排序方式: 共有19条查询结果,搜索用时 265 毫秒
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座壳孢属(Aschersonia)是粉虱和介壳虫的一类重要病原真菌,可用于世界性害虫烟粉虱(Bemisiatabaci)的生物防治.本文对来自世界各地的15株座壳孢菌进行了产孢、萌发性状比较,在此基础上筛选出11株产孢、萌发能力较好的菌株测定其对烟粉虱的致病力.菌株Aa5、Ag1、Aa8、Aa7、Aa2和Aa1均表现出高致病性(>60%),此外本研究还发现菌株的致病力与其萌发率成明显正相关(r=0.86),而与产孢量不相关. 相似文献
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以非维管植物地衣为分离材料,采用热处理加抗生素及醋酸钠的方法,经常规形态学鉴定,分离到6株苏云金芽孢杆菌.为了解其生物学特性,分别对这6株菌株进行生理生化测定和cry基因的PCR-RFLP检测,结果显示: 6个菌株均含有cry1、cry1I 、cry2基因,所有菌株均不含cry3、cry4、cry5、cry6、cry8、cry10、cry11基因.初步确定6株菌株中含有5种 cry1亚类基因(cry1Ad,cry1Ba,cry1Cb,cry1Da,cry1Ea),1种cry2亚类基因cry2Ac,2种cry1I基因亚类(cry1Ia1、cry1Ib1),其中有2个菌株的PCR-RFLP图谱均出现特异性片段,推测可能存在新基因. 相似文献
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从地衣分离获得了Bt菌株BRC-ZQL3,为提高其产孢水平,应用数学统计方法对该菌株发酵培养基进行优化.通过单因子分析,研究碳源、氮源、初始pH等因子对产孢的影响;再采用2水平PlackettBurman设计,筛选影响产孢的8个因子,实验结果表明培养基成分中3个重要影响的因子,分别为CaCO3、玉米粉、酵母膏;利用响应... 相似文献
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本文介绍了基因组学与蛋白质组学和生物信息学的基本内容,列举了主要核酸、蛋白质数据库及其查询软件并说明如何利用查询工具对生物信息资源进行查询和对核酸蛋白质进行分析. 相似文献
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Ochrobactrum anthropi CTS-325 isolated from a chromium-contaminated site had better resistance to Cr(Ⅵ) in LB medium under aerobic condition.Meanwhile,it was found that the reduction of Cr(Ⅵ) is not complete during the experimental process.Therefore,a series of small molecule energy sources including nitrogen and carbon sources were added into the LB medium in the bacterial stationary phase to promote the chromium reducibility.The result showed that the bacterial growth was positively correlated with the chromium reduction.SDS-PAGE analysis indicated that the protein groups were changed when the bacteria were stimulated by the chromium.Additionally,it was revealed that O.anthropi CTS-325 could utilize the cheaper alternative of sugar(sucrose residue leaching solution) well for further growth and restart the chromium reduction,which offered a new method for practical appli-cations. 相似文献
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采用醋酸钠.抗生素分离法对超市牛奶样品进行苏云金芽胞杆菌(Bacillus thurgngiensis,Bt)的分离,获得2株Bt菌株,分别命名为BtBRC—LJ1和BtBRC-LJ2。利用PCR方法对Bt BRC-LJ1和BtB RC-LJ2菌株幼皿和nheC肠毒素基因的分布进行检测。结果显示,这2个菌株均含有这两种肠毒素基因。 相似文献
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