排序方式: 共有32条查询结果,搜索用时 234 毫秒
1.
Alan Rigter Jan PM Langeveld Drophatie Timmers-Parohi Jorg G Jacobs Peter LJM Moonen Alex Bossers 《BMC biochemistry》2007,8(1):6
Background
The common event in transmissible spongiform encephalopathies (TSEs) or prion diseases is the conversion of host-encoded protease sensitive cellular prion protein (PrPC) into strain dependent isoforms of scrapie associated protease resistant isoform (PrPSc) of prion protein (PrP). These processes are determined by similarities as well as strain dependent variations in the PrP structure. Selective self-interaction between PrP molecules is the most probable basis for initiation of these processes, potentially influenced by chaperone molecules, however the mechanisms behind these processes are far from understood. We previously determined that polymorphisms do not affect initial PrPC to PrPSc binding but rather modulate a subsequent step in the conversion process. Determining possible sites of self-interaction could elucidate which amino acid(s) or amino acid sequences contribute to binding and further conversion into other isoforms. To this end, ovine – and bovine PrP peptide-arrays consisting of 15-mer overlapping peptides were probed with recombinant sheep PrPC fused to maltose binding protein (MBP-PrP). 相似文献2.
3.
4.
P. M. L. O. Scholte M. Tegze F. Van Der Woude K. H. J. Buschow I. Vincze 《Hyperfine Interactions》1986,27(1-4):437-440
The quadrupole splitting in alloys is determined by the structural and electronic properties of the alloy. Only in cases where
the density of states at the Fermi level is constant or known as a function of the concentration, relevant structural information
can be obtained from the quadrupole splitting. 相似文献
5.
Metal/superconductor/semiconductor (Ag/Hg-1212/CdSe) hetero-nanostructures have been fabricated using pulse-electrodeposition
technique and are characterized by X-ray diffraction (XRD), full-width at half-maximum (FWHM) and scanning electron microscopy
(SEM) studies. The junction capacitance of Ag/Hg-1212, Hg-1212/CdSe and Ag/Hg-1212/CdSe heterojunctions is measured in dark
and under laser irradiation at room temperature. The nature of the junction formed and built-in-junction potentials were determined.
The increase in carrier concentration across the junction due to photo-irradiation has been observed. 相似文献
6.
One of the innovative technological directions for the high-temperature superconductors has been persued by fabricating the
heteroepitaxial multilayer structures such as superconductor-semiconductor heterostructures. In the present investigation,
metal/superconductor/semiconductor (Ag/Tl-2223/CdSe) hetero-nanostructures have successfully been fabricated using dc electrodeposition
technique and were characterized by X-ray diffraction (XRD), full-width at half-maximum (FWHM) and scanning electron microscopy
(SEM) studies. The measurement of junction capacitance as a function of biasing voltage was used for the estimation of junction
built-in-potential (V
D) and to study the charge distribution in a heterojunction. The Mott-Schottky plots were measured for each junction in dark
and under the photo-irradiation. The effect of laser irradiation on C-V characteristics of hetero-nanostructure has been studied. 相似文献
7.
Th. G. Scholte 《Journal of Polymer Science.Polymer Physics》1971,9(9):1553-1577
As is well known, the chemical potentials of polymer and solvent in solution and, hence, the Flory-Huggins interaction parameter χ can be determined from scattered light intensities from dilute and concentrated solutions of the polymer in the solvent concerned. Preferably, measurements should be performed at temperatures as low as possible, provided the temperature exceeds the cloudpoint for the concentration used. It is shown that the lower the temperature and, consequently the higher the scattered light intensity, the better is the accuracy of the parameters obtained. At each temperature the scattered light intensity shows a maximum at some concentration. Below the theta temperature the ratio of scattered light intensity and concentrations also shows a maximum at some concentration. The values and the concentrations of these maxima for various temperatures enable the maximum of the spinodal to be determined. The spinodal itself can be determined by an extrapolation procedure of the reciprocal scattered light intensities. Measurements have been performed with three narrow-distribution polystyrene samples in cyclohexane. On the basis of the results, χ, and its dependence on concentration, temperature, and molecular weight can be determined to high accuracy. 相似文献
8.
Th. G. Scholte 《Journal of Polymer Science.Polymer Physics》1972,10(3):519-526
Starting from the Lorentz-Lorenz equation, Heller, a few years ago, advanced a method for calculating the partial specific volume of a substance in a dilute solution from the refractive index increment of the solution and the specific refraction of the solute. It is shown that this method, based on graphical extrapolation, can be considerably simplified. Further, a method is indicated by which the partial specific volume of a substance over the whole range of concentrations can be calculated in an analogous manner. The method is checked and elucidated with reference to some experimental examples. 相似文献
9.
Despite the burgeoning interest in the various biological functions and consequent therapeutic potential of the vast number of oligosaccharides found in nature on glycoproteins and cell surfaces, the development of combinatorial carbohydrate chemistry has not progressed as rapidly as expected. The reason for this imbalance is rooted in the difficulty of oligosaccharide assembly and analysis that renders synthesis a rather cumbersome endeavor. Parallel approaches that generate series of analogous compounds rather than real libraries have therefore typically been used. Since generally low affinity is obtained for interactions between carbohydrate receptors and modified oligosaccharides designed as mimetics of natural carbohydrate ligands, glycopeptides have been explored as alternative mimics. Glycopeptides have been proven in many cases to be superior ligands with higher affinity for a receptor than the natural carbohydrate ligand. High-affinity glycopeptide ligands have been found for several types of receptors including the E-, P-, and L-selectins, toxins, glycohydrolases, bacterial adhesins, and the mannose-6-phosphate receptor. Furthermore, the assembly of glycopeptides is considerably more facile than that of oligosaccharides and the process can be adapted to combinatorial synthesis with either glycosylated amino acid building blocks or by direct glycosylation of peptide templates. The application of the split and combine approach using ladder synthesis has allowed the generation of very large numbers of compounds which could be analyzed and screened for binding of receptors on solid phase. This powerful technique can be used generally for the identification and analysis of the complex interaction between the carbohydrates and their receptors. 相似文献
10.
Dr. Alexander Scholte Christoph Hübner Dr. Dieter Ströhl Olaf Scheufler Dr. Steffen Czich Julia M. Börke Dr. Gerhard Hildebrand Prof. Dr. Klaus Liefeith 《ChemistryOpen》2021,10(9):889-895
Due to their special chemical structure, tetraether lipids (TEL) represent essential elements of archaeal membranes, providing these organisms with extraordinary properties. Here we describe the characterization of a newly isolated structural element of the main lipids. The TEL fragment GDNT-β-Glu was isolated from Sulfolobus metallicus and characterized in terms of its chemical structure by NMR- and MS-investigations. The obtained data are dissimilar to analogically derived established structures – in essence, the binding relationships in the polar head group are re-determined and verified. With this work, we provide an important contribution to the structure elucidation of intact TEL also contained in other Sulfolobus strains such as Solfulobus acidocaldarius and Sulfolobus solfataricus. 相似文献