排序方式: 共有13条查询结果,搜索用时 0 毫秒
1.
Landa Purushottam Unnikrishnan V. B. Dattatraya Gautam Rawale Mansi Gujrati Surya Dev Mishra Sajeev T. K. Neelesh C. Reddy Srinivasa Rao Adusumalli Ram Kumar Mishra Vishal Rai 《Chemical science》2020,11(48):13137
Analytically pure proteins are indispensable for diverse applications, including therapeutics. Here, we report a methodology where a single amino acid, glycine, enables metal-free protein purification. This robust platform is enabled by a Gly-tag resin for site-specific capture, enrichment, and release through chemically triggered C–C bond dissociation by resonance-assisted electron density polarization.Gly-tag resin precisely captures and releases a protein with one glycine at the N-terminus. The user-friendly protocol delivers analytically pure protein free of metal contaminants. 相似文献
2.
Ramesh-Babu Adusumalli Ulrich Müller Hedda Weber Thomas Roeder Herbert Sixta Wolfgang Gindl 《Macromolecular Symposia》2006,244(1):83-88
Summary: A tensile testing set-up was developed for the determination of the elastic modulus, tensile strength, and failure strain of single regenerated cellulose fibres. Since the accuracy of strain measurement is crucial for the measured elastic modulus and failure strain, strain measurements were performed mechanically and with a non-contacting optical method in parallel. The optical validation of mechanical strain measurement showed an agreement of measured strain >99%, confirming the accuracy and usefulness of the set-up and sample geometry developed for the test series. 相似文献
3.
Mr. Kingshuk Mukhuti Dr. V. N. K. B. Adusumalli Mr. Heramba V. S. R. M. Koppisetti Prof. Bhavtosh Bansal Prof. Venkataramanan Mahalingam 《Chemphyschem》2020,21(15):1731-1736
Precise assessment of temperature is crucial in many physical, technological, and biological applications where optical thermometry has attracted considerable attention primarily due to fast response, contactless measurement route, and electromagnetic passivity. Rare-earth-doped thermographic phosphors that rely on ratiometric sensing are very efficient near and above room temperature. However, being dependent on the thermally-assisted migration of carriers to higher excited states, they are largely limited by the quenching of the activation mechanism at low temperatures. In this paper, we demonstrate a strategy to pass through this bottleneck by designing a linear colorimetric thermometer by which we could estimate down to 4 K. The change in perceptual color fidelity metric provides an accurate measure for the sensitivity of the thermometer that attains a maximum value of 0.86 K−1. Thermally coupled states in Er3+ are also used as a ratiometric sensor from room temperature to ∼140 K. The results obtained in this work clearly show that Yb3+−Er3+ co-doped NaGdF4 microcrystals are a promising system that enables reliable bimodal thermometry in a very wide temperature range from ultralow (4 K) to ambient (290 K) conditions. 相似文献
4.
Ramesh-Babu Adusumalli Rejin Raghavan Rudy Ghisleni Tanja Zimmermann Johann Michler 《Applied Physics A: Materials Science & Processing》2010,100(2):447-452
The deformation and failure of the secondary cell wall of Spruce wood was studied by in-situ SEM compression of micropillars
machined by the focused ion beam technique. The cell wall exhibited yield strength values of approximately 160 MPa and large
scale plasticity. High resolution SEM imaging post compression revealed bulging of the pillars followed by shear failure.
With additional aid of cross-sectional analysis of the micropillars post compression, a model for deformation and failure
mechanism of the cell wall has been proposed. The cell wall consists of oriented cellulose microfibrils with high aspect ratio
embedded in a hemicellulose-lignin matrix. The deformation of the secondary wall occurs by asymmetric out of plane bulging
because of buckling of the microfibrils. Failure of the cell wall following the deformation occurs by the formation of a shear
or kink band. 相似文献
5.
An improved high-performance liquid chromatographic method with UV detection at 313 nm has been developed for quantitation of ranitidine in 100 microliter of rat plasma over the range 25 to 1000 ng/ml. To each sample were added the internal standard (metiamide) and 2 M NaOH. After dichloromethane extraction, the nitrogen-dried extracts were reconstituted in the mobile phase of 0.01 M phosphate buffer-triethylamine-methanol-water (530:5:390:75 v/v). Chromatography on mu Bondapak C18 with quantitation by peak height ratios showed an analyte recovery of 97%; a limit of detection of 10 ng/ml; a precision of 1-10% and an accuracy of 1-5%. About 90 samples can be processed in 24 h. 相似文献
6.
Comparison of molecular orientation and mechanical properties of lyocell fibre tow and staple fibres
Ramesh-Babu Adusumalli Jozef Keckes Klaus J. Martinschitz Peter Boesecke Hedda Weber Thomas Roeder Herbert Sixta Wolfgang Gindl 《Cellulose (London, England)》2009,16(5):765-772
Samples of lyocell fibres were taken in the form of filaments from fibre tow of potentially infinite length and in their final
condition of staple fibres. Mechanical testing showed comparable tensile strength, but a 50% lower modulus of elasticity for
staple fibres and a higher elongation at break compared to filaments from fibre tow. Structural investigation by means of
synchrotron wide angle X-ray scattering and birefringence measurement revealed a significantly lower degree of preferred orientation
together with less fibre straightness for staple fibres than for filaments. It is concluded that plastic deformation during
the processing of staple fibres from filaments induces permanent changes in the orientation of cellulose chains in the fibres,
which in turn is responsible for the observed differences in mechanical performance. 相似文献
7.
Dattatraya Gautam Rawale Kalyani Thakur Pranav Sreekumar Sajeev T. K. Ramesh A. Srinivasa Rao Adusumalli Ram Kumar Mishra Vishal Rai 《Chemical science》2021,12(19):6732
The conservation of chemoselectivity becomes invalid for multiple electrophilic warheads during protein bioconjugation. Consequently, it leads to unpredictable heterogeneous labeling of proteins. Here, we report that a linchpin can create a unique chemical space to enable site-selectivity for histidine and aspartic acid modifications overcoming the pre-requisite of chemoselectivity.Linchpin-enabled promiscuous electrophile uncovers an unchartered reactivity landscape for the precision engineering of proteins. 相似文献
8.
Srinivasa Rao Adusumalli Dattatraya Gautam Rawale Kalyani Thakur Landa Purushottam Neelesh C. Reddy Neetu Kalra Sanjeev Shukla Vishal Rai 《Angewandte Chemie (International ed. in English)》2020,59(26):10332-10336
The necessity for precision labeling of proteins emerged during the efforts to understand and regulate their structure and function. It demands selective attachment of tags such as affinity probes, fluorophores, and potent cytotoxins. Here, we report a method that enables single‐site labeling of a high‐frequency Lys residue in the native proteins. At first, the enabling reagent forms stabilized imines with multiple solvent‐accessible Lys residues chemoselectively. These linchpins create the opportunity to regulate the position of a second Lys‐selective electrophile connected by a spacer. Consequently, it enables the irreversible single‐site labeling of a Lys residue independent of its place in the reactivity order. The user‐friendly protocol involves a series of steps to deconvolute and address chemoselectivity, site‐selectivity, and modularity. Also, it delivers ordered immobilization and analytically pure probe‐tagged proteins. Besides, the methodology provides access to antibody‐drug conjugate (ADC), which exhibits highly selective anti‐proliferative activity towards HER‐2 expressing SKBR‐3 breast cancer cells. 相似文献
9.
Strong Single‐Band Blue Emission from Colloidal Ce3+/Tm3+‐Doped NaYF4 Nanocrystals for Light‐Emitting Applications 下载免费PDF全文
Venkata N. K. B. Adusumalli Shyam Sarkar Dr. Venkataramanan Mahalingam 《Chemphyschem》2015,16(11):2312-2316
An intense single‐band blue emission at λ=450 nm is observed from Tm3+ ions through Ce3+ sensitization, for the first time, in colloidal Ce3+/Tm3+‐doped NaYF4 nanocrystals. The intense Tm3+ emission through broad‐band excitation is advantageous for developing luminescent nanocomposites because they can be easily incorporated into polymers. The composites can easily be coated over UV light‐emitting diodes (LEDs) to develop phosphor‐based blue LEDs. 相似文献
10.
Jakob Schwiedrzik Rejin Raghavan Markus Rüggeberg Silla Hansen Juri Wehrs Ramesh B. Adusumalli 《哲学杂志》2016,96(32-34):3461-3478
Based on a combination of micropillar compression experiments and modelling of the secondary cell wall (cw) using continuum micromechanics, the shear yield stress of the polymer matrix is identified for both normal and compression wood of Norway spruce. It is shown that the model is able to capture the differences in mechanical properties between the two tissues based on the knowledge of composition of the samples, microfibril angle, as well as phase properties on the nanometer scale. By testing an isolated piece of the cell wall with a homogeneous and uniaxial stress field on the micrometer scale and using the micromechanical model to determine average stress fields on the nanometer scale, it is possible to identify the shear yield stress of the polymer matrix in wood, which was found to be in the range of 14.9–17.5 MPa for normal and compression wood. It was shown that this corresponds to a stress in the lignin phase of approx. 17 MPa. This combined study thus demonstrates a new approach for validating multiscale models predicting yield properties with uniaxial experiments at the microscale and measuring phase properties of inhomogeneous materials by a combination of modelling and experimental approaches. 相似文献