A microfluidic chip‐based fluorescent biosensor for the sensitive and specific detection of label‐free single‐base mismatch via magnetic beads‐based “sandwich” hybridization strategy

A novel microfluidic chip‐based fluorescent DNA biosensor, which utilized the electrophoretic driving mode and magnetic beads‐based “sandwich” hybridization strategy, was developed for the sensitive and ultra‐specific detection of single‐base mismatch DNA in this study. In comparison with previous biosensors, the proposed DNA biosensor has much more robust resistibility to the complex matrix of real saliva and serum samples, shorter analysis time, and much higher discrimination ability for the detection of single‐base mismatch. These features, as well as its easiness of fabrication, operation convenience, stability, better reusability, and low cost, make it a promising alternative to the SNPs genotyping/detection in clinical diagnosis. By using the biosensor, we have successfully determined oral cancer‐related DNA in saliva and serum samples without sample labeling and any preseparation or dilution with a detection limit of 5.6 × 10^?11 M, a RSD (n = 5) < 5% and a discrimination factor of 3.58–4.54 for one‐base mismatch.     

Some basic principles in dynamic theory of viscoelastic materials with voids

According to the basic idea of classical yin-yang complementarity and modern dual-complementarity, in a simple and unified way proposed by Luo, some basic principles in the dynamic theory of viscoelastic materials with voids can be estab- lished systematically. In this paper, an important integral relation in terms of con- volutions is given, which can be considered as the generalized principle of virtual work in mechanics. Based on this relation, it is possible not only to obtain the principle of virtual work and the reciprocal theorem, but also to derive systemati- cally the complementary functionals for the eight-field, six-field, four-field simpli- fied Gurtin-type variational principles and the potential energy-functional for the two-field one in the dynamic theory of viscoelastic materials with voids by the generalized Legendre transformations given in this paper. Furthermore, with this approach, the intrinsic relationship among various principles can be explained clearly.     

Relative position determination of a lunar rover using high-accuracy multi-frequency same-beam VLBI

Multi-frequency same-beam VLBI means that two explorers with a small separation angle are simultaneously observed with the main beam of receiving antennas. In the same-beam VLBI, the differential phase delay between two explorers and two receiving telescopes can be obtained with a small error of several picoseconds. The differential phase delay, as the observable of the same-beam VLBI, gives the separation angular information of the two explorers in the celestial sphere. The two-dimensional relative position on the plane-of-sky can thus be precisely determined with an error of less than 1 m for a distance of 3.8×105 km far away from the earth, by using the differential phase delay obtained with the four Chinese VLBI stations. The relative position of a lunar rover on the lunar surface can be determined with an error of 10 m by using the differential phase delay data and the range data for the lander when the lunar topography near the rover and the lander can be determined with an error of 10 m.     

Hierarchically penalized additive hazards model with diverging number of parameters

In many applications,covariates can be naturally grouped.For example,for gene expression data analysis,genes belonging to the same pathway might be viewed as a group.This paper studies variable selection problem for censored survival data in the additive hazards model when covariates are grouped.A hierarchical regularization method is proposed to simultaneously estimate parameters and select important variables at both the group level and the within-group level.For the situations in which the number of parameters tends to∞as the sample size increases,we establish an oracle property and asymptotic normality property of the proposed estimators.Numerical results indicate that the hierarchically penalized method performs better than some existing methods such as lasso,smoothly clipped absolute deviation(SCAD)and adaptive lasso.     

斜拉索模态试验参数研究

试验模态分析是斜拉桥索力测试中广泛应用的方法。该方法的关键之一是采取合理的试验方案获取可靠的响应信号以识别出正确的索振动频率。本文通过试验,详细讨论了斜拉索模态测试中若干试验参数,如激振类型、激振位置、传感器位置以及频率分辨率对试验结果的影响;比较环境激励与锤击激励的效果,验证了环境激励的方法也可以准确识别索的频率;最后给出了较为合理的斜拉索模态试验方案,用该方案确定的索力与理论计算结果相差不超过 1. 5%。     

Glassy metallic plastics

This paper reports a class of bulk metallic glass including Ce-, LaCe-, CaLi-, Yb-, and Sr-based metallic glasses, which are regarded as glassy metallic plastics because they combine some unique properties of both plastics and metallic alloys. These glassy metallic plastics have very low glass transition temperature (Tg~25oC to 150oC) and low Young’s modulus (~20 GPa to 35 GPa). Similar to glassy plastics, these metallic plastics show excellent plastic-like deformability on macro-, micro- and even nano-scale in their supercooled liquid range and can be processed, such as elongated, compressed, bent, and imprinted at low temperatures, in hot water for instance. Under ambient conditions, they display such metallic properties as high thermal and electric conductivities and excellent mechanical properties and other unique properties. The metallic plastics have potential applications and are also a model system for studying issues in glass physics.     

First-principles study of lithium intercalated bilayer graphene

Lithium intercalated bilayer graphene has been investigated using first-principles density functional theory calculations. Results show that there exist AB and AA stacking sequences for bilayer graphene in which the latter is more favorable for the Li storage and the former will evolve into the latter with the intercalation of Li ions. The relationship between the interlayer distance of two graphene sheets and the intercalated capacity of Li ions is discussed. It is found that structural defect is identified to store Li ions more favorably than pristine bilayer graphene and an isolated C atom vacancy in bilayer graphene can capture three Li ions between two graphene sheets.     

Metallic glassy fibers

A review on the formation and unique physical and mechanical properties of metallic glassy fibers (MGFs) with the diameter ranging from micro to nano scales fabricated by a supercooled liquid extraction method (SLEM) is given. The SLEM method, through driving metallic glass rods in their supercooled liquid region via superplasticity, can fabricate MGFs with precisely designed and controlled size and properties, high structural uniformity and surface smoothness and extreme flexibility. The SLEM method is efficient and the MGFs can be continuously prepared by this method. A parameter f based on the thermal and rheological properties of MG-forming alloys is proposed to control the preparation and size of the fibers. We show that the novel MGFs with superior properties may attract intensive scientific interests and propel more engineering and functional applications.     

A new unequal-weighted triple-frequency first order ionosphere correction algorithm and its application in COMPASS

Science China Physics, Mechanics & Astronomy - As the introduction of triple-frequency signals in GNSS, the multi-frequency ionosphere correction technology has been fast developing. References...     

A toy model for magnetized neutrino-dominated accretion flows

In this paper, we present a simplified model for a magnetized neutrino-dominated accretion flow (NDAF) in which the effect of black hole (BH) spin is taken into account by adopting a set of relativistic correction factors, and the magnetic field is parameterized as β, the ratio of the magnetic pressure to the total pressure. It is found that the disc properties are sensitive to the values of the BH spin and β, and more energy can be extracted from NDAFs by using a faster spin and lower β.