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消癌平诱导人肺腺癌(ASTC-a-1)细胞内caspase-3活化的荧光光谱分析 总被引:2,自引:0,他引:2
采用CCK-8技术检测发现传统中药消癌平(XAP)对人肺腺癌(ASTC-a-1)细胞的增殖活性具有显著的抑制作用。利用共聚焦扫描荧光显微成像、荧光共振能量转移(FRET)及其受体光漂白技术证实了基于FRET技术构建的SCAT3质粒在ASTC-a-1细胞中的稳定表达。将消癌平加入细胞培养液中培育细胞,并在不同的时间检测活细胞内SCAT3的荧光发射光谱,从而监测细胞中caspase-3的活化状态。实验结果表明:(1)消癌平可以有效抑制人肺腺癌(ASTC-a-1)细胞的增殖活性并诱导细胞的死亡,消癌平对细胞的抑制作用具有剂量依赖性。(2)消癌平处理细胞72 h后,细胞内大量的SCAT3被切割,表明细胞内caspase-3的活化水平明显升高。(3)将含消癌平的细胞培养液与细胞共同培养24 h,然后采用没有消癌平的细胞培养液培养细胞48和72 h后,细胞内SCAT3的光谱没有明显改变,表明消癌平作用细胞24 h内没有显著激活细胞内的caspase-3。 相似文献
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导出了采用L重最大比组合(MRC)和差分相位检测(DPD)方案的高斯最小频移键控(GMSK)卫星移动通信系统在不同信道条件下的误比特率公式,并利用实测数据给出数值计算结果。可用于分析阴影特性对卫星移动信道性能的影响并指导系统设计。 相似文献
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An optimal control method for seismic-excited building structures with multiple time delays is investigated in this paper. The system state equation with multiple time delays is discretized and transformed into a standard discrete form without any explicit time delay by a particular augmenting for state variables. A time-delay controller is then designed based on this standard equation using the discrete optimal control method. Effectiveness of the proposed controller is demonstrated by numerical simulations. Simulation results indicate that a very small time delay may result in the instability of the control system if it is not compensated in the control design. Time delay may be compensated effectively by the proposed controller, in the mean time, an effective control may be obtained. The proposed controller is valid for both small and large time delays. 相似文献
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针对高速移动时分双工长期演进(TDD-LTE,time division duplex-long term evolution)上行单载波频分多址(SC-FDMA,single carrier-frequency division multiple access)系统,提出了一种新型的变换域时变信道估计方法.在所定义的变换域中,基于两块导频符号的信道参数估计,提出利用基于判决导向的非线性内插方法来估计数据符号的信道参数.为了便于SC-FDMA系统均衡,推导给出了变换域信道与频域信道的转换表达式,利用该式可以直接将变换域信道参数估计转化为频域信道响应.计算机仿真结果表明,新方法的性能远远优于传统方法的估计性能,且具有低的计算复杂度. 相似文献
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This paper investigates the impacts of network coding(NC) on user fairness from the network perspective in multiple access channels.Firstly,simultaneous outage is exploited as the metric to evaluate user fairness.To optimize it,a fairness-oriented physical layer NC scheme is proposed,in which users’ signals are superimposed in specific calculated proportion at relay and then decoded by maximal ratio combination(MRC) and serial interference cancellation(SIC) at destination.Furthermore,the influence of power allocation coefficient error on simultaneous outage is also analyzed to reveal its correlation with user fairness.Simulation results indicate that the proposed scheme can supply approximately equal performance promotion for each cooperation-participated user and outperform those without fairness consideration in terms of the employed metric. 相似文献
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