Protein corona composition and precise physiological understanding of differentially expressed proteins are key for identifying disease biomarkers. In this report, we presented a distinctive quantitative proteomics table of molecular cell signaling differentially expressed proteins of corona that formed on iron carbide nanoparticles (NPs). High-performance liquid chromatography/electrospray ionization coupled with ion trap mass analyzer (HPLC/ESI-Orbitrap) and MASCOT helped quantify 142 differentially expressed proteins. Among these proteins, 104 proteins showed upregulated behavior and 38 proteins were downregulated with respect to the control, whereas 48, 32 and 24 proteins were upregulated and 8, 9 and 21 were downregulated CW (control with unmodified NPs), CY (control with modified NPs) and WY (modified and unmodified NPs), respectively. These proteins were further categorized on behalf of their regularity, locality, molecular functionality and molecular masses using gene ontology (GO). A STRING analysis was used to target the specific range of proteins involved in metabolic pathways and molecular processing in different kinds of binding functionalities, such as RNA, DNA, ATP, ADP, GTP, GDP and calcium ion bindings. Thus, this study will help develop efficient protocols for the identification of latent biomarkers in early disease detection using protein fingerprints. 相似文献
The propensity of the new, phenylphosphonate-stabilized polyoxotungstate [(C6H5PVO)2P4W24O92]16− to act as a precursor for new 3d metal-functionalized polyanions has been investigated. Reactions with MnII and CuII induce the formation of the previously unknown polyoxotungstate archetype {P4W27}, isolated as salts of the polyanions [Na⊂{MnII(H2O)}{WO(H2O)}P4W26O98]13− ( 1 ) and [K⊂{CuII(H2O)}{W(OH)(H2O)}P4W27O99]14− ( 2 ), which were characterized in the solid state (single-crystal X-ray diffraction, elemental and TG analyses, IR spectroscopy, SQUID magnetometry) and in aqueous solution (UV/Vis spectroscopy, cyclic voltammetry). 相似文献
Journal of Thermal Analysis and Calorimetry - In this paper, the current, voltage and temperature of the container of an electric current-activated/assisted sintering (ECAS) system are measured and... 相似文献
In this study, we consider parameter‐dependent diffusion eigenvalue problem on time scales. An upper bound on the number of eigenvalues for this problem on a finite time scale is given. 相似文献
Halosulfuron methyl, a fast-acting herbicide and is absorbed into leaf tissue within 1-2 days and translocated through the vascular system, interrupting amino acid production within the plant, can be detected using glassy carbon electrode the technique of adsorptive stripping voltammetry. The adsorptive stripping voltammetric behavior of halosulfuron methyl was investigated in pH range 1.0-10.0. Halosulfuron methyl was irreversibly oxidized at a glassy carbon electrode. Electrochemical techniques including adsorptive stripping voltammetry and cyclic voltammetry were employed to study the oxidation mechanism. The experimental parameters such as the accumulation potential, accumulation time and frequency were optimized. The linear range, detection limit and quantification for halosulfuron methyl were evaluated by adsorptive stripping voltammetry. Under the optimized conditions, the peak current is linear to halosulfuron methyl concentration in the range 4.1-50.0 μg mL−1. Limit of detection and limit of quantification were 1.23 and 4.10 μg mL−1, respectively. The interference of inorganic species and other some pesticides on the voltammetric response have been studied. The applicability to spiked soil and natural water was described and the recoveries for the standards added are 103.8% and 108.2%, respectively. The method is successfully applied for the determination of halosulfuron methyl in commercial formulation. 相似文献
Thickness and composition of thin films can be measured with X- and gamma-rays. In this work, thickness and composition of vanadium pentoxide thin films are investigated by energy dispersive and wavelength dispersive X-ray fluorescence systems. Also, the surface analysis of vanadium pentoxide thin films irradiated with Rhodium Kα X-rays and 59.54?keV gamma-rays emitted from 100?mCi and 5?Ci Americium-241 radioactive sources is performed by scanning electron microscope. It is observed that X- and gamma-rays are destructive for vanadium pentoxide thin films. Also, the composition of vanadium pentoxide thin films changes by irradiation with X- and gamma-rays. 相似文献
Phenolic compounds, such as caffeic acid, trans-ferulic, acid and p-coumaric acid that are commonly found in food products, are beneficial for human health. Cyclodextrins can form inclusion complexes with various organic compounds in which the physiochemical properties of the included organic molecules are changed. In this study, inclusion complexes of three phenolic compounds with β-cyclodextrin were investigated. The complexes were characterized by various analytical methods, including nuclear magnetic resonance (NMR) spectroscopy, Fourier IR (FT-IR) spectroscopy, mass spectrometry, differential scanning calorimetry, and scanning electron microscopy. Results showed that the phenolic compounds used in this study were able to form inclusion complexes in the hydrophobic cavity of β-cyclodextrin by non-covalent bonds. Their physicochemical properties were changed due to the complex formation. In addition, a computational study was performed to find factors that were responsible for binding forces between flavors and β-cyclodextrin. The quantum-mechanical calculations supported the results obtained from experimental studies. Thus, ΔHf for the complex of p-coumaric acid and β-cyclodextrin has been found as ??11.72 kcal/mol, which was about 3 kcal/mol more stable than for inclusion complexes of other flavors. Energies of frontier orbitals (higher occupied molecular orbital (HOMO) and lower unoccupied molecular orbital (LUMO)) were analyzed, and it was found that H-L gap for the complex of p-coumaric acid and β-cyclodextrin had the largest value (8.19 eV) in comparison to other complexes, which confirmed the experimental findings of the most stabile complex.