An Electrochemical DNA Biosensor for the Detection of the Apa I Polymorphism in the Vitamin D Receptor Gene Using Meldola's Blue as a Hybridization Indicator

Electrochemical detection of nucleic acid base mismatches related to Apa I single nucleotide polymorphism (SNP) in the vitamin D receptor gene was performed successfully using 7‐dimethyl‐amino‐1,2‐benzophenoxazinium salt (Meldola's blue, MDB) with 10.9 pmol/100 μL of detection limit. MDB reduction signals obtained from probe, mismatch(probe‐SNP containing target) and hybrid(probe‐target) modified pencil graphite electrode(PGE) increased respectively. The sensor was able to clearly distinguish perfect match from mismatch DNA in a 30 min. detection time. Several factors affecting on the hybridization and indicator response are studied to maximize sensitivity and selectivity. The advantages of the biosensor are discussed in comparison with previous electrochemical assays for DNA hybridization.     

Green’s function,temperature in a convectively cooled sphere with arbitrarily located spherical heat sources

Steady state heat conduction in a convectively cooled sphere having arbitrarily located spherical heat sources inside is treated with the method of Green’s function accompanied by a coordinate transform. Green’s function of the heat diffusion operator for a finite sphere with Robin boundary condition is obtained by spherical harmonics expansion. Verification of the analytical solution is exemplified in some generic cases related to the pebbles of South-African PBMR as of year 2000 with 268 MW thermal power. Analytical results for different sectors of the sphere (pebble) are compared with the results of computational fluid dynamics code FLUENT^™. This work is motivated through a modest effort to assess the stochastic effects of distribution and volumetric effects of fuel kernels within the pebbles of future-promising pebble bed reactors.     

Maxim GMSL SerDes器件的预加重和均衡

发送端的预加重和接收端的均衡能够使串行器/解串器(SerDes)采用廉价的电缆传输数据或延长数据传输距离。本文介绍了电缆对信号传输的影响,以及如何补偿。此外,文章介绍了使用有损电缆时,如何利用Maxim的多媒体串行链路(GMSL)产品构建可靠链路。本文还简要介绍了线路均衡技术。     

Microwave-assisted dilute acid pretreatment of different agricultural bioresources for fermentable sugar production

Microwave-assisted pretreatment can be used for fermentable sugar production from lignocellulosic biomass. In this study, the optimum hydrolysis conditions of barley husk, oat husk, wheat bran, and rye bran were determined in power level, treatment time, solid-to-liquid ratio and dilute acid ratio as follows: 700 W, 6.92 min, 1:18.26 w/v, and 3.67% for barley husk, 600 W, 6.96 min, 1:17.22 w/v, and 3.47% for oat husk, 600 W, 6.92 min, 1:16.69 w/v, and 1.85% for wheat bran, and 460 W, 6.15 min, 1:17.14 w/v, and 2.72% for rye bran. The fermentable sugar concentrations were 37.21 (0.68 g/g), 38.84 (0.67 g/g), 49.65 (0.83 g/g), and 36.27 g/L (0.62 g/g) under optimum conditions, respectively. The results showed that microwave-assisted pretreatment is a promising technology which can be successfully implemented for the hydrolysis of lignocellulosic biomass for high sugar yield. On the other hand, hydrolysates included some inhibitors such as organic acids, furans, and phenolic compounds. Lignocellulosic biomass used in this study can be employed as good feedstocks for value-added product production in the fermentation process, after the inhibitors have been detoxified/removed with different detoxification methods.     

Synthesis and Characterization of Crown-Ether-Containing Phthalocyanines with Group-IV-A Elements

Group-IV-A phthalocyanines with four crown ether substituents have been prepared from 4′,5′-dicyanobenzo(15-crown-5), 4′,5′-diiminoisoindolino(15-crown-5), or metal-free phthalocyanine and the corresponding metal salts. The axial ligands of dichloro[tetra(15-crown-5)phthalocyaninato]silicon or -tin have been converted into dihydroxy derivatives by hydrolysis in aqueous Et₃N. The catalytic effect of H₂O-free CaC1₂ in quinoline is used for the polycondensation of dihydroxysilicon-phthalocyanine to cofacially arrayed polymers. The thermal stability of group-IV-A-metal phthalocyanines is confirmed by the higher initial decomposition points (290–440°) compared to those of the corresponding transition-metal phthalocyanines.     

Two-body Dirac equation versus KDP equation

A brief review of two-body Dirac and Kemmer-Duffin-Petiau approaches for the bound state problem of two fermions is presented from an algebraic point of view in a comparative manner. Reduction of the direct product of two Dirac spaces is discussed.Work supported by the T.B.T.A.K. under TBAG/CG-1.     

Utilization of a unitary transform for efficient computation in the matrix pencil method to find the direction of arrival

In this study, we use the matrix pencil (MP) method to compute the direction of arrival (DOA) of the signals using a very efficient computational procedure in which the complexity of the computation can be reduced significantly by using a unitary matrix transformation. This method applies the technique directly to the data without forming a covariance matrix. Simulation results show that the variance of the estimate approaches to the Cramer-Rao lower bound. Using real computations through the unitary transformation for the MP method leads to a very efficient computational methodology for real time implementation on a digital signal processor chip. A unitary transform can convert the complex matrix to a real matrix along with their eigenvectors and thereby reducing the computational cost at least by a factor of four without sacrificing accuracy. This reduction in the number of computations is achieved by using a transformation, which maps centro-hermitian matrices to real matrices. This transformation is based on Lee's work on centro-hermitian matrices.