腺苷A1和A3受体参与的DRG神经元内Ca2+释放的多光子成像

运用激光共聚焦显微成像技术,通过应用腺苷受体的特异性阻断剂和激动剂,研究腺苷受体对腺苷诱导的大鼠背根神经节(DRG)神经元中Ca2+的动态变化的影响,并且进一步研究了腺苷诱导Ca2+动态变化的剂量相关性。结果显示,腺苷A1和A3受体参与腺苷诱导的DRG神经元Ca2+释放,腺苷能引起DRG神经元内Ca2+浓度升高且呈剂量相关性。研究结果表明,共焦显微成像能有效检测单细胞内离子的动态信号,有助于了解腺苷及其受体参与疼痛过程中细胞离子信号传递的机制。     

Modulating nitric oxide levels in dorsal root ganglion neurons of rat with low-level laser therapy

A novel fiber Bragg grating(FBG)rain gauge is proposed in this paper to achieve high precision rainfall measurement.One core sensitive FBG,a temperature compensation FBG and a mechanical transition system construct this novel FBG rain gauge.Sensing principle of this FBG rain gauge is explained in detail,and its theoretical calculation model is also established,which shows that the relationship between center wavelength of sensitive FBG and external rainfall has very good linearity.To verify its detection performance,the calibration experiment on one prototype of this FBG rain gauge is carried out.After experiment data analysis,the detection precision of this FBG rain gauge is 15.4μm which is almost two orders of magnitude higher than that of the existing rainfall measurement device.The experimental data confirm that this FBG rain gauge can achieve rainfall measurement with high precision.     

低强度激光对软骨细胞增殖的生物刺激作用

八周龄健康SD大鼠的后肢胫骨平台分离培养 软骨细胞,用噻唑蓝(MTT)法检测细胞活性,研究了658、785nm 3种波长 低强度激光对大鼠关节软骨细胞增殖的影响。结果显示,在胎牛血清体积分数为2%和5%的培 养条件下,5J·cm－2的658nm激光 能明显 促进软骨细胞增殖;658、785nm 3种波 长低强度激光均能促进软骨细胞增殖 ,但是具有波长差异性和光剂量相关性。结果表明,低强度激光辐照具有在体治疗软骨损伤 的潜能。