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Usui K Hiratsuka A Shiseki K Maruo Y Matsushima T Takahashi K Unuma Y Sakairi K Namatame I Ogawa Y Yokoyama K 《Electrophoresis》2006,27(18):3635-3642
We developed a polymeric 2-DE chip system. The chip consisted of an IEF region, an SDS-PAGE region, a valveless connection port, and a sample introduction port. A "junction structure" as a valveless connection port, which allowed separating and connecting the first- and second-dimensional gels, was fabricated between their regions. A "solution inlet" as a sample introduction port was fabricated to perform the liquid and sample introductions without solution leakage. Simultaneous sample monitoring was performed using the on-chip detection system. The performances of the system were demonstrated using commercially available proteins as a standard specimen and tissue-extracted proteins as the real samples. All procedures were employed without any movement of relocation part. This new 2-D separation system realized improved labor-intensive operations and a reduced experimental time. 相似文献
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Hidekatsu Yokoyama Toshiyuki Sato Tateaki Ogata Hiroaki Ohya-Nishiguchi Hitoshi Kamada 《Journal of magnetic resonance (San Diego, Calif. : 1997)》1997,129(2):201-206
A signal detector of longitudinally detected ESR (LODESR) is independent of the resonant frequency. We developed anin vivoLODESR spectrometer operating in the regions of 300, 700, and 900 MHz. Using this apparatus, we estimated signal intensities at different operating frequencies obtained from non- or high-dielectric loss phantoms that contained nitroxide radical solutions and from live rats that had received a nitroxide radical. Our result, higher signal intensities in the high-dielectric loss samples (such as physiological saline solution and animals) at a lower frequency, shows that the influence of a decrease in dielectric loss dominates over the signal reduction caused by smaller Zeeman splitting. We believe that this finding strongly supports anin vivoESR resonant frequency that tends to be low. 相似文献
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Tateaki Wakamiya Keiko Yamanoi Masahiro Nishikawa Tetsuo Shiba 《Tetrahedron letters》1985,26(39):4759-4760
Bulgecinine, a new proline type amino acid in bulgecins, was synthesized stereospecifically by use of D-glucose as a chiral precursor. 相似文献
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A new cyclopropyl amino acid, carnosadine, was isolated from red alga Grateloupia carnosa and the structure was determined to be 1-amino-2-guanidino-methylcylopropane-1-carboxylic acid from the results of NMR, mass spectrometry and coloring tests. 相似文献
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[reaction: see text] Novel rod-shaped polycyano-oligo(phenyleneethynylene)s were synthesized by Pd cross-coupling reaction. Polycyano groups were found to greatly improve the emission efficiency (Phi(f)) of OPEs. By the end donor modification, we achieved the creation of very intense blue light-emitting fluorophore with the SMe group (Phi(f) = 0.972, log epsilon 4.89, lambda(em) 455 nm) and very intense yellow light-emitting fluorophore with the NMe(2) group (Phi(f) = 0.999, log epsilon 4.75, lambda(em) 555 nm). Contrasting Phi(f) solvent dependency of 6 and 7 and a linear relationship between Phi(f) and sigma(p)-X over the whole region of sigma(p)-X were also found. 相似文献
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Shigeya KobayashiYoshihiro Yamaguchi Tateaki WakamiyaYoshio Matsubara Kunihisa SugimotoZen-ichi Yoshida 《Tetrahedron letters》2003,44(7):1469-1472
Simple members of arene-azaarenecyclynes as a novel family of geometrically-controlled and shape-persistent azamacrocycles have been synthesized. Noteworthy is the specific recognition function for Sb(V). The synthesized azamacrocycles, in particular the Sb(V) complex, have unusually strong light-emitting property. 相似文献
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Mitamura K Sakai T Nakai R Wakamiya T Iida T Hofmann AF Ikegawa S 《Analytical and bioanalytical chemistry》2011,400(7):2061-2072
Previous work from this laboratory has reported the chemical synthesis of N-acetylcysteine (NAC) conjugates of natural bile acids (BAs) and shown that such novel conjugates can be formed in vivo in
rats to which NAC has been administered. The subsequent fate of such novel conjugates is not known. One possible biotransformation
is sulfation, a major pathway for BAs N-acylamidates in patients with cholestatic liver disease. Here, we report the chemical synthesis of the 3-sulfates of the
S-acyl NAC conjugates of five natural BAs (cholic, chenodeoxycholic, deoxycholic, ursodeoxycholic, and lithocholic). We also
measured the sulfation of N-acetylcysteine–natural bile acid (BA-NAC) conjugates when they were incubated with a rat liver cytosolic fraction. The chemical
structures of the BA-NAC 3-sulfates were confirmed by proton nuclear magnetic resonance, as well as by means of electrospray
ionization-linear ion trap mass spectrometry with negative-ion detection. Upon collision-induced dissociation of singly and
doubly charged deprotonated molecules, structurally informative product ions were observed. Using a triple-stage quadrupole
instrument, selected reaction monitoring analyses by monitoring characteristic transition ions allowed the achievement of
a highly sensitive and specific assay. When BA-NACs were incubated with a rat liver cytosolic fraction to which 3’-phosphoadenosine
5’-phosphosulfate was added, sulfation occurred, but the dominant reaction was hydrolysis of the S-acyl linkage to form the unconjugated BAs. Subsequent sulfation occurred at C-3 on the unconjugated BAs that had been formed
from the BA-NACs. Such sulfation was proportional to the hydrophobicity of the unconjugated bile acid. Thus, NAC conjugates
of BAs as well as their C-3 sulfates if formed in vivo are rapidly hydrolyzed by cytosolic enzymes. 相似文献