君子兰花粉散粉前后合成的核糖核酸和蛋白质的作用

本文证明君子兰花粉在RNA和蛋白质合成完全被抑制的条件下仍可达到95％右左的萌发率,并长出有限长度的花粉管。RNA酶和蛋白酶均可影响直至完全阻止花粉的萌发和/或花粉管的伸长。统计表明:成熟花粉内已有的蛋白质可维持花粉管约70μm的伸长,预存mRNA转译的蛋白质可维持花粉管约530μm的伸长。散粉后合成的RNA和蛋白质对花粉管后继伸长的速度和晚期生长(>2400μm)都是必需的。环己亚胺3h或放线菌素D,10h以上的处理会引起花粉管畸形,畸变率与抑制剂浓度及处理时间相关。适量的抑制剂在培养的头5h内可阻碍生殖细胞通过分裂中期,10h以后加抑制剂,不再影响精细胞的形成。     

莲子叶蛋白体的类型,来源与发育

莲子叶于发育的１０￣２８ｄ,以不同速率在叶肉细胞中大量积累贮存蛋白,并以不同方式组装许多形态各异、结构不同的蛋白体。按其来源与发育的特点可分４种类型：①粗面球状蛋白体;②光面球状蛋白体;③杯状蛋白体;④淀粉蛋白体。发育顺序是①,②类先形成,③,④类后发生;②类的发育贯穿整个贮存蛋白的积累期,比较了莲与其他植物蛋白体的异同。     

冷却型科学级CCD荧光影像系统对细胞多参数的研究

应用冷却型科学级ＣＣＤ摄像式显微荧光影像系统对受精后２０ｄ（Ｓ２０）的红莲子叶细胞进行了ＤＮＡ,ＲＮＡ和总蛋白相对质量含量的多参数相关测量。     

THE ROLE OF RNA AND PROTEIN SYNTHESIZED BEFORE AND AFTER DEHISCENCE OF THE ANTHER IN THE DEVELOPMENT OF POLLEN GRAINS OF Clivia nobilis

Under the condition of completely inhibited RNA and protein synthesis, the rate of pollen grain germination of Clivia nobilis still reaches about 95% and the pollen tubes grow up to a certain length.RNase or pronase E added to the medium affected the germination and the elongation of the pollen tubes till they were completely blocked. Statistical results show that the original proteins may maintain the elongation of pollen tubes at about 70 μm, the proteins translated from pre-existing mRNA maintain them at about 530 μm.The RNA and protein synthesized after dehiscence of the anther are necessary for the normal rate of subsequent elongation and the growth of pollen tubes in the later stage (>2400 μm). Treatment of long duration such as CHM 3h and AmD 10h gives rise to aberration of pollen tubes, the rate of aberration being related to the concentration of inhibitors and the duration of treatment.In the first 5h of culture a suitable amount of inhibitors may block the progress of meta- phase of the gen     

莲子贮存蛋白基因的表达与子叶叶肉细胞的变构

莲子贮存蛋白基因（ＳＰＧ）的表达受发育调控。子叶叶肉细胞变构早于ＳＰＧ的开始表达,随发育过程加剧。在贮存组织中细胞变构的发生有严格的时空顺序性。变构主要表现在细胞核体积增大,外形变不规则,染色质分布不均匀化,核膜局部消失。     

莲子蛋白的组成,溶性和品质

广昌干白莲莲子中含蛋白含量分数为２４．９４％,比其他３０个品种的平均值高１５％,其氨基酸组成中必需氨基酸的比例符合卫生组织规定要求,盐溶球蛋白和水溶清蛋白占总蛋白含量的７６．２３％,醇溶蛋白仅占０．３３％。主要蛋白亚基有８个,相对分子质量在９８×１０＾３￣１６×１０＾３之间,讨论了莲子贮存蛋白基因作为优秀蛋白基因材料的应用前景,还就其种子蛋白的贮存部位,含量,溶性及必需氨基酸的比例论证了莲归属双子