets are further analyzed and discussed. Different stages of the shocklet formation process are simulated. The results show that the three waves in compressible flows will transfer to shocklets only when the initial disturbance amplitudes are greater than the certain threshold values. In compressible boundary layers, the shocklets evolved from T-S wave exist only in a finite region near the surface instead of the whole wavefront. 相似文献
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