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本研究通过提取不同培养条件下的粗毛栓菌总RNA,利用RT-PCR的方法克隆到1个新的漆酶基因,命名为Tglac3,编码区长1,560bp,预测编码519个氨基酸残基,其分子量为56.6kDa,理论等电点为5.77.用荧光定量RT-PCR法分析了该漆酶基因在不同培养条件下的表达水平,结果显示,高浓度的碳源和氮源均能诱导Tglac3基因的表达. 相似文献
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The visualization techniques were explored for power quality monitoring.And remote visualization solutions were proposed for highspeed rail power quality monitoring.Taking the Beijing-Shanghai highspeed rail power monitoring as a study case,a remote visualization client,based on our proposed solutions,was developed for high-speed rail power quality monitoring to efficiently support power quality data analysis of the electricity business.The solutions collected data from monitoring stations deployed along the high-speed rail route and visualized the data set with a variety of visualization technologies to alert the specific stations of catastrophic events.The proposed solutions have been proved to be effective in supporting decision-making for the railway power scheduling and providing diagnosis information for quickly spotting any possible runtime failure in operation. 相似文献
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化症液治疗病窦综合征疗效观察 总被引:1,自引:0,他引:1
观察自制中药制剂化主射液治疗病态窦房结综合征的疗效,探索化症液应用新指征,方法:对36例病窦综合征患者用化症液治疗,进行前瞻性研究,采用心电图、动态心电图、食管电生理检查评价窦房结功能,结合临床评判进行疗效评判,并随访0.5~3.5年。结果:化症液治疗1~4个疗程后全部病例均有明显疗铲,临床症状、心电图、动态心电图及窦房结功能均有不同程序改善。晕厥症状消失,治疗后平均提高心率达12次/min;治疗 相似文献
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为了提高外源基因整合到染色体的效率,在已构建的运动发酵单胞菌-大肠杆菌穿梭载体基础上,构建了RecET表达质粒pSUZM3a-RecET.选取乙醇脱氢酶Ⅰ(adhA)基因作为靶基因,四环素抗性基因(Tcr)作为筛选标记基因,检测RecET重组系统在Z.mobilis中进行基因重组的可行性.将两端带有60bp adhA基因同源臂的四环素抗性基因片段电击转化含有RecET重组系统表达质粒的运动发酵单胞菌ZM4,获得adhA基因缺失突变菌株.对突变菌株adhA基因的PCR产物进行测序发现,adhA基因已被置换为四环素抗性基因.上述结果表明:RecET重组系统在运动发酵单胞菌中具有高效、便捷和可操作性,只需60bp同源臂即可完成同源重组. 相似文献
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