The noninvasive sampling of dermal interstitial fluid (ISF) for the monitoring of clinical biomarkers is a greatly appealing area of research. The identification of molecular biomarkers in biological fluids has been accelerated with -omics analyses but remains limited in ISF because of its time-consuming and complex extraction process. Here, the generation of microneedle (MN) patches made of superabsorbent acrylate-based hydrogels for the rapid sampling of dermal ISF is described to explore its proteome. In depth, iterative optimization allows the identification of novel acrylate-based compositions with the required chemical, mechanical, and biocompatibility properties allowing proteomic analysis of the extracted ISF for the first time after sampling with swelling MNs. The generated MN arrays show no cytotoxic effect, successfully cross the stratum corneum, and can collect up to 6 µL of dermal ISF in 10 min in vivo. Proteomics lead to the detection of 176 clinically relevant biomarkers in the collected samples validating the use of ISF as a relevant bodily fluid for disease monitoring and diagnostic. Importantly, it is discovered that extraction fingerprint is strongly dependent on the MNs chemistry, and thus specific biomarkers could be selectively extracted by tuning the composition of the patch, making the system versatile and specific. 相似文献
A comprehensive numerical tool has been developed for the evaluation of the performances of Radio over Fiber (RoF) links intended for wireless signal distribution.At the transmitter end an appropriate set of rate equations allows to model the optical source as a solitary laser or as an appropriately injection locked laser. The optical channel is modeled putting into account the combined effect of fiber dispersion, laser source non ideal performances (e.g. non-linear effects, frequency chirp), and quadratic detection of the receiving photodiode. The simulation model developed can be a useful tool at the design stage allowing a preliminary evaluation of the characteristics of real RoF links. 相似文献
This paper presents the design and measured performance of a 1.8-GHz power amplifier featuring load mismatch protection and soft-slope power control. Load-mismatch-induced breakdown can be avoided by attenuating the RF power to the final stage during overvoltage conditions. This was accomplished by means of a feedback control system, which detects the peak voltage at the output collector node and clamps its value to a given threshold by varying the circuit gain. The issue of output power control has been addressed as well. To this end, a temperature-compensated bias network is proposed, which allows a moderate power control slope (dB/V) to be achieved by varying the circuit quiescent current according to an exponential law. The nonlinear power amplifier was fabricated using a low-cost silicon bipolar process with a 6.4-V breakdown voltage. It delivers a 33.5-dBm saturated output power with 46% maximum power-added efficiency and 36-dB gain at a nominal 3.5-V supply voltage. The device is able to tolerate a 10:1 load standing-wave ratio up to a 5.1-V supply voltage. Power control slope is lower than 80 dB/V between -15 dBm and the saturated output power level. 相似文献
X-ray photoelectron spectroscopy (XPS) was adopted for the analytical characterization of composite titanium dioxide–poly(vinylidenefluoride) (TiO2–PVDF) films developed for applications in the photocatalytic degradation of pollutants.
The composites were deposited on glass substrates by casting or spin coating from TiO2–PVDF suspensions in dimethylformamide (DMF). XPS data on the TiO2–PVDF surface composition were used to optimize preparation conditions (composition of the TiO2/PVDF suspension, deposition technique) in terms of titanium dioxide surface amount and film stability.
The use of spin-coating deposition and the increase of TiO2 amount in the DMF suspensions were found to improve the titanium surface content, although high TiO2/PVDF ratios led to film instability. PVDF–TiO2 films were also used in preliminary photocatalytic degradation tests on isoproturon, a phenylurea herbicide, under solar UV irradiation; the results were compared to direct photolysis to evaluate the catalytic efficiency of immobilized TiO2 and the role played by the PVDF film during the degradation process. 相似文献
A variable-gain up-conversion mixer for 5-GHz WLAN applications is presented, whose input stage is based on a novel variable
gain transconductor. The proposed topology features reduced power consumption by exploiting dc current reuse for mixer biasing.
Moreover, a new low-consumption control circuit is introduced, which achieves a temperature-stable and linear-in-dB characteristic,
providing a 40-dB dynamic range within ±1 dB gain error. 相似文献
The sulfonated calixarene I8C12 acts as a host for homologous merocyanines Mc1 and Mc2 in organic solvents, exhibiting neither selectivity towards the guest dyes nor solvent dependence of the complexation equilibria. In water, on the contrary, only the lower homologue, Mc1, is solubilized in the presence of the calixarene. A combination of UV–visible and fluorescence spectroscopic and photophysical analysis and MD structural simulation of the calixarene-dye complexes was employed to account for the observations, and suggests that a radical change in the complexation mode occurs upon moving from an organic to an aqueous environment. 相似文献
Cobalt and chromium release in patients undergone a metal-on-metal total hip replacement (THR) is a matter recently discussed and whose we do not have enough information about it yet. In literature there is little data and not completely indicative, in the biological fluids and organs the amounts of released metals are different. This is also due to the fact that cobalt and chromium blood levels can change depending on physical and working activity, individual feeding and metabolism. The results obtained confirm the presence of an increase of cobalt inthe blood of patients after total hip replacement, while the chromium levels are almost alike: average values in patients operated are 4.1 +/- 1.5 microg/L for cobalt (0.3 +/- 0.1 microg/L in the control group) and 4.5 +/- 2.9 microg/L for chromium (4.7 +/- 2.4 microg/L in the control group). In spite of the cobalt values stand below the concentration generally considered dangerous, the difference between the two examined groups points out that a risk exists for the health of these patients. These results must be confirmed by further studies, providing better information and more reliable and biocompatible materials. 相似文献
In this brief work we express the cycle index of the molecular point groups as a function of a limited number of initial geometrical parameters. Such parameters are the number
of elements composing the domain D of sites of substitutions in the molecule belonging to the point group G, and the numbers
of sites of D lying on the symmetry elements
for G. 相似文献
The Na+ transporting properties of the first member of a new class of artificial ionophores, based on a C2-symmetric polyhydroxylated steroid dimer, are described. 相似文献