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Nghia Huynh Gabriele Beltrame Marko Tarvainen Jukka-Pekka Suomela Baoru Yang 《Molecules (Basel, Switzerland)》2022,27(6)
Triterpenoids are among the bioactive components of Chaga, the sterile conk of the medicinal fungus Inonotus obliquus. Supercritical fluid extraction of Chaga triterpenoids was carried out with supercritical CO2, while a modified Folch method was used as a comparison. Three temperature-pressure combinations were tested varying between 314–324 K (40–50 °C) and 281–350 bars, using time- and volume-limited extractions. Six triterpenoids were identified with GC-MS and quantified with GC-FID: ergosterol, lanosterol, β-sitosterol, stigmastanol, betulin, and inotodiol. The Folch extraction resulted in recovery of trametenolic acid, which was not extracted by supercritical CO2. Inotodiol was the major triterpenoid of all the extracts, with a yield of 87–101 mg/100 g and 139 mg/100 g, for SFEs and the Folch method, respectively. The contents of other major triterpenoids, lanosterol and ergosterol, varied in the ranges 59–63 mg/100 g and 17–18 mg/100 g by SFE, respectively. With the Folch method, the yields were 81 mg/100 g and 40 mg/100 g, respectively. The highest recovery of triterpenoids with SFE in relation to Folch was 56% and it was obtained at 324 K (50 °C) and 350 bar, regardless of extraction time or volume of CO2. The recoveries of lanosterol and stigmastanol were unaffected by SFE conditions. Despite the lower yield, SFE showed several advantages including shorter extraction time and less impact on the environment. This work could be a starting point for further studies on green extraction methods of bioactive triterpenoids from Chaga. 相似文献
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Affects of microwave bandwidth on the high-charge-states of ion beams extracted from a conventional minimum-B-geometry ECR ion source are first demonstrated. The high-charge-state intensities, produced with broadband microwave radiation are observed to be factors ?2 than those produced with narrow bandwidth microwave radiation at the same power level. 相似文献
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Conservative methods for the Toda lattice equations 总被引:1,自引:0,他引:1
We are concerned with the numerical integration of the Todalattice equations by using different conservative methods. Numericalexperiments suggest that the global error for isospectral schemesdecreases exponentially with time but it is almost constantfor either symplectic or more general integrators. We providea theoretical explanation for these experimental findings. 相似文献
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Schwarz domain decomposition methods are developed for the numerical solution of singularly perturbed elliptic problems. Three
variants of a two-level Schwarz method with interface subproblems are investigated both theoretically and from the point of
view of their computer realization on a distributed memory multiprocessor computer. Numerical experiments illustrate their
parallel performance as well as their behavior with respect to the critical parameters such as the perturbation parameter,
the size of the interface subdomains and the number of parallel processors. Application of one of the methods to a model problem
with an interior layer of complex geometry is also discussed.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
9.
A shape optimization problem for incompressible flows within a stabilized finite element framework is studied. The goal is to develop and test numerical realizations of optimal shape design problems that could be applied to non‐trivial industrial problems. The resulting algorithm is applied to the optimization of the geometry of a tapered header in a paper machine headbox. Copyright © 2000 John Wiley & Sons, Ltd. 相似文献
10.
Rissanen SM Kankaanpää M Tarvainen MP Novak V Novak P Hu K Manor B Airaksinen O Karjalainen PA 《IEEE transactions on bio-medical engineering》2011,58(9):2545-2553
Deep brain stimulation (DBS) is effective in reducing motor symptoms in Parkinson's disease (PD). However, objective methods for quantifying its efficacy are lacking. We present a principal component (PC)-based tracking method for quantifying the effects of DBS in PD by using electromyography (EMG) and acceleration measurements. Ten parameters capturing PD characteristic signal features were initially extracted from isometric EMG and acceleration recordings. Using a PC approach, the original parameters were transformed into a smaller number of PCs. Finally, the effects of DBS were quantified by examining the PCs in a low-dimensional feature space. The EMG and acceleration data from 13 PD patients with DBS ON and OFF, and 13 healthy age-matched controls were used for analysis. Clinical evaluation of patients showed that their motor symptoms were effectively reduced with DBS. The analysis results showed that the signal characteristics of 12 patients were more similar to those of the healthy controls with DBS ON than with DBS OFF. These observations indicate that the PC-based tracking method can be used to objectively quantify the effects of DBS on the neuromuscular function of PD patients. Further studies are suggested to estimate the clinical sensitivity of the method to different types of PD. 相似文献