Dynamics of Bacteriorhodopsin in the Dark-Adapted State from Solution Nuclear Magnetic Resonance Spectroscopy

To achieve efficient proton pumping in the light-driven proton pump bacteriorhodopsin (bR), the protein must be tightly coupled to the retinal to rapidly convert retinal isomerization into protein structural rearrangements. Methyl group dynamics of bR embedded in lipid nanodiscs were determined in the dark-adapted state, and were found to be mostly well ordered at the cytosolic side. Methyl groups in the M145A mutant of bR, which displays only 10 % residual proton pumping activity, are less well ordered, suggesting a link between side-chain dynamics on the cytosolic side of the bR cavity and proton pumping activity. In addition, slow conformational exchange, attributed to low frequency motions of aromatic rings, was indirectly observed for residues on the extracellular side of the bR cavity. This may be related to reorganization of the water network. These observations provide a detailed picture of previously undescribed equilibrium dynamics on different time scales for ground-state bR.     

Ueber Sulfos?uren in Harn

Ohne Zusammenfassung     

Projektive Punktskalen und Summationsformeln für Tschebyscheff-Polynome

Zusammenfassung. Mit Hilfe der projektiven Geometrie werden neuartige Summationsformeln hergeleitet, die die Tschebyscheff-Polynome und modifizierte Polynome enthalten; z. B. gilt für alle : Daraus ergeben sich wiederum interessante Summationsformeln für die Hyperbelfunktionen; es gilt z. B. für positive reelle Zahlen t: Eingegangen am 28.02.96, revidierte Fassung am 25.08.97 / Angenommen am 09.10.97     

Comparative study of the cope rearrangement of hexa-1,5-diene and barbaralane

The classical rules for Cope rearrangements predict a transition state with chair form to be favored over the boat form. On the other hand, bridged homotropylidenes, which allow only a boat-form transition state by steric reasons, have extremely low barriers. A controversy about the correct pathway and the different possible intermediates and transition states of the reaction has gone on for years. In this work, the hypersurfaces of barbaralane, in comparison with the boat- and chair-form of hexa-1,5-diene, are computed by the ab inito CASSCF (6,6)/6-31G** method starting with UMP2/6-31G** natural orbitals (NO's). All three hypersurfaces show characteristic features, and, moreover differ from each other. A hitherto undiscussed intermediate, bicyclo[2.2.0]hexane, was localized on the boat-hexa-1,5-diene pathway. So it is noteworthy that our transition state for the boat-hexa-1,5-diene does not correspond to the transition states found by other authors for this conformation. The computed enthalpies of activation of boat- and chair-hexa-1,5-diene, and barbaralane are in good agreement with the experimental data.     

Modular FET model with distributed source configuration for single and double side source grounded MESFET

A new approach for accurately modelling MESFETs and HEMTs up to the high millimetre-wave range is described. The use of a distributed source configuration allows the characterisation of both single side grounded (SSG) and double side grounded (DSG) FETs in the common source and also in the reverse channel circuit configuration. The model has been verified up to 40 GHz with excellent results. To the authors' knowledge the effect of SSG has not been examined yet by any commercial CAD tool.<>     

Feasibility Study of Batteryless Temperature Transponders Using Miniature Microwave Cavity Resonators

Batteryless temperature transponders have been constructed and tested. The design employs a sensing element consisting of a miniature cavity resonator loaded with a ceramic material of high dielectric constant and large temperature coefficient of resonant frequency. This is connected by thin coaxial cable to a small dipole or sector antenna. Prototypes with sensors 3 mm in diameter have been fabricated and can resolve temperature differences of 0.1°C in air. Signals have been obtained from transponders implanted in anesthetized rats, but antenna orientation is a critical and limiting factor that will require further efforts before a system is capable of operating in freely roaming animals.     

Comprehensive Design of a Three-Phase Three-Switch Buck-Type PWM Rectifier

A three-phase three-switch buck-type pulsewidth modulation rectifier is designed for telecom applications in this paper. The rectifier features a constant 400-V output voltage and 5-kW output power at the three-phase 400-V mains. The principle of operation and the calculation of the relative on-times of the power transistors are described. Based on analytical relationships the stresses of the active and passive components are determined and the accuracy of the given calculations is verified by digital simulations. Exemplarily, a 5-kW power converter is then designed based on the analytical expressions and on switching loss measurements from a hardware prototype constructed with insulated gate bipolar transistor/diode power modules. The loss distribution of the components, the total efficiency, and the junction temperatures of the semiconductors are then evaluated in dependency on the operating point. Finally, the trade-off between the selected switching frequency and the admissible power range for the realized design is shown and a total efficiency of 95.0% is measured on the hardware prototype, where an excellent agreement with the theoretically evaluated efficiency is shown     

Luminescence kinetic and association equilibria of lanthanide ions in aqueous solutions. II. Determination of the formation constant of Tb acetato complex by means of luminescence lifetime measurement

The association constant K of mono-acetato complex of Tb³⁺ has been determined at normal p and T conditions by measurement of its luminescence lifetime in aqueous solution containing increasing concentrations of acetate. Two experimental arrangements used for the measurement are described.