全文获取类型
收费全文 | 78篇 |
免费 | 2篇 |
专业分类
化学 | 18篇 |
数学 | 38篇 |
物理学 | 24篇 |
出版年
2020年 | 2篇 |
2016年 | 3篇 |
2015年 | 2篇 |
2014年 | 3篇 |
2013年 | 2篇 |
2012年 | 3篇 |
2011年 | 2篇 |
2010年 | 2篇 |
2009年 | 1篇 |
2008年 | 2篇 |
2007年 | 3篇 |
2006年 | 3篇 |
2005年 | 1篇 |
2004年 | 1篇 |
2003年 | 3篇 |
2001年 | 1篇 |
2000年 | 3篇 |
1996年 | 2篇 |
1982年 | 2篇 |
1939年 | 1篇 |
1934年 | 1篇 |
1933年 | 2篇 |
1932年 | 2篇 |
1931年 | 1篇 |
1930年 | 2篇 |
1929年 | 6篇 |
1928年 | 4篇 |
1926年 | 2篇 |
1923年 | 5篇 |
1922年 | 8篇 |
1921年 | 3篇 |
1920年 | 1篇 |
1919年 | 1篇 |
排序方式: 共有80条查询结果,搜索用时 15 毫秒
1.
Spadavecchia J Prete P Lovergine N Tapfer L Rella R 《The journal of physical chemistry. B》2005,109(37):17347-17349
Gold nanoparticles heavily functionalized with oligonucleotides have been used in a variety of DNA detection methods. The optical properties of three-dimensional aggregates of Au nanoparticles in solution or deposited onto suitable surfaces have been analyzed to detect hybridization processes of specific DNA sequences as possible alternatives to fluorescent labeling methods. This paper reports on the preparation of gold nanoparticles directly deposited onto the surface of silicon (Si) and sapphire (Al2O3) substrates by a physical methodology, consisting in the thermal evaporation of a thin Au film and its successive annealing. The method guarantees the preparation of monodispersed single-crystal Au nanoparticles with a strong surface plasmon resonance (SPR) peak centered at about 540 nm. We show that the changes of SPR excitation before and after DNA functionalization and subsequent hybridization of Au nanoparticles immobilized onto Si and Al2O3 substrates can be exploited to fabricate specific biosensors devices in solid phase. 相似文献
2.
3.
Rene F. Roller Ankita Malik Maria A. Carillo Monika Garg Antonella Rella Marie‐Kristin Raulf Bernd Lepenies Peter H. Seeberger Daniel Varn Silva 《Angewandte Chemie (International ed. in English)》2020,59(29):12035-12040
Glypiation is a common posttranslational modification of eukaryotic proteins involving the attachment of a glycosylphosphatidylinositol (GPI) glycolipid. GPIs contain a conserved phosphoglycan that is modified in a cell‐ and tissue‐specific manner. GPI complexity suggests roles in biological processes and effects on the attached protein, but the difficulties to get homogeneous material have hindered studies. We disclose a one‐pot intein‐mediated ligation (OPL) to obtain GPI‐anchored proteins. The strategy enables the glypiation of folded and denatured proteins with a natural linkage to the glycolipid. Using the strategy, glypiated eGFP, Thy1, and the Plasmodium berghei protein MSP119 were prepared. Glypiation did not alter the structure of eGFP and MSP119 proteins in solution, but it induced a strong pro‐inflammatory response in vitro. The strategy provides access to glypiated proteins to elucidate the activity of this modification and for use as vaccine candidates against parasitic infections. 相似文献
4.
5.
van Der Voort M Rella CW van Der Meer LF Akimov AV Dijkhuis JI 《Physical review letters》2000,84(6):1236-1239
We present results of the first vibrational photon-echo, transient-grating, and temperature dependent transient-bleaching experiments on a-Si:H. Using these techniques, and the infrared light of a free electron laser, the vibrational population decay and phase relaxation of the Si-H stretching mode were investigated. Careful analysis of the data indicates that the vibrational energy relaxes directly into Si-H bending modes and Si phonons, with a distribution of rates determined by the amorphous host. Conversely, the pure dephasing appears to be single exponential, and can be modeled by dephasing via two-phonon interactions. 相似文献
6.
We report the observation of giant quantum coherence effects in the localized modes of ionized hydrogen in synthetic fluorite. Infrared free induction decay experiments on the substitutional H- center show dramatic modulations at negative delay times due to interference between multiple vibrational levels. Spectrally resolving the degenerate four wave mixing signal allows unambiguous assignments of the participating vibrational states. The dependence of the signal intensity upon the delay path between the exciting free electron laser pulses can be accounted for in terms of the resonant third order polarization with a common dephasing time for the excited states. 相似文献
7.
A. P. Caricato A. Cretí A. Luches M. Lomascolo M. Martino R. Rella D. Valerini 《Laser Physics》2011,21(3):588-597
Various kinds of zinc oxide (ZnO) nanostructures, such as columns, pencils, hexagonal pyramids, hexagonal hierarchical structures,
as well as smooth and rough films, were grown by pulsed laser deposition using KrF and ArF excimer lasers, without use of
any catalyst. ZnO films were deposited at substrate temperatures from 500 to 700°C and oxygen background pressures of 1, 5,
50, and 100 Pa. Quite different morphologies of the deposited films were observed using scanning electron microscopy when
different laser wavelengths (248 or 193 nm) were used to ablate the bulk ZnO target. Photoluminescence studies were performed
at different temperatures (down to 7 K). The gas sensing properties of the different nanostructures were tested against low
concentrations of NO2. The variation in the photoluminescence emission of the films when exposed to NO2 was used as transduction mechanism to reveal the presence of the gas. The nanostructured films with higher surface-to-volume
ratio and higher total surface available for gas adsorption presented higher responses, detecting NO2 concentrations down to 3 ppm at room temperature. 相似文献
8.
Daniela Fico Antonio Pennetta Giulia Rella Antonella Savino Valentina Terlizzi Giuseppe Egidio De Benedetto 《Journal of Raman spectroscopy : JRS》2016,47(3):321-328
A combined analytical approach has been applied to the wall paintings, dated from 10th to 14th centuries, of the Santi Stefani crypt at Vaste (Lecce, Southern Italy). These paintings are a precious testimony of Medieval art in Southern Italy. However, the church shows problems of damp as well as clear evidences of flora, fungi and mold presence, and there is little knowledge of the pictorial methodologies used. Raman spectroscopy allowed to determine the palette and to reconstruct the worksite and the chronological sequence of the various paint layers. Kaolinite, calcite, carbon black, hematite, massicot, goethite, indigo and azurite were identified as pigments along with synthetic pigments, like phthalocyanine blue and chrome yellow. Attenuated total reflectance‐Fourier transform infrared spectroscopy suggested the presence of egg as a binder in some pictorial layers. The conservation state of the crypt is poor, and detachments of pigmented layers are frequent because of the presence of subflorescence and efflorescence: nitrate, sulfate and chloride salts have been identified spectroscopically and quantified by ion chromatography. The extensive use of kaolinite in Santi Stefani, actually not uncommon in Medieval art, is observed for the first time in a crypt of Puglia: its use to stabilize some pigments and to improve their adhesion on substrate is proposed. Copyright © 2015 John Wiley & Sons, Ltd. 相似文献
9.
10.
Marco Moschetta Michele Telegrafo Leonarda Rella Amato Antonio Stabile Ianora Giuseppe Angelelli 《Magnetic resonance imaging》2014