Crystallization of Arthrobacter sp. strain 1C N-(1-D-carboxyethyl)-L-norvaline dehydrogenase and its complex with NAD+

The novel NAD+-linked opine dehydrogenase from a soil isolate Arthrobacter sp. strain 1C belongs to an enzyme superfamily whose members exhibit quite diverse substrate specificites. Crystals of this opine dehydrogenase, obtained in the presence or absence of co-factor and substrates, have been shown to diffract to beyond 1.8 ? resolution. X-ray precession photographs have established that the crystals belong to space group P21212, with cell parameters a = 104.9, b = 80.0, c = 45.5 ? and a single subunit in the asymmetric unit. The elucidation of the three-dimensional structure of this enzyme will provide a structural framework for this novel class of dehydrogenases to enable a comparison to be made with other enzyme families and also as the basis for mutagenesis experiments directed towards the production of natural and synthetic opine-type compounds containing two chiral centres.     

Interferometric studies of a piano soundboard

Electronic speckle pattern interferometry has been used to study the deflection shapes of a piano soundboard. A design for an interferometer that can image such an unstable object is introduced, and interferograms of a piano soundboard obtained using this interferometer are presented. Deflection shapes are analyzed and compared to a finite-element model, and it is shown that the force the strings exert on the soundboard is important in determining the mode shapes and resonant frequencies. Measurements of resonance frequencies and driving-point impedance made using the interferometer are also presented.     

A simple and inexpensive optical technique to help students visualize mode shapes

An imaging technique is introduced that is suitable for visualizing the mode shapes of vibrating structures in an educational setting. The method produces images similar to those obtained using electronic speckle pattern interferometry (ESPI), but it can be implemented for less than 1/10 the cost of a commercial ESPI system, and the apparatus is simple enough that it can be constructed by undergraduate students. This technique allows for real-time visualization of the normal modes and deflection shapes of harmonically vibrating structures, including those with shapes that make generating Chladni patterns with sand or powder impossible. The theory of operation and construction details are discussed.     

Computable error bounds for pointwise derivatives of a Neumann problem

In this paper we discuss the recovery of derivatives and thecomputation of rigorous and useful upper bounds for the pointwiseerror in the recovered derivatives, for finite element approximationsof the Laplace equation with Neumann boundary conditions, especiallyat points close to or on a smooth, curved boundary. We analyzethe dipole image technique for the case of curved boundaries,and show how to compute reliable recovered derivatives and errorbounds even in the limiting case of points lying on the curvedboundary. Numerical experiments show reasonably tight errorbounds for points both close to and away from a curved boundary.     

Development and validation of chromatographic methods for the identification and quantitation of organic compounds leached from a laminated polyolefin material

Chromatographic methods for the identification of organic compounds leached from a plastic material used in solution containers in the pharmaceutical industry are described. Based on a set of compounds identified in extracts of a multilayered polyolefin film, targeted leachables are delineated for accumulation assessments, and methods to perform target quantitation are developed and validated.     

Influence of wall vibrations on the sound of brass wind instruments

The results of an experimental and theoretical investigation of the influence of wall vibrations on the sound of brass wind instruments are presented. Measurements of the transmission function and input impedance of a trumpet, with the bell both heavily damped and freely vibrating, are shown to be consistent with a theory that assumes that the internal pressure causes an oscillation of the diameter of the pipe enclosing the air column. These effects are shown to be most significant in sections where there are flaring walls, which explains why damping these vibrations in cylindrical pipes normally produces no measurable effects.     

Resonance Raman Spectra of [M(6)X(8)Y(6)](2)(-) Cluster Complexes (M = Mo, W; X, Y = Cl, Br, I)

Resonance Raman spectra of the cubic metal-halide complexes having the general formula [M(6)X(8)Y(6)](2)(-) (M = Mo or W; X, Y = Cl, Br, or I) are reported. The three totally symmetric fundamental vibrations of these complexes are identified. The extensive mixing of the symmetry coordinates that compose the symmetric normal modes expected in these systems is not observed. Instead the "group-frequency" approximation is valid. Furthermore, the force constants of both the apical and face-bridging metal-halide bonds are insensitive to the identity of either the metal or the halide. Raman spectra of related complexes with methoxy and benzenethiol groups as ligands are reported along with the structural data for [Mo(6)Cl(8)(SPh)(6)][NBu(4)](2). Crystal data for [Mo(6)Cl(8)(SPh)(6)][NBu(4)](2) at -156 degrees C: monoclinic space group P2(1)/c; a = 12.588(3), b = 17.471(5), c = 20.646(2) ?; beta = 118.53(1) degrees, V = 3223.4 ?(3); d(calcd) = 1.664 g cm(-)(3); Z = 2.