Synthesis and Expression in Escherichia coli of a Human Neutrophil Activating Protein-1/Interleukin-8 Gene

The complete gene coding for human neutrophilactivating protein-1/interleukin-8 was synthesized using a semi-chemical semi-enzymatic method. The synthetic gene was then overexpressed in Escherichia coli under the temperature-regulated control of the P_RP_L tandem promoters. As determined by SDS-PAGE and densitometry, the overexpressed protein comprised up to 18.5% and 10.9% of the total soluble protein in E. coli cells grown in shake flasks and in batch fermentation, respectively. The recombinant NAP-1/IL-8 was then purified to>95% homogeneity by gel filtration and cation exchange chromatography. The purified protein appeared as a single band on the SDS-PAGE gel and possessed potent chemotactic activity in the concentration of <10 ng/ml, as assayed by the agarose plate method. An early skin reactivity was also observed when the pure NAP-1/IL-8 was injected subcutaneously into the rabbits. The N-terminal 36 amino acid sequence of the recombinant NAP1/IL-8 was determined using the Edman method and was sho     

亚微米光纤制备及非线性增强效应

设计了一种新型条形电加热炉,以普通单模石英光纤为材料,采用熔融拉锥法制备了直径为亚微米量级的拉锥石英光纤.研究了该光纤的受激拉曼散射效应,展望了其在光通信领域的潜在应用.     

高灵敏度多模干涉-异质无芯光纤折射率传感器

基于多模干涉理论和自映像效应,设计了一种高灵敏度多模干涉-异质无芯(SNS)光纤折射率传感器。利用纤芯失配在包层激发的高阶模与无芯光纤中产生的基模耦合产生多模干涉来实现其对折射率的传感测量。应用波束传播法(BPM)数值模拟了传感器在不同折射率条件下光的透射谱,讨论了无芯光纤的长度及外部环境折射率等参数对传感器性能的影响。通过无芯光纤SNS结构传感器的样品制备,测试了多组不同浓度蔗糖溶液下的透射谱,实验结果与数值模拟结果一致。结果表明:在折射率1.330~1.419范围内,透射谷的波长灵敏度达到189nm/RIU,透射率灵敏度达到-40%/RIU。     

人嗜中性白细胞活化蛋白-1/白细胞介素-8基因的半化学半酶促合成及在大肠杆菌的表达

本文采用半化学半酶促法合成了人嗜中性白细胞活化蛋白-1/白细胞介素-8(NAP-1/IL-8)的全基因,并在大肠杆菌系统中利用P_RP_1串联启动子进行了温控型的高效表达,通过SDS聚丙烯酰胺凝胶电泳(SDS-PAGE)检测表明,在摇瓶培养的大肠杆菌中,NAP-1/IL-8的表达量可占菌体可溶性蛋白的18.5％,而在实验室规模的发酵试验中,其表达量则占可溶性蛋白的10.9％.经凝胶过滤层析和阳离子交换层析两个简单的纯化步骤,得到SDS-PAGE纯的重组NAP-1/IL-8,采用琼脂糖平板法进行测定,表明所得NAP-1/IL-8纯品在<10ng/ml的水平上显示出强烈的嗜中性白细胞趋化活性,家兔皮肤试验的结果显示,重组人NAP-1/IL-8可在家兔体内引起早发型超敏反应,采用Edman降解法测定重组人NAP-1/IL-8纯品N末端36个氨基酸的序列,与天然人NAP-1/IL-8的成熟分子完全符合。