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1.
When normal or SV40-transformed Balb/c 3T3 cells are treated with the Ca++-specific chelator EGTA, they round up and pull away from their footpad adhesion sites to the serum-coated tissue culture substrate, as shown by scanning electron microscope studies. Elastic membranous retraction fibers break upon culture agitation, leaving adhesion sites as substrate-attached material (SAM) (Cells leave "footprints" of substrate adhesion sites during movement by a very similar process.) SAM contains 1-2% of the cell's total protein and phospholipid content and 5-10% of its glucosamine-radiolabeled polysaccharide, most of which is glycosaminoglycan (GAG). By one- and two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis, there is considerable enrichment in SAM for specific GAGs; for the glycoprotein fibronectin; and for the cytoskeletal proteins actin, myosin, and the subunit protein of the 10 nm-diameter filaments. Fibrillar fibronectin of cellular origin and substratum-bound fibronectin of serum origin (cold-insoluble globulin, CIg) have been visualized by immunofluorescence microscopy. The GAG composition in SAM has been examined under different cellular growth and attachment conditions. Heparan sulfate content correlates with glycopeptide content (derived from glycoprotein). Newly attaching cells deposit SAM with principally heparan sulfate and fibronectin and little of the other GAGs. Hyaluronate and chrondroitin proteoglycans are coordinately deposited in SAM as cells begin spreading and movement over the substrate. Cells attaching to serum-coated or CIg-coated substrates deposited SAM with identical compositions. The proteoglycan nature of the GAGs in SAM has been examined, as well as the ability of proteoglycans to form two classes of reversibly dissociable "supramolecular complexes" - one class with heparan sulfate and glycopeptide-containing material and the second with hyaluronate-chondroitin complexes. Enzymatic digestion of "intact" SAM with trypsin or testicular hyaluronidase indicates that (1) only a small portion of long-term radiolabeled fibronectin and cyto-skeletal protein is bound to the substrate via hyaluronate or chondroitin classes of GAG; (2) most of the fibronectin, cytoskeletal protein and heparan sulfate coordinately resist solubilization; and (3) newly synthesized fibronectin, which is metabolically labile in SAM, is linked to SAM by hyaluronate- and/or chondroitin-dependent binding. All of our studies indicate that heparan sulfate is a direct mediator of adhesion of cells to the substrate, possibly by binding to both cell-surface fibronectin and substrate-bound CIg in the serum coating; hyaluronate-chondroitin complexes in SAM appear to be most important in motility of cells by binding and labilizing fibronectin at the periphery of footpad adhesions, with subsequent cytoskeletal disorganization. 相似文献
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A high-power, low-noise photodetector, in conjunction with a current shunt actuator, is used in an ac-coupled servo to stabilize the intensity of a 10-W cw Nd:YAG laser. A relative intensity noise of 1 x 10(-8) Hz(-1/2) at 10 Hz is achieved. 相似文献
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We describe a novel technique for contrast enhancement in optical coherence tomography (OCT) that makes possible molecular-specific imaging for what is believed to be the first time. A pump-probe technique is employed in which a pulsed pump laser is tuned to ground-state absorption in a molecule of interest. The location of the target molecule population is derived from the resulting transient absorption of OCT sample-arm light acting as probe light. A signal processing technique for three-dimensional localization of the transient absorption signal is described, and preliminary results exhibiting OCT contrast from methylene blue dye in multilayer and scattering phantoms are presented. 相似文献
7.
Imaging and velocimetry of the human retinal circulation with color Doppler optical coherence tomography 总被引:3,自引:0,他引:3
Noninvasive monitoring of blood flow in retinal microcirculation may elucidate the progression and treatment of ocular disorders, including diabetic retinopathy, age-related macular degeneration, and glaucoma. Color Doppler optical coherence tomography (CDOCT) is a technique that allows simultaneous micrometer-scale resolution cross-sectional imaging of tissue microstructure and blood flow in living tissues. CDOCT is demonstrated for the first time in living human subjects for bidirectional blood-flow mapping of retinal vasculature. 相似文献
8.
We formulate a Volterra equation model to describe the effect of harvesting on populations with delayed recruitment and age-dependent mortality. This model is more complicated mathematically than the unharvested model, and we describe some numerical simulations which indicate the possible qualitative behaviours. 相似文献
9.
The reactions of cis-(NH3)2PtL2 (L = 1-MeT or 1-MeU, the anions of 1-methylthymine or 1-methyluracil respectively) in water with various salts of Fe(II), Fe(III), Co(II), Ni(II), Cu(II) or Ag(I) have produced 14 new heteronuclear complexes of the general types: cis-[(NH3)2PtL2ML2Pt(NH3)2]Xn·mH2O or cis-(NH3)2PtL2MXn·mH2O, and also with silver(I) perchlorate a complex of stoichiometry cis-{(NH3)2Pt(1-MeU)2}2· 3AgClO4·6H2O. Comparisons are made with previously reported heteronuclear species derived from cis-(NH3)2PtL2 and factors influencing the type of heteronuclear complex formed are discussed, particularly the versatility of binding patterns with Ag(I). A tetranuclear structure is suggested for the compound of stoichiometry cis-(NH3)2Pt(1-MeU)2·FeSO4·3H2O involving iron(II) ions linked by sulphate bridges. The electronic spectra of the trinuclear complexes cis-[(NH3)2Pt(1-MeU)2M(1-MeU)2Pt(NH3)2] (NO3)2·mH2O (M = Fe, Co or Ni) are reported. They show that the trans-MO4Pt2 geometry results in a very severely trans-elongated ligand field about the central metal ion, M. This conclusion is supported by the Mössbauer spectrum in the case of the iron(II) complex. The X- and Q-band EPR spectra of the Fe(III) analogue are also reported. 相似文献
10.
For part 29, see Rogerset al. (1989). 相似文献