自制甲基硝基亚硝基胍诱发大鼠胃腺癌及其幽门腺区上皮细胞的改变

使非纯系大白鼠饮自制甲基硝基亚硝基胍溶液,并给每鼠每周加灌饱和食盐溶液1毫升,18个月期间,在实验组18只大白鼠中出现胃腺癌5例,12指肠腺癌2例,此外尚有少数前胃乳头状瘤及肺癌.在对照组17例中则未见肿瘤.显微镜下,可见实验组幽门腺区胃小凹深部上皮细胞有显著增生,癌变亦起自该处.电镜下可见幽门腺区胃小凹上皮细胞增生者仅有核浆比例略增大,有不典型增生者的核形状、基底膜和细胞间联接等的改变接近癌细胞.文中认为增大致癌剂浓度及加长接触时间,可在不敏感动物中诱发出癌变.     

ESTABLISHMENT AND CHARACTERIZATION OF A CELL LINE HR-8348 DERIVED FROM HUMAN POORLY DIFFERENTIATED ADENOCARCINOMA OF RECTUM

A human rectal poorly-differentiated adenocarcinoma cell line HR-8348 is established from thesurgically removed tumor mass of a male adult patient. The cell doubling time is 36.05 h. The mitoticindex is 44.5‰. The cells are all of epithelial type and mostly of polygonal shape. Electron microscopicexamination shows malignant nuclei with enlarged nucleoli; there are abundant microvilli and some se-cretory granules. The plating efficiency in soft agar is 12.9%. In PHA (1μg/ml), the cells show obviousagglutination. Chromosomal analysis reveals a mode of 70 chromosomes per cell, with a marker chromo-some. CEA in cells is detected positively both qualitatively and quantitatively. Hetero-transplantation ofthe cells to nude mice shows that the tumor mass has the same morphology as the original mass fromthe patient, with Alcian blue positive material in the cytoplasm. Repeated detection for mycoplasma con-tamination shows negative.     

~(131)I标记抗人结肠癌单克隆抗体MC_3对裸鼠载人肠癌的放射免疫显像和导向治疗研究

用~(131)I标记的抗人结肠癌单克隆抗体(McAbMC_3)对裸鼠载人肠癌进行放射免疫显像诊断和实验性治疗研究。结果显示:体外标记抗体特异性结合率分别为37.5％和32.1％。裸鼠体内在48~120h的ECT照相可见在肿瘤部位均有放射性的特异性浓聚,其摄取量随时间延长逐渐增加,肿瘤显影清晰,显像的合适时间为96～120h。而给予非特异性的~(131)I-NMIgG后肿瘤部位来见放射性浓聚,而呈全身均匀性分布。120h肿瘤组织与肝脏及正常肠组织的比值分别为3.61和9.81,肿瘤定位指数为4.26。实验治疗显示与对照相比~(131)I-MC_3对肿瘤有明显的抑制生长作用,治疗后第14天肿瘤抑制率为90.14％,与~(131)I-NMIgG对照组比较差异极显著(P<0.01)。治疗后第32天裸鼠血清CEA含量与~(131)I-NMIgG组比较差异显著(P<0.05)。病理组织学检查结果显示治疗后8天注射~(131)I-MC_3裸鼠肿瘤呈大片坏死,仅局部肿瘤边缘尚存少数完整的肿瘤细胞,而其它正常组织、器官未见明显辐射损伤。提示McAbMC_3用于肠癌的诊断和导向治疗可能有良好的前景。     

人直肠腺癌细胞系——HR-8348的建立和生物学特性

从一例男性直肠腺癌患者手术后,取得原发癌肿组织,经离体培养建成细胞系,定名为HR-8348。对本系细胞进行生物学特性的观察分析结果是:细胞在对数增殖期的倍增时间为36.05h,细胞分裂指数在第三天达高峰,为44.5‰。细胞形态以多边形上皮样细胞为主,在电子显微镜下可见细胞核及核仁呈恶性细胞特征,细胞表面有微绒毛.细胞核形态不规则,胞浆中核蛋白体较丰富,可见分泌颗粒,在少数细胞可找见张力原纤维柬以及细胞间的桥粒结构。AB染色反应阳性,细胞在半固体琼脂中集落形成率为12.9％。细胞在1μg/ml的PHA中即有凝集反应。细胞的染色体众数范围为69—75,有长的亚近端着丝点标记染色体。用定性和定量分析均证实该细胞具有分泌CEA的能力。小鼠皮下移植形成恶性肿瘤结节,其组织结构与该病例的临床直肠癌标本相似,该细胞系经多次检查未发现支原体污染。